mAb (E11); Mouse anti Human CD35 Antibody; Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab006580-10μg | 10μg | In stock | $26.90 | |
| Ab006580-50μg | 50μg | In stock | $69.90 | |
| Ab006580-100μg | 100μg | In stock | $109.90 | |
| Ab006580-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (E11); Mouse anti Human CD35 Antibody; Flow; Unconjugated
| Product Name | CD35 Mouse mAb - Primary antibody, specific to CD35, Mouse IgG1 |
|---|---|
| Synonyms | C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN; C3 binding protein antibody; C3b/C4b receptor antibody; C3BR antibody; C4BR antibody; CD 35 antibody; CD35 antibody; CD35 antigen antibody; complement component (3b/4b) rece |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD35 |
| Immunogen | Human CD35 from Acute monocytic leukemia cells and normal blood monocytes. |
| Positive Control | Flow: Human peripheral blood lymphocytes. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human CD35 Antibody, Monoclonal (E11), could be used for Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
CD35 Mouse mAb (Ab006580) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD35 (red) with CD35 Mouse mAb (Ab006580) at 5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD35 Mouse mAb (Ab006580) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD35 with CD35 Mouse mAb (Ab006580) conjugated with biotin (right panel) at 0.02 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD35 Mouse mAb (Ab006580) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD35 (red) with CD35 Mouse mAb (Ab006580) conjugated with biotin at 0.02 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 0.25μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505393 | Certificate of Analysis | May 11, 2024 | Ab006580 |
| ZJ24F0505392 | Certificate of Analysis | May 11, 2024 | Ab006580 |
| ZJ24F0505391 | Certificate of Analysis | May 11, 2024 | Ab006580 |
| 1. Yaskanin, D D DD, Thompson, L F LF and Waxman, F J FJ.. (1992) Distribution and quantitative expression of the complement receptor type 1 (CR1) on human peripheral blood T lymphocytes.. Cellular immunology, [PMID:1534036] |
| 2. Wong, W W WW and 8 more authors.. (1989) Structure of the human CR1 gene. Molecular basis of the structural and quantitative polymorphisms and identification of a new CR1-like allele.. The Journal of experimental medicine, (1): [PMID:2564414] |
| 3. Wong, W W WW, Klickstein, L B LB, Smith, J A JA, Weis, J H JH and Fearon, D T DT.. (1985) Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2933745] |
| 4. Klickstein, L B LB and 5 more authors.. (1987) Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristics of C3/C4 binding proteins.. The Journal of experimental medicine, (1): [PMID:2951479] |
| 5. Hourcade, D D, Miesner, D R DR, Atkinson, J P JP and Holers, V M VM.. (1988) Identification of an alternative polyadenylation site in the human C3b/C4b receptor (complement receptor type 1) transcriptional unit and prediction of a secreted form of complement receptor type 1.. The Journal of experimental medicine, (1): [PMID:2971757] |
| 6. Klickstein, L B LB and 5 more authors.. (1988) Identification of distinct C3b and C4b recognition sites in the human C3b/C4b receptor (CR1, CD35) by deletion mutagenesis.. The Journal of experimental medicine, (1): [PMID:2972794] |
| 7. Takemura, S S and 7 more authors.. (1984) C3b receptor (CR1) on erythrocytes in various diseases.. Immunology letters, [PMID:6233222] |
| 8. Vik, D P DP and Wong, W W WW.. (1993) Structure of the gene for the F allele of complement receptor type 1 and sequence of the coding region unique to the S allele.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8245463] |
| 9. Moulds, J M JM, Reveille, J D JD and Arnett, F C FC.. (1996) Structural polymorphisms of complement receptor 1 (CR1) in systemic lupus erythematosus (SLE) patients and normal controls of three ethnic groups.. Clinical and experimental immunology, [PMID:8706338] |
| 10. Moulds, J M JM and 14 more authors.. (2001) Molecular identification of Knops blood group polymorphisms found in long homologous region D of complement receptor 1.. Blood, (1): [PMID:11313284] |
| 11. Moulds, J M JM and 7 more authors.. (2002) Expansion of the Knops blood group system and subdivision of Sl(a).. Transfusion, [PMID:11896343] |
| 12. Cockburn, Ian A IA and 8 more authors.. (2004) A human complement receptor 1 polymorphism that reduces Plasmodium falciparum rosetting confers protection against severe malaria.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:14694201] |
| 13. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 14. Ogembo, Javier G JG and 6 more authors.. (2013) Human complement receptor type 1/CD35 is an Epstein-Barr Virus receptor.. Cell reports, (21): [PMID:23416052] |
| 15. Török, Katalin K, Dezső, Balázs B, Bencsik, András A, Uzonyi, Barbara B and Erdei, Anna A.. (2015) Complement receptor type 1 (CR1/CD35) expressed on activated human CD4+ T cells contributes to generation of regulatory T cells.. Immunology letters, [PMID:25742728] |
