pAb; Rabbit anti Human CD276 Antibody; WB, IHC, Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab094381-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab094381-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $100.90 | |
| Ab094381-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $199.90 | |
| Ab094381-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $349.90 | |
| Ab094381-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
pAb; Rabbit anti Human CD276 Antibody; WB, IHC, Flow; Unconjugated
| Product Name | CD276 Antibody - Primary antibody, specific to CD276, Rabbit IgG |
|---|---|
| Synonyms | 4Ig B7 H3 antibody; 4Ig-B7-H3 antibody; AU016588 antibody; B7 H3 antibody; B7 homolog 3 antibody; B7-H3 antibody; B7H3 antibody; B7RP-2 antibody; CD_antigen=CD276 antibody; CD276 antibody; CD276 antigen antibody; CD276 molecule antibody; CD276_HUMAN antib |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | CD276 |
| Immunogen | Recombinant CD276 expressed in E.coli (AA 47-219) |
| Positive Control | WB: HeLa, LNCAP, HEK293 and MCF7 cell lysates. IHC: Human Liver cancer tissue, Human Kidney tissue. Flow: CHO-K1 transfected with Human CD276. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Rabbit anti Human CD276 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified and Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
CD276 Antibody (Ab094381) - Western Blot
All lanes: CD276 Antibody (Ab094381) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 92 kDa
Exposure time: 71.7 s
CD276 Antibody (Ab094381) - Flow Cytometry
CHO-K1 transfected with human CD276 (Stable transfection) was stained with CD276 Antibody (Ab094381) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by Cy5-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.01-3 µg/mL |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901087 | Certificate of Analysis | Sep 18, 2023 | Ab094381 |
| 1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10): (2265-70). [PMID:12975309] |
| 2. Loo D, Alderson RF, Chen FZ, Huang L, Zhang W, Gorlatov S, Burke S, Ciccarone V, Li H, Yang Y et al.. (2012) Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity.. Clin Cancer Res, 18 (14): (3834-45). [PMID:22615450] |
| 3. Chapoval AI, Ni J, Lau JS, Wilcox RA, Flies DB, Liu D, Dong H, Sica GL, Zhu G, Tamada K et al.. (2001) B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production.. Nat Immunol, 2 (3): (269-74). [PMID:11224528] |
| 4. Castriconi R, Dondero A, Augugliaro R, Cantoni C, Carnemolla B, Sementa AR, Negri F, Conte R, Corrias MV, Moretta L et al.. (2004) Identification of 4Ig-B7-H3 as a neuroblastoma-associated molecule that exerts a protective role from an NK cell-mediated lysis.. Proc Natl Acad Sci USA, 101 (34): (12640-5). [PMID:15314238] |
| 5. Ling, Vincent V and 6 more authors.. (2003) Duplication of primate and rodent B7-H3 immunoglobulin V- and C-like domains: divergent history of functional redundancy and exon loss.. Genomics, [PMID:12906861] |
| 6. Steinberger, Peter P and 9 more authors.. (2004) Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:14764704] |
| 7. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 8. Wang, Liqing L and 7 more authors.. (2005) B7-H3 promotes acute and chronic allograft rejection.. European journal of immunology, [PMID:15682454] |
| 9. Kim, Jean J and 8 more authors.. (2005) Constitutive and inducible expression of b7 family of ligands by human airway epithelial cells.. American journal of respiratory cell and molecular biology, [PMID:15961727] |
| 10. Petroff, Margaret G MG, Kharatyan, Elza E, Torry, Donald S DS and Holets, Lesya L.. (2005) The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta.. The American journal of pathology, [PMID:16049332] |
| 11. Zhang, Guangbo G, Dong, Qiuming Q, Xu, Ying Y, Yu, Gehua G and Zhang, Xueguang X.. (2005) B7-H3: another molecule marker for Mo-DCs?. Cellular & molecular immunology, [PMID:16274630] |
| 12. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743] |
| 13. Hashiguchi, Masaaki M and 5 more authors.. (2008) Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2) is a counter-receptor for B7-H3 and enhances T cell responses.. Proceedings of the National Academy of Sciences of the United States of America, (29): [PMID:18650384] |
| 14. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 15. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
