CD3 Mouse mAb (PE) - Primary antibody, specific to CD3E, Mouse IgG2a

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2a
  • Clone Number: HIT3a
  • Conjugation: PE
Item Number
Ab179705
Grouped product items
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Ab179705-10T (Trial Size)
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10T
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Ab179705-25T
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Ab179705-100T
100T
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$59.90

mAb (HIT3a); Mouse anti Human CD3 Antibody (PE); Flow

Basic Description

Product NameCD3 Mouse mAb (PE) - Primary antibody, specific to CD3E, Mouse IgG2a
SynonymsCD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps
Specifications & PurityExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 5μL/test
Host speciesMouse
SpecificityCD3E
ImmunogenHuman thymocytes
Positive ControlFlow: Jurkat
ConjugationPE
GradeAzide Free, Em:575nm, Ex:565nm, ExactAb™, Validated
Product Description

Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Product Properties

IsotypeMouse IgG2a
Light Chain Typekappa
Purification MethodProtein A purified
FormLiquid
Concentration5μL/test
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD3 Mouse mAb (PE) (Ab179705) - Flow Cytometry
Flow cytometry analysis of CD3 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (PE) (Ab179705) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry 5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. ">

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303584Certificate of AnalysisMar 22, 2024 Ab179705
ZJ24F0303583Certificate of AnalysisMar 22, 2024 Ab179705
ZJ24F0303582Certificate of AnalysisMar 22, 2024 Ab179705

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