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CD27 Rat mAb - Primary antibody, specific to CD27, Rat IgG2a

    Application:
  • Animal Model
  • Flow Cytometry
  • Functional Assay
  • IP
  • Stimulation
Features and benefitsView More
  • Host species: Rat
  • Species reactivity(Reacts with): Mouse
  • Isotype: Rat IgG2a
  • Clone Number: RM27-3E5
  • Conjugation: Unconjugated
Item Number
Ab094343
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab094343-10μg
10μg
In stock
$29.90
Ab094343-50μg
50μg
In stock
$79.90
Ab094343-100μg
100μg
In stock
$109.90
Ab094343-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$799.90

mAb (RM27-3E5); Rat anti Mouse CD27 Antibody; Flow, IP, in vivo CD27 stimulation, in vitro CD27 stimulation; Unconjugated

Basic Description

Product NameCD27 Rat mAb - Primary antibody, specific to CD27, Rat IgG2a
SynonymsS152 | Tnfrsf7 | CD27 antigen | CD27 molecule | CD27 | MGC20393 | S152CD27L receptor | T cell activation antigen CD27 | T cell activation antigen S152 | T14 | TNFRSF7 | TNFRSF7T-cell activation antigen CD27 | Tp55 | Tumor necrosis factor receptor superfam
Specifications & PurityCarrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host speciesRat
SpecificityCD27
ImmunogenMouse CD27-human IgG1 Fc fusion protein
ConjugationUnconjugated
GradeAzide Free, Carrier Free, Validated
Product Description

Rat anti Mouse CD27 Antibody, Monoclonal (RM27-3E5), could be used for Flow, IP, in vivo CD27 stimulation, in vitro CD27 stimulation and so on.
Application
Flow: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
in vivo CD27 stimulation: Use at an assay dependent concentration.
in vitro CD27 stimulation: Use at an assay dependent concentration.
Protein Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Product Properties

IsotypeRat IgG2a
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
ConcentrationSee COA
Storage TempStore at 2-8°C,Freeze prohibited
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.

Images

CD27 Rat mAb (Ab094343) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD27 with CD27 Rat mAb (Ab094343) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

CD27 Rat mAb (Ab094343) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD27 (red) with CD27 Rat mAb (Ab094343) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

CD27 Tbio CD27 antigen (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences
CD27 antigen agonistAGONISTALA3713333CD27 antigenSINGLE PROTEINHomo sapiens

PubMed: [1]


Application

ApplicationDilution info
IPUse at an assay dependent concentration.

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Use at an assay dependent concentration.

Flow CytometryUse at an assay dependent concentration.

">

Use at an assay dependent concentration.

in vivo CD27 stimulation

Use at an assay dependent concentration.

in vitro CD27 stimulation

Use at an assay dependent concentration.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Enter lot number to search for analytical chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607318Certificate of AnalysisJun 25, 2024 Ab094343
ZJ24F0607317Certificate of AnalysisJun 25, 2024 Ab094343
ZJ24F0607316Certificate of AnalysisJun 25, 2024 Ab094343

Related Documents

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