| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab094961-10μg | 10μg | In stock | $26.90 | |
| Ab094961-50μg | 50μg | In stock | $69.90 | |
| Ab094961-100μg | 100μg | In stock | $109.90 | |
| Ab094961-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (CC2C6); Mouse anti Human CD47 Antibody; Flow; Unconjugated
| Product Name | CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1 |
|---|---|
| Synonyms | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein | |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD47 |
| Immunogen | CCRF-CEM T-cell line. |
| Positive Control | Flow: Human peripheral blood lymphocytes. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD47 Antibody, Monoclonal (CC2C6), could be used for Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD47 (red) with CD47 Mouse mAb (Ab094961) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094961) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094961) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 with CD47 Mouse mAb (Ab094961) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD47 Mouse mAb (Ab094961) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 (red) with CD47 Mouse mAb (Ab094961) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1μg/100w cells "> 1μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404669 | Certificate of Analysis | Apr 19, 2024 | Ab094961 |
| ZJ24F0404668 | Certificate of Analysis | Apr 19, 2024 | Ab094961 |
| ZJ24F0404667 | Certificate of Analysis | Apr 19, 2024 | Ab094961 |
| 1. Kauder SE, Kuo TC, Harrabi O, Chen A, Sangalang E, Doyle L, Rocha SS, Bollini S, Han B, Sim J et al.. (2018) ALX148 blocks CD47 and enhances innate and adaptive antitumor immunity with a favorable safety profile.. PLoS One, 13 (8): (e0201832). [PMID:30133535] [10.1021/op500134e] |
| 2. Petrova PS, Viller NN, Wong M, Pang X, Lin GH, Dodge K, Chai V, Chen H, Lee V, House V et al.. (2017) TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.. Clin Cancer Res, 23 (4): (1068-1079). [PMID:27856600] [10.1021/op500134e] |
| 3. Campbell, I G IG, Freemont, P S PS, Foulkes, W W and Trowsdale, J J.. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains.. Cancer research, (1): [PMID:1394148] |
| 4. Lindberg, F P FP, Gresham, H D HD, Schwarz, E E and Brown, E J EJ.. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding.. The Journal of cell biology, [PMID:7691831] |
| 5. Mawby, W J WJ, Holmes, C H CH, Anstee, D J DJ, Spring, F A FA and Tanner, M J MJ.. (1994) Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3.. The Biochemical journal, (1): [PMID:7998989] |
| 6. Gao, A G AG and 5 more authors.. (1996) Integrin-associated protein is a receptor for the C-terminal domain of thrombospondin.. The Journal of biological chemistry, (5): [PMID:8550562] |
| 7. Wu, A L AL, Wang, J J, Zheleznyak, A A and Brown, E J EJ.. (1999) Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane.. Molecular cell, [PMID:10549293] |
| 8. Rebres, R A RA, Vaz, L E LE, Green, J M JM and Brown, E J EJ.. (2001) Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains.. The Journal of biological chemistry, (14): [PMID:11454874] |
| 9. Latour, S S and 11 more authors.. (2001) Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11509594] |
| 10. Piccio, Laura L and 7 more authors.. (2005) Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation.. Blood, (15): [PMID:15383453] |
| 11. Manna, Partha P PP, Dimitry, Julie J, Oldenborg, Per-Arne PA and Frazier, William A WA.. (2005) CD47 augments Fas/CD95-mediated apoptosis.. The Journal of biological chemistry, (19): [PMID:15917238] |
| 12. Hatherley, Deborah D and 5 more authors.. (2008) Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.. Molecular cell, (25): [PMID:18657508] |
| 13. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
| 14. De Oliveira, S S, Vitorino de Almeida, V V, Calado, A A, Rosário, H S HS and Saldanha, C C.. (2012) Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane.. Biochimica et biophysica acta, [PMID:22079249] |
| 15. Rogers, Natasha M NM and 24 more authors.. (2017) TSP1-CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction.. Cardiovascular research, [PMID:27742621] |
| 16. Ghimire, Kedar and 11 more authors.. (2020) CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis.. Cells, (15): [PMID:32679764] |
