ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species
Mouse
Specificity
CD34
Immunogen
Permanent human cell line from a patient with myeloid leukaemia.
Positive Control
Flow: Human peripheral blood cells.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Mouse anti Human CD34 Antibody, Monoclonal (4H11), could be used for Flow and so on. Application: Flow: 0.25 μg/100w cells Protein Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.
Images
CD34 Mouse mAb (Ab094638) - Flow Cytometry Flow Cytometry analysis of KG-1a cells labelling CD34 (red) with CD34 Mouse mAb (Ab094638) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
1.Simmons, D L DL, Satterthwaite, A B AB, Tenen, D G DG and Seed, B B.. (1992) Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells.. Journal of immunology (Baltimore, Md. : 1950), (1):[PMID:1370171]
2.Satterthwaite, A B AB, Burn, T C TC, Le Beau, M M MM and Tenen, D G DG.. (1992) Structure of the gene encoding CD34, a human hematopoietic stem cell antigen.. Genomics, [PMID:1374051]
3.Fackler, M J MJ, Civin, C I CI, Sutherland, D R DR, Baker, M A MA and May, W S WS.. (1990) Activated protein kinase C directly phosphorylates the CD34 antigen on hematopoietic cells.. The Journal of biological chemistry, (5):[PMID:1694174]
4.Sutherland, D R DR and 6 more authors.. (1988) Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.. Leukemia, [PMID:2462139]
5.Nakamura, Y Y, Komano, H H and Nakauchi, H H.. (1993) Two alternative forms of cDNA encoding CD34.. Experimental hematology, [PMID:7678811]
6.Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18):[PMID:16710414]
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