CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: B6H12
  • Conjugation: Unconjugated
Item Number
Ab094962
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab094962-10μg
10μg
In stock
$26.90
Ab094962-50μg
50μg
In stock
$69.90
Ab094962-100μg
100μg
In stock
$109.90
Ab094962-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$899.90

mAb (B6H12); Mouse anti Human CD47 Antibody; Flow; Unconjugated

Basic Description

Product NameCD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1
Synonymsantigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein |
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host speciesMouse
SpecificityCD47
ImmunogenPurified human placental RGD-binding proteins
Positive ControlFlow: Human peripheral blood lymphocytes.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD47 Antibody, Monoclonal (B6H12), could be used for Flow and so on.

Application:

Flow: 1.0 μg/100w cells

Protein Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Associated Targets(Human)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry1μg/100w cells

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1μg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505964Certificate of AnalysisMay 22, 2024 Ab094962
ZJ24F0505965Certificate of AnalysisMay 22, 2024 Ab094962
ZJ24F0505966Certificate of AnalysisMay 22, 2024 Ab094962

Related Documents

References

1. Kauder SE, Kuo TC, Harrabi O, Chen A, Sangalang E, Doyle L, Rocha SS, Bollini S, Han B, Sim J et al..  (2018)  ALX148 blocks CD47 and enhances innate and adaptive antitumor immunity with a favorable safety profile..  PLoS One,  13  (8): (e0201832).  [PMID:30133535] [10.1021/op500134e]
2. Petrova PS, Viller NN, Wong M, Pang X, Lin GH, Dodge K, Chai V, Chen H, Lee V, House V et al..  (2017)  TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding..  Clin Cancer Res,  23  (4): (1068-1079).  [PMID:27856600] [10.1021/op500134e]
3. Campbell, I G IG, Freemont, P S PS, Foulkes, W W and Trowsdale, J J..  (1992)  An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains..  Cancer research,    (1):   [PMID:1394148]
4. Lindberg, F P FP, Gresham, H D HD, Schwarz, E E and Brown, E J EJ..  (1993)  Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding..  The Journal of cell biology,      [PMID:7691831]
5. Mawby, W J WJ, Holmes, C H CH, Anstee, D J DJ, Spring, F A FA and Tanner, M J MJ..  (1994)  Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3..  The Biochemical journal,    (1):   [PMID:7998989]
6. Gao, A G AG and 5 more authors..  (1996)  Integrin-associated protein is a receptor for the C-terminal domain of thrombospondin..  The Journal of biological chemistry,    (5):   [PMID:8550562]
7. Wu, A L AL, Wang, J J, Zheleznyak, A A and Brown, E J EJ..  (1999)  Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane..  Molecular cell,      [PMID:10549293]
8. Rebres, R A RA, Vaz, L E LE, Green, J M JM and Brown, E J EJ..  (2001)  Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains..  The Journal of biological chemistry,    (14):   [PMID:11454874]
9. Latour, S S and 11 more authors..  (2001)  Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:11509594]
10. Piccio, Laura L and 7 more authors..  (2005)  Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation..  Blood,    (15):   [PMID:15383453]
11. Manna, Partha P PP, Dimitry, Julie J, Oldenborg, Per-Arne PA and Frazier, William A WA..  (2005)  CD47 augments Fas/CD95-mediated apoptosis..  The Journal of biological chemistry,    (19):   [PMID:15917238]
12. Hatherley, Deborah D and 5 more authors..  (2008)  Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47..  Molecular cell,    (25):   [PMID:18657508]
13. Wollscheid, Bernd B and 7 more authors..  (2009)  Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins..  Nature biotechnology,      [PMID:19349973]
14. De Oliveira, S S, Vitorino de Almeida, V V, Calado, A A, Rosário, H S HS and Saldanha, C C..  (2012)  Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane..  Biochimica et biophysica acta,      [PMID:22079249]
15. Rogers, Natasha M NM and 24 more authors..  (2017)  TSP1-CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction..  Cardiovascular research,      [PMID:27742621]
16. Ghimire, Kedar and 11 more authors..  (2020)  CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis..  Cells,    (15):   [PMID:32679764]

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