mAb (RPA-T4); Mouse anti Human CD4 Antibody; Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab170295-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $16.90 |
mAb (RPA-T4); Mouse anti Human CD4 Antibody; Flow; Unconjugated
| Product Name | CD4 Mouse mAb - Primary antibody, for Flow,ELISA,SPR,Invivo/Invitro various applications, from Human, generated in Human, specific to CD4, Mouse IgG1 |
|---|---|
| Synonyms | CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; CD 4 antibody; CD4 (L3T4) antibody; CD4 antibody; CD4 antigen (p55) antibody; CD4 antigen antibody; |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host Species | Human |
| Host species | Mouse |
| Specificity | CD4 |
| Application | Flow,ELISA,SPR,Invivo/Invitro |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD4 Antibody, Monoclonal (RPA-T4), could be used for Flow and so on. Application Flow: < 0.5 μg/100w cells Protein Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Mouse IgG1 |
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot. |
CD4 Mouse mAb (Ab170295) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD4 with CD4 Mouse mAb (Ab170295) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD4 Mouse mAb (Ab170295) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD4 (red) with CD4 Mouse mAb (Ab170295) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
CD4 Mouse mAb (Ab170295) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD4 (red) with CD4 Mouse mAb (Ab170295) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | < 0.5µg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102385 | Certificate of Analysis | Jan 10, 2024 | Ab170295 |
| ZJ24F0102384 | Certificate of Analysis | Jan 10, 2024 | Ab170295 |
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| 17. Winkel, K K, Sotzik, F F, Vremec, D D, Cameron, P U PU and Shortman, K K.. (1994) CD4 and CD8 expression by human and mouse thymic dendritic cells.. Immunology letters, [PMID:8088877] |
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| 19. Ansari-Lari, M A MA and 7 more authors.. (1996) A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.. Genome research, [PMID:8723724] |
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| 21. Wu, H H, Kwong, P D PD and Hendrickson, W A WA.. (1997) Dimeric association and segmental variability in the structure of human CD4.. Nature, (29): [PMID:9168119] |
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| 24. Fragoso, Roben R and 5 more authors.. (2003) Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:12517957] |
| 25. Sharma, Deepak D and 7 more authors.. (2005) Protein minimization of the gp120 binding region of human CD4.. Biochemistry, (13): [PMID:16331979] |
| 26. Löwenberg, Mark M and 7 more authors.. (2006) Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN.. EMBO reports, [PMID:16888650] |
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| 29. Garron, Marie-Line ML and 5 more authors.. (2008) Structural basis for the interaction between focal adhesion kinase and CD4.. Journal of molecular biology, (1): [PMID:18078954] |
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| 31. Fernandes, Rosa Anita and 11 more authors.. (2019) Complete Multilineage CD4 Expression Defect Associated With Warts Due to an Inherited Homozygous CD4 Gene Mutation.. Frontiers in immunology, [PMID:31781092] |
