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Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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SKU | Size | Availability | Price | Qty |
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Ab094962-10μg | 10μg | In stock | $26.90 | |
Ab094962-50μg | 50μg | In stock | $69.90 | |
Ab094962-100μg | 100μg | In stock | $109.90 | |
Ab094962-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (B6H12); Mouse anti Human CD47 Antibody; Flow; Unconjugated
Product Name | CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1 |
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Synonyms | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein | |
Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
Host species | Mouse |
Specificity | CD47 |
Immunogen | Purified human placental RGD-binding proteins |
Positive Control | Flow: Human peripheral blood lymphocytes. |
Conjugation | Unconjugated |
Grade | Azide Free, Carrier Free, ExactAb™, Validated |
Product Description | Mouse anti Human CD47 Antibody, Monoclonal (B6H12), could be used for Flow and so on. Application: Flow: 1.0 μg/100w cells Protein Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. |
Isotype | Mouse IgG1 |
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Light Chain Type | kappa |
SDS-PAGE | 150 kDa |
Purification Method | Protein G purified |
Form | Liquid |
Concentration | Lot by Lot |
Storage Temp | Store at 2-8°C |
Shipped In | Wet ice |
Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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Application | Dilution info |
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Flow Cytometry | 1μg/100w cells "> 1μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
Lot Number | Certificate Type | Date | Item |
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ZJ24F0505964 | Certificate of Analysis | May 22, 2024 | Ab094962 |
ZJ24F0505965 | Certificate of Analysis | May 22, 2024 | Ab094962 |
ZJ24F0505966 | Certificate of Analysis | May 22, 2024 | Ab094962 |
1. Kauder SE, Kuo TC, Harrabi O, Chen A, Sangalang E, Doyle L, Rocha SS, Bollini S, Han B, Sim J et al.. (2018) ALX148 blocks CD47 and enhances innate and adaptive antitumor immunity with a favorable safety profile.. PLoS One, 13 (8): (e0201832). [PMID:30133535] [10.1021/op500134e] |
2. Petrova PS, Viller NN, Wong M, Pang X, Lin GH, Dodge K, Chai V, Chen H, Lee V, House V et al.. (2017) TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.. Clin Cancer Res, 23 (4): (1068-1079). [PMID:27856600] [10.1021/op500134e] |
3. Campbell, I G IG, Freemont, P S PS, Foulkes, W W and Trowsdale, J J.. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains.. Cancer research, (1): [PMID:1394148] |
4. Lindberg, F P FP, Gresham, H D HD, Schwarz, E E and Brown, E J EJ.. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding.. The Journal of cell biology, [PMID:7691831] |
5. Mawby, W J WJ, Holmes, C H CH, Anstee, D J DJ, Spring, F A FA and Tanner, M J MJ.. (1994) Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3.. The Biochemical journal, (1): [PMID:7998989] |
6. Gao, A G AG and 5 more authors.. (1996) Integrin-associated protein is a receptor for the C-terminal domain of thrombospondin.. The Journal of biological chemistry, (5): [PMID:8550562] |
7. Wu, A L AL, Wang, J J, Zheleznyak, A A and Brown, E J EJ.. (1999) Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane.. Molecular cell, [PMID:10549293] |
8. Rebres, R A RA, Vaz, L E LE, Green, J M JM and Brown, E J EJ.. (2001) Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains.. The Journal of biological chemistry, (14): [PMID:11454874] |
9. Latour, S S and 11 more authors.. (2001) Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11509594] |
10. Piccio, Laura L and 7 more authors.. (2005) Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation.. Blood, (15): [PMID:15383453] |
11. Manna, Partha P PP, Dimitry, Julie J, Oldenborg, Per-Arne PA and Frazier, William A WA.. (2005) CD47 augments Fas/CD95-mediated apoptosis.. The Journal of biological chemistry, (19): [PMID:15917238] |
12. Hatherley, Deborah D and 5 more authors.. (2008) Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.. Molecular cell, (25): [PMID:18657508] |
13. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
14. De Oliveira, S S, Vitorino de Almeida, V V, Calado, A A, Rosário, H S HS and Saldanha, C C.. (2012) Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane.. Biochimica et biophysica acta, [PMID:22079249] |
15. Rogers, Natasha M NM and 24 more authors.. (2017) TSP1-CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction.. Cardiovascular research, [PMID:27742621] |
16. Ghimire, Kedar and 11 more authors.. (2020) CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis.. Cells, (15): [PMID:32679764] |