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CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1

Features and benefits
  • Short Overview:

    mAb (HI30); Mouse anti Human CD45 Antibody; Flow; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
    Application:
  • Flow Cytometry
Item Number
Ab094902
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab094902-10μg
10μg
In stock
$16.90
Ab094902-50μg
50μg
In stock
$39.90
Ab094902-100μg
100μg
In stock
$69.90
Ab094902-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$619.90
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mAb (HI30); Mouse anti Human CD45 Antibody; Flow; Unconjugated

View related series
Click to view target information: PTPRC
Accession#:P08575 CD45 Gene ID:5788 PTPRC

Basic Description

Product NameCD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1
SynonymsB220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotei
Specifications & PurityExactAb™, Validated, Carrier Free, Lot by Lot
Host speciesMouse
SpecificityCD45
ImmunogenPeripheral blood mononuclear cells
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD45 Antibody, Monoclonal (HI30), could be used for Flow and so on.
Application:
Flow: < 0.5 μg/100w cells
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Raji cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Jurkat cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094902) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094902) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PTPRC Tchem Leukocyte common antigen (2317 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
Flow Cytometry

< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0102526Certificate of AnalysisJan 18, 2024 Ab094902
ZJ24F0102525Certificate of AnalysisJan 18, 2024 Ab094902
ZJ24F0102524Certificate of AnalysisJan 18, 2024 Ab094902

Related Documents

 SDS

 Specification Sheet

FAQs and Articles

FAQ
Flow cytometry cell cycle analysis using propidium iodide DNA staining
Categories: Protocols
Tags:
Propidium Iodide Flow Cytometry Antibody Cell Cycle
Experimental Guide of Sample Preparation for Flow Cytometry
Categories: Protocols
Tags:
Protein sample preparation Flow Cytometry Antibody FACS

References

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