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CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1

Carrier Free

Validated

ExactAb™

Lot by Lot

Application:

Flow Cytometry

Features and benefits

Host species: Mouse
Species reactivity(Reacts with): Human
Isotype: Mouse IgG1
Clone Number: HI30
Conjugation: Unconjugated

Item Number

Ab094902

Grouped product items
SKU	Size	Availability	Price
Ab094902-10μg	10μg	In stock	$16.90
Ab094902-50μg	50μg	In stock	$39.90
Ab094902-100μg	100μg	In stock	$69.90
Ab094902-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$619.90

mAb (HI30); Mouse anti Human CD45 Antibody; Flow; Unconjugated

View related series

Click to view target information: PTPRC

Accession#:P08575 CD45 Gene ID:5788 PTPRC

Basic Description

Product Name	CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1
Synonyms	B220 \| CD_antigen: CD45 \| CD45 antigen \| CD45 \| CD45R \| EC 3.1.3.48 \| EC:3.1.3.48 \| GP180 \| LCA \| L-CA \| Leukocyte common antigen \| LY5 \| protein tyrosine phosphatase, receptor type, c polypeptide \| PTPRC \| receptor-type tyrosine-protein phosphatase C \| T
Specifications & Purity	ExactAb™, Validated, Carrier Free, Lot by Lot
Host species	Mouse
Specificity	CD45
Immunogen	Peripheral blood mononuclear cells
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD45 Antibody, Monoclonal (HI30), could be used for Flow and so on. Application: Flow: < 0.5 μg/100w cells Protein Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Raji cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Jurkat cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094902) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094902) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
Flow Cytometry	< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102526	Certificate of Analysis	Jan 18, 2024	Ab094902
ZJ24F0102525	Certificate of Analysis	Jan 18, 2024	Ab094902
ZJ24F0102524	Certificate of Analysis	Jan 18, 2024	Ab094902

FAQs and Articles

FAQ

Flow cytometry cell cycle analysis using propidium iodide DNA staining

Categories: Protocols

Tags:

Propidium Iodide Flow Cytometry Antibody Cell Cycle

Experimental Guide of Sample Preparation for Flow Cytometry

Categories: Protocols

Tags:

