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Ab006750-10μg

10μg

Available within 8-12 weeks(?)

$16.90

Ab006750-50μg

50μg

In stock

$39.90

Ab006750-100μg

100μg

In stock

$69.90

Ab006750-1mg

1mg

Available within 8-12 weeks(?)

$619.90

Basic Description

Product Name	CD45RA Mouse mAb - Primary antibody, specific to CD45RA, Mouse IgG2b
Synonyms	B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotei
Specifications & Purity	ExactAb™, Validated, Carrier Free, Lot by Lot
Host species	Mouse
Specificity	CD45RA
Immunogen	Low buoyancy density human lymphocytes
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD45RA Antibody, Monoclonal (HI100), could be used for Flow and so on. Application: Flow: < 0.5 μg/100w cells Protein Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

Product Properties

Isotype	Mouse IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD45RA Mouse mAb (Ab006750) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45RA with CD45RA Mouse mAb (Ab006750) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

CD45RA Mouse mAb (Ab006750) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45RA (red) with CD45RA Mouse mAb (Ab006750) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

CD45RA Mouse mAb (Ab006750) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD45RA (red) with CD45RA Mouse mAb (Ab006750) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PTPRC Tchem Leukocyte common antigen (2317 Activities)

Discover Target: PTPRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Application	Dilution info
Flow Cytometry	< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1201950	Certificate of Analysis	Dec 06, 2023	Ab006750
ZJ23F1201951	Certificate of Analysis	Dec 06, 2023	Ab006750

FAQs and Articles

FAQ

Flow cytometry cell cycle analysis using propidium iodide DNA staining

Categories: Protocols

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Propidium Iodide Flow Cytometry Antibody Cell Cycle

Experimental Guide of Sample Preparation for Flow Cytometry

Categories: Protocols

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Protein sample preparation Flow Cytometry Antibody FACS

References

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CD45RA Mouse mAb - Primary antibody, specific to CD45RA, Mouse IgG2b