| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab006726-10μg | 10μg | In stock | $19.90 | |
| Ab006726-50μg | 50μg | In stock | $39.90 | |
| Ab006726-100μg | 100μg | In stock | $59.90 | |
| Ab006726-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $519.90 |
mAb (CD28.2); Mouse anti Human CD28 Antibody; Flow; Unconjugated
| Product Name | CD28 Mouse mAb - Primary antibody, specific to CD28, Mouse IgG1 |
|---|---|
| Synonyms | CD28 antigen (Tp44) | CD28 antigen | CD28 molecule | CD28 | MGC138290 | T-cell-specific surface glycoprotein CD28 | Tp44 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD28 |
| Immunogen | Human CD28 transfected cell line |
| Positive Control | Flow: Human peripheral blood lymphocytes. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD28 Antibody, Monoclonal (CD28.2), could be used for Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
CD28 Mouse mAb (Ab006726) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD28 (red) with CD28 Mouse mAb (Ab006726) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD28 Mouse mAb (Ab006726) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD28 with CD28 Mouse mAb (Ab006726) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD28 Mouse mAb (Ab006726) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD28 (red) with CD28 Mouse mAb (Ab006726) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1μg/100w cells "> 1μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505153 | Certificate of Analysis | May 07, 2024 | Ab006726 |
| ZJ24F0505152 | Certificate of Analysis | May 07, 2024 | Ab006726 |
| ZJ24F0505151 | Certificate of Analysis | May 07, 2024 | Ab006726 |
| 1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039] [10.1021/op500134e] |
| 2. Suchard SJ, Davis PM, Kansal S, Stetsko DK, Brosius R, Tamura J, Schneeweis L, Bryson J, Salcedo T, Wang H et al.. (2013) A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety.. J Immunol, 191 (9): (4599-610). [PMID:24081989] [10.1021/op500134e] |
| 3. Tomoko Hayashi, Ji-Hun Mo, Xing Gong, Cyprian Rossetto, Angela Jang, Lucinda Beck, Gregory I Elliott, Irina Kufareva, Ruben Abagyan, David H Broide, Jongdae Lee, Eyal Raz,. (2007-11-16) 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis.. Proceedings of the National Academy of Sciences of the United States of America, 104 ((47)): ( 18619-18624 ). [PMID:18003900] |
| 4. Lee, K P KP and 5 more authors.. (1990) The genomic organization of the CD28 gene. Implications for the regulation of CD28 mRNA expression and heterogeneity.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2162892] |
| 5. Aruffo, A A and Seed, B B.. (1987) Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2825196] |
| 6. Blotta, M H MH, Marshall, J D JD, DeKruyff, R H RH and Umetsu, D T DT.. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8617933] |
| 7. Ling, V V and 9 more authors.. (2001) Assembly and annotation of human chromosome 2q33 sequence containing the CD28, CTLA4, and ICOS gene cluster: analysis by computational, comparative, and microarray approaches.. Genomics, [PMID:11735222] |
| 8. Deshpande, Manisha M, Venuprasad, K K, Parab, Pradeep B PB, Saha, Bhaskar B and Mitra, Debashis D.. (2002) A novel CD28 mRNA variant and simultaneous presence of various CD28 mRNA isoforms in human T lymphocytes.. Human immunology, [PMID:11916166] |
| 9. Mikolajczak, Sebastian A SA and 5 more authors.. (2004) The modulation of CD40 ligand signaling by transmembrane CD28 splice variant in human T cells.. The Journal of experimental medicine, (5): [PMID:15067037] |
| 10. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186] |
| 11. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 12. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
| 13. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