Protein sample preparation Flow Cytometry Antibody FACS

References

1. Chen YT, Seto CT.. (2002) Divalent and trivalent alpha-ketocarboxylic acids as inhibitors of protein tyrosine phosphatases.. J Med Chem, 45 (18): (3946-3952). [PMID:12190316] [10.1021/jm020093q]
2. Andersen HS, Olsen OH, Iversen LF, Sørensen AL, Mortensen SB, Christensen MS, Branner S, Hansen TK, Lau JF, Jeppesen L, Moran EJ, Su J, Bakir F, Judge L, Shahbaz M, Collins T, Vo T, Newman MJ, Ripka WC, Møller NP.. (2002) Discovery and SAR of a novel selective and orally bioavailable nonpeptide classical competitive inhibitor class of protein-tyrosine phosphatase 1B.. J Med Chem, 45 (20): (4443-4459). [PMID:12238924] [10.1021/jm0209026]
3. He Y, Liu S, Menon A, Stanford S, Oppong E, Gunawan AM, Wu L, Wu DJ, Barrios AM, Bottini N et al.. (2013) A potent and selective small-molecule inhibitor for the lymphoid-specific tyrosine phosphatase (LYP), a target associated with autoimmune diseases.. J Med Chem, 56 (12): (4990-5008). [PMID:23713581] [10.1021/op500134e]
4. Yu X, Sun JP, He Y, Guo X, Liu S, Zhou B, Hudmon A, Zhang ZY. (2007) Structure, inhibitor, and regulatory mechanism of Lyp, a lymphoid-specific tyrosine phosphatase implicated in autoimmune diseases.. Proc Natl Acad Sci USA, 104 (50): (19767-72). [PMID:18056643] [10.1021/op500134e]
5. Stanford SM, Krishnamurthy D, Falk MD, Messina R, Debnath B, Li S, Liu T, Kazemi R, Dahl R, He Y et al.. (2011) Discovery of a novel series of inhibitors of lymphoid tyrosine phosphatase with activity in human T cells.. J Med Chem, 54 (6): (1640-54). [PMID:21341673] [10.1021/op500134e]
6. Katherine A Rawls,P Therese Lang,Jun Takeuchi,Shinichi Imamura,Tyler D Baguley,Christoph Grundner,Tom Alber,Jonathan A Ellman. (2009-11-06) Fragment-based discovery of selective inhibitors of the Mycobacterium tuberculosis protein tyrosine phosphatase PtpA.. Bioorganic & medicinal chemistry letters, 19 ((24)): (6851-6854). [PMID:19889539]
7. Dufresne C, Roy P, Wang Z, Asante-Appiah E, Cromlish W, Boie Y, Forghani F, Desmarais S, Wang Q, Skorey K, Waddleton D, Ramachandran C, Kennedy BP, Xu L, Gordon R, Chan CC, Leblanc Y.. (2004) The development of potent non-peptidic PTP-1B inhibitors.. Bioorg Med Chem Lett, 14 (4): (1039-1042). [PMID:15013019] [10.1016/j.bmcl.2003.11.048]
8. Wilson DP, Wan ZK, Xu WX, Kirincich SJ, Follows BC, Joseph-McCarthy D, Foreman K, Moretto A, Wu J, Zhu M, Binnun E, Zhang YL, Tam M, Erbe DV, Tobin J, Xu X, Leung L, Shilling A, Tam SY, Mansour TS, Lee J.. (2007) Structure-based optimization of protein tyrosine phosphatase 1B inhibitors: from the active site to the second phosphotyrosine binding site.. J Med Chem, 50 (19): (4681-4698). [PMID:17705360] [10.1021/jm0702478]
9. Han Y, Belley M, Bayly CI, Colucci J, Dufresne C, Giroux A, Lau CK, Leblanc Y, McKay D, Therien M, Wilson MC, Skorey K, Chan CC, Scapin G, Kennedy BP.. (2008) Discovery of [(3-bromo-7-cyano-2-naphthyl)(difluoro)methyl]phosphonic acid, a potent and orally active small molecule PTP1B inhibitor.. Bioorg Med Chem Lett, 18 (11): (3200-3205). [PMID:18477508] [10.1016/j.bmcl.2008.04.064]
10. Cosconati S, Hong JA, Novellino E, Carroll KS, Goodsell DS, Olson AJ.. (2008) Structure-based virtual screening and biological evaluation of Mycobacterium tuberculosis adenosine 5'-phosphosulfate reductase inhibitors.. J Med Chem, 51 (21): (6627-6630). [PMID:18855373] [10.1021/jm800571m]
11. Yu WM, Guvench O, Mackerell AD, Qu CK.. (2008) Identification of small molecular weight inhibitors of Src homology 2 domain-containing tyrosine phosphatase 2 (SHP-2) via in silico database screening combined with experimental assay.. J Med Chem, 51 (23): (7396-7404). [PMID:19007293] [10.1021/jm800229d]
12. Vidović D, Schürer SC.. (2009) Knowledge-based characterization of similarity relationships in the human protein-tyrosine phosphatase family for rational inhibitor design.. J Med Chem, 52 (21): (6649-6659). [PMID:19810703] [10.1021/jm9008899]
13. Wu S, Vossius S, Rahmouni S, Miletic AV, Vang T, Vazquez-Rodriguez J, Cerignoli F, Arimura Y, Williams S, Hayes T, Moutschen M, Vasile S, Pellecchia M, Mustelin T, Tautz L.. (2009) Multidentate small-molecule inhibitors of vaccinia H1-related (VHR) phosphatase decrease proliferation of cervix cancer cells.. J Med Chem, 52 (21): (6716-6723). [PMID:19888758] [10.1021/jm901016k]
14. Zhang X, He Y, Liu S, Yu Z, Jiang ZX, Yang Z, Dong Y, Nabinger SC, Wu L, Gunawan AM, Wang L, Chan RJ, Zhang ZY.. (2010) Salicylic acid based small molecule inhibitor for the oncogenic Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2).. J Med Chem, 53 (6): (2482-2493). [PMID:20170098] [10.1021/jm901645u]
15. Vang T, Xie Y, Liu WH, Vidović D, Liu Y, Wu S, Smith DH, Rinderspacher A, Chung C, Gong G, Mustelin T, Landry DW, Rickert RC, Schürer SC, Deng SX, Tautz L.. (2011) Inhibition of lymphoid tyrosine phosphatase by benzofuran salicylic acids.. J Med Chem, 54 (2): (562-571). [PMID:21190368] [10.1021/jm101004d]
16. Yu ZH, Chen L, Wu L, Liu S, Wang L, Zhang ZY.. (2011) Small molecule inhibitors of SHP2 tyrosine phosphatase discovered by virtual screening.. Bioorg Med Chem Lett, 21 (14): (4238-4242). [PMID:21669525] [10.1016/j.bmcl.2011.05.078]
17. He Y, Xu J, Yu ZH, Gunawan AM, Wu L, Wang L, Zhang ZY.. (2013) Discovery and evaluation of novel inhibitors of mycobacterium protein tyrosine phosphatase B from the 6-Hydroxy-benzofuran-5-carboxylic acid scaffold.. J Med Chem, 56 (3): (832-842). [PMID:23305444] [10.1021/jm301781p]
