| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab189661-10T (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10T | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $53.90 | |
| Ab189661-25T | 25T | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $106.90 | |
| Ab189661-100T | 100T | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $259.90 |
mAb (UCHT1); Mouse anti Human CD3 Antibody (AF488); Flow
| Product Name | CD3 Mouse mAb (AF488) |
|---|---|
| Synonyms | CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a |
| Specifications & Purity | ExactAb™, Validated, Ex:490nm, Em:525nm, 5 μL/test |
| Biochemical and Physiological Mechanisms | The CD3 complex mediates signal transduction. |
| Host species | Mouse |
| Specificity | CD3 |
| Immunogen | Human CD3ε |
| Positive Control | Flow: Human peripheral blood lymphocytes |
| Conjugation | Alexa Fluor® 488 |
| Grade | Em:525nm, Ex:490nm, ExactAb™, Validated |
| Product Description | Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein G purified |
| Concentration | 5 μL/test |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245).Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry |
5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. "> 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
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