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CD4 Mouse mAb (Biotin) - Primary antibody, specific to CD4, Mouse IgG2b

    Application:
  • Flow Cytometry
Features and benefitsView More
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2b
  • Clone Number: OKT4
  • Conjugation: Biotin
Item Number
Ab180130
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab180130-10μg (Trial Size)
Apply for free trial size(?)
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10μg
In stock
$29.90
Ab180130-25μg
25μg
In stock
$49.90
Ab180130-100μg
100μg
In stock
$79.90

mAb (OKT4); Mouse anti Human CD4 Antibody (Biotin); Flow

Basic Description

Product NameCD4 Mouse mAb (Biotin) - Primary antibody, specific to CD4, Mouse IgG2b
SynonymsCD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti
Specifications & PurityValidated, 0.5 mg/mL
Host speciesMouse
SpecificityCD4
ImmunogenHuman peripheral T cells.
Positive ControlFlow: Human peripheral blood lymphocytes
ConjugationBiotin
GradeValidated
Product Description

Application:
Flow: ≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

IsotypeMouse IgG2b
Purification MethodProtein G purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD4 Mouse mAb (Biotin) (Ab180130) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (Biotin) (Ab180130) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

CD4 Mouse mAb (Biotin) (Ab180130) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (Biotin) (Ab180130) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences
T-cell surface antigen CD4 otherOTHERALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens
T-cell surface antigen CD4 inhibitorINHIBITORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

DOI: [1]


ISBN: [1]


PubMed: [1] , [2]


T-cell surface antigen CD4 inhibitorINHIBITORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

PubMed: [1] , [2]


T-cell surface antigen CD4 agonistAGONISTALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiensBinds epitope in the IgG-like C2 type 1 domain distinct from binding region for other ligands

PMC: [1]


PubMed: [1]


T-cell surface antigen CD4 inhibitorINHIBITORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

PubMed: [1]


T-cell surface antigen CD4 negative modulatorNEGATIVE MODULATORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

Patent: [1]


PubMed: [1]


T-cell surface antigen CD4 negative modulatorNEGATIVE MODULATORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

PubMed: [1]


T-cell surface antigen CD4 inhibitorINHIBITORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

PubMed: [1]


T-cell surface antigen CD4 binding agentBINDING AGENTALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

PubMed: [1] , [2]


T-cell surface antigen CD4 inhibitorINHIBITORALA2754T-cell surface antigen CD4SINGLE PROTEINHomo sapiens

PubMed: [1]


Application

ApplicationDilution info
Flow Cytometry≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

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≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates

C of A & Other Certificates

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506306Certificate of AnalysisMay 30, 2024 Ab180130
ZJ24F0506305Certificate of AnalysisMay 30, 2024 Ab180130
ZJ24F0506304Certificate of AnalysisMay 30, 2024 Ab180130
ZJ24R0500566Certificate of AnalysisMay 17, 2024 Ab180130

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