18. Liu W, Yu B, Xu G, Xu WR, Loh ML, Tang LD, Qu CK.. (2013) Identification of cryptotanshinone as an inhibitor of oncogenic protein tyrosine phosphatase SHP2 (PTPN11).. J Med Chem, 56 (18): (7212-7221). [PMID:23957426] [10.1021/jm400474r]
19. Baguley TD, Xu HC, Chatterjee M, Nairn AC, Lombroso PJ, Ellman JA.. (2013) Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.. J Med Chem, 56 (19): (7636-7650). [PMID:24083656] [10.1021/jm401037h]
20. Tautz L, Senis YA, Oury C, Rahmouni S.. (2015) Perspective: Tyrosine phosphatases as novel targets for antiplatelet therapy.. Bioorg Med Chem, 23 (12): (2786-2797). [PMID:25921264] [10.1016/j.bmc.2015.03.075]
21. He R, Bai Y, Yu ZH, Wu L, Gunawan AM, Zhang ZY.. (2014) Diversity-Oriented Synthesis for Novel, Selective and Drug-like Inhibitors for a Phosphatase from Mycobacterium Tuberculosis.. Medchemcomm, 5 (10): (1496-1499). [PMID:25505942] [10.1039/c4md00099d]
22. Haftchenary S, Jouk AO, Aubry I, Lewis AM, Landry M, Ball DP, Shouksmith AE, Collins CV, Tremblay ML, Gunning PT.. (2015) Identification of Bidentate Salicylic Acid Inhibitors of PTP1B.. ACS Med Chem Lett, 6 (9): (982-986). [PMID:26396684] [10.1021/acsmedchemlett.5b00171]
23. Li Y, Xia G, Guo Q, Wu L, Chen S, Yang Z, Wang W, Zhang ZY, Zhou X, Jiang ZX.. (2016) Design, synthesis and evaluation of novel ¹⁹F magnetic resonance sensitive protein tyrosine phosphatase inhibitors.. Medchemcomm, 7 (8): (1672-1680). [PMID:27529021] [10.1039/C6MD00277C]
24. Abdjul DB, Yamazaki H, Kanno SI, Wewengkang DS, Rotinsulu H, Sumilat DA, Ukai K, Kapojos MM, Namikoshi M.. (2017) Furanoterpenes, new types of protein tyrosine phosphatase 1B inhibitors, from two Indonesian marine sponges, Ircinia and Spongia spp.. Bioorg Med Chem Lett, 27 (5): (1159-1161). [PMID:28189419] [10.1016/j.bmcl.2017.01.071]
25. He R,Wang J,Yu ZH,Zhang RY,Liu S,Wu L,Zhang ZY. (2016) Inhibition of Low Molecular Weight Protein Tyrosine Phosphatase by an Induced-Fit Mechanism.. J Med Chem, 59 (19.0): (9094-9106). [PMID:27676368] [10.1021/acs.jmedchem.6b00993]
26. Streuli, M M, Krueger, N X NX, Thai, T T, Tang, M M and Saito, H H.. (1990) Distinct functional roles of the two intracellular phosphatase like domains of the receptor-linked protein tyrosine phosphatases LCA and LAR.. The EMBO journal, [PMID:1695146]
27. Tsai, A Y AY, Streuli, M M and Saito, H H.. (1989) Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.. Molecular and cellular biology, [PMID:2531281]
28. Streuli, M M, Hall, L R LR, Saga, Y Y, Schlossman, S F SF and Saito, H H.. (1987) Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.. The Journal of experimental medicine, (1): [PMID:2824653]
29. Charbonneau, H H, Tonks, N K NK, Walsh, K A KA and Fischer, E H EH.. (1988) The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2845400]
30. Ralph, S J SJ, Thomas, M L ML, Morton, C C CC and Trowbridge, I S IS.. (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen).. The EMBO journal, [PMID:2956090]
31. Hall, L R LR, Streuli, M M, Schlossman, S F SF and Saito, H H.. (1988) Complete exon-intron organization of the human leukocyte common antigen (CD45) gene.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2971730]
32. Kung, C C and 12 more authors.. (2000) Mutations in the tyrosine phosphatase CD45 gene in a child with severe combined immunodeficiency disease.. Nature medicine, [PMID:10700239]
33. Jacobsen, M M and 15 more authors.. (2000) A point mutation in PTPRC is associated with the development of multiple sclerosis.. Nature genetics, [PMID:11101853]
34. Tchilian, E Z EZ and 5 more authors.. (2001) A deletion in the gene encoding the CD45 antigen in a patient with SCID.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11145714]
35. Wu, Liangtang L, Fu, Jun J and Shen, Shi-Hsiang SH.. (2002) SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription.. Molecular and cellular biology, [PMID:11909961]
36. Fukuhara, Kenjiro K and 6 more authors.. (2002) A study on CD45 isoform expression during T-cell development and selection events in the human thymus.. Human immunology, [PMID:11975983]
37. Nam, Hyun-Joo HJ, Poy, Florence F, Saito, Haruo H and Frederick, Christin A CA.. (2005) Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45.. The Journal of experimental medicine, (7): [PMID:15684325]
38. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
39. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
40. Panchal, Rekha G RG and 10 more authors.. (2009) Reduced expression of CD45 protein-tyrosine phosphatase provides protection against anthrax pathogenesis.. The Journal of biological chemistry, (8): [PMID:19269962]
41. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
42. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
43. Gabaev, Ildar I and 9 more authors.. (2011) The human cytomegalovirus UL11 protein interacts with the receptor tyrosine phosphatase CD45, resulting in functional paralysis of T cells.. PLoS pathogens, [PMID:22174689]
44. Roberts, Joseph L JL and 12 more authors.. (2012) CD45-deficient severe combined immunodeficiency caused by uniparental disomy.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:22689986]
45. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
46. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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