| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab170295-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $16.90 |
mAb (RPA-T4); Mouse anti Human CD4 Antibody; Flow; Unconjugated
| Product Name | CD4 Mouse mAb - Primary antibody, for Flow,ELISA,SPR,Invivo/Invitro various applications, from Human, generated in Human, specific to CD4, Mouse IgG1 |
|---|---|
| Synonyms | CD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host Species | Human |
| Host species | Mouse |
| Specificity | CD4 |
| Application | Flow,ELISA,SPR,Invivo/Invitro |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD4 Antibody, Monoclonal (RPA-T4), could be used for Flow and so on. Application Flow: < 0.5 μg/100w cells Protein Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Mouse IgG1 |
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot. |
CD4 Mouse mAb (Ab170295) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD4 with CD4 Mouse mAb (Ab170295) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD4 Mouse mAb (Ab170295) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD4 (red) with CD4 Mouse mAb (Ab170295) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
CD4 Mouse mAb (Ab170295) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD4 (red) with CD4 Mouse mAb (Ab170295) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | < 0.5µg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102385 | Certificate of Analysis | Jan 10, 2024 | Ab170295 |
| ZJ24F0102384 | Certificate of Analysis | Jan 10, 2024 | Ab170295 |
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| 2. Wilkinson RA, Pincus SH, Song K, Shepard JB, Weaver AJ, Labib ME, Teintze M.. (2013) Improved guanide compounds which bind the CXCR4 co-receptor and inhibit HIV-1 infection.. Bioorg Med Chem Lett, 23 (7): (2197-2201). [PMID:23434419] [10.1016/j.bmcl.2013.01.107] |
| 3. Lumangtad LA, Bell TW.. (2020) The signal peptide as a new target for drug design.. Bioorg Med Chem Lett, 30 (10): (127115-127115). [PMID:32209293] [10.1016/j.bmcl.2020.127115] |
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| 10. Crise, B B, Buonocore, L L and Rose, J K JK.. (1990) CD4 is retained in the endoplasmic reticulum by the human immunodeficiency virus type 1 glycoprotein precursor.. Journal of virology, [PMID:2214026] |
| 11. Ryu, S E SE and 9 more authors.. (1990) Crystal structure of an HIV-binding recombinant fragment of human CD4.. Nature, (29): [PMID:2247146] |
| 12. Carr, S A SA and 7 more authors.. (1989) Protein and carbohydrate structural analysis of a recombinant soluble CD4 receptor by mass spectrometry.. The Journal of biological chemistry, (15): [PMID:2592374] |
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| 14. Maddon, P J PJ and 5 more authors.. (1985) The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family.. Cell, [PMID:2990730] |
| 15. Littman, D R DR, Maddon, P J PJ and Axel, R R.. (1988) Corrected CD4 sequence.. Cell, (18): [PMID:3263213] |
| 16. Lusso, P P and 5 more authors.. (1994) CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:7909607] |
| 17. Winkel, K K, Sotzik, F F, Vremec, D D, Cameron, P U PU and Shortman, K K.. (1994) CD4 and CD8 expression by human and mouse thymic dendritic cells.. Immunology letters, [PMID:8088877] |
| 18. Aiken, C C, Konner, J J, Landau, N R NR, Lenburg, M E ME and Trono, D D.. (1994) Nef induces CD4 endocytosis: requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain.. Cell, (11): [PMID:8124721] |
| 19. Ansari-Lari, M A MA and 7 more authors.. (1996) A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.. Genome research, [PMID:8723724] |
| 20. Ansari-Lari, M A MA, Shen, Y Y, Muzny, D M DM, Lee, W W and Gibbs, R A RA.. (1997) Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.. Genome research, [PMID:9074930] |
| 21. Wu, H H, Kwong, P D PD and Hendrickson, W A WA.. (1997) Dimeric association and segmental variability in the structure of human CD4.. Nature, (29): [PMID:9168119] |
| 22. Kwong, P D PD and 5 more authors.. (1998) Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody.. Nature, (18): [PMID:9641677] |
| 23. Zeitlmann, L L, Sirim, P P, Kremmer, E E and Kolanus, W W.. (2001) Cloning of ACP33 as a novel intracellular ligand of CD4.. The Journal of biological chemistry, (23): [PMID:11113139] |
| 24. Fragoso, Roben R and 5 more authors.. (2003) Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:12517957] |
| 25. Sharma, Deepak D and 7 more authors.. (2005) Protein minimization of the gp120 binding region of human CD4.. Biochemistry, (13): [PMID:16331979] |
| 26. Löwenberg, Mark M and 7 more authors.. (2006) Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN.. EMBO reports, [PMID:16888650] |
| 27. Bernstein, Helene B HB and 5 more authors.. (2006) CD4 expression on activated NK cells: ligation of CD4 induces cytokine expression and cell migration.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:16951326] |
| 28. Lindwasser, O Wolf OW, Chaudhuri, Rittik R and Bonifacino, Juan S JS.. (2007) Mechanisms of CD4 downregulation by the Nef and Vpu proteins of primate immunodeficiency viruses.. Current molecular medicine, [PMID:17346169] |
| 29. Garron, Marie-Line ML and 5 more authors.. (2008) Structural basis for the interaction between focal adhesion kinase and CD4.. Journal of molecular biology, (1): [PMID:18078954] |
| 30. Wang, Xin Xiang XX and 7 more authors.. (2011) Affinity maturation of human CD4 by yeast surface display and crystal structure of a CD4-HLA-DR1 complex.. Proceedings of the National Academy of Sciences of the United States of America, (20): [PMID:21900604] |
| 31. Fernandes, Rosa Anita and 11 more authors.. (2019) Complete Multilineage CD4 Expression Defect Associated With Warts Due to an Inherited Homozygous CD4 Gene Mutation.. Frontiers in immunology, [PMID:31781092] |
