CD25 Rat mAb - Primary antibody, for In vivo regulatory T cell depletion,Flow cytometry various applications, from Mouse, generated in Rat, specific to CD25, IgG1
CD25 Rat mAb - Primary antibody, for In vivo regulatory T cell depletion,Flow cytometry various applications, from Mouse, generated in Rat, specific to CD25, IgG1
CD25 Rat mAb - Primary antibody, for In vivo regulatory T cell depletion,Flow cytometry various applications, from Mouse, generated in Rat, specific to CD25, IgG1
Synonyms
Anti-mouse CD25 (IL-2Rα)-InVivo; In Vivo MAb anti-mouse CD25 (IL-2Rα); CD25 Rat mAb; CD25, Il2r, Ly-43; Interleukin 2 receptor alpha chain antibody; CD25 antibody; CD25 antigen antibody; IDDM10 antibody; IL 2 receptor alpha subunit antibody; IL-2 recept
Specifications & Purity
ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host Species
Rat
Host species
Rat
Specificity
CD25
Application
In vivo regulatory T cell depletion,Flow cytometry
Immunogen
IL-2-dependent cytolytic mouse T cell clone B6.1
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Application: In vivo regulatory T cell depletion; Flow cytometry
Recommended Isotype Control: In Vivo MAb rat IgG1 isotype control, anti-horseradish peroxidase
The PC-61.5.3 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The PC-61.5.3 antibody has been shown to inhibit the binding of IL-2 to both the low and high affinity IL-2 receptor forms. Additionally, the PC-61.5.3 antibody is commonly used to deplete CD4+FoxP3+ T regulatory cells in vivo.
Product Properties
Antibody Type
Primary antibody
Clonality
Monoclonal antibodies
Isotype
IgG1
Light Chain Type
lambda
SDS-PAGE
150kDa
Purification Method
Protein A High Salt
Source
Hybridoma supernatant
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
ZJ23F0500128