| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab190282-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $58.90 | |
| Ab190282-25μg | 25μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $117.90 | |
| Ab190282-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $249.90 |
mAb (MIAP301); Rat anti Mouse CD47 Antibody (AF647); Flow
| Product Name | CD47 Rat mAb (AF647) |
|---|---|
| Synonyms | Itgp | 9130415E20Rik | B430305P08Rik | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | |
| Specifications & Purity | ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, bind |
| Host species | Rat |
| Specificity | CD47 |
| Immunogen | Intact CD47 purified from placenta |
| Positive Control | Flow: C57BL/6 mouse splenocytes. |
| Conjugation | Alexa Fluor® 647 |
| Grade | Em:668nm, Ex:650nm, ExactAb™, Validated |
| Product Description | Rat anti Mouse CD47 Antibody, Monoclonal (MIAP301), could be used for Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC and so on. |
| Isotype | Rat IgG2a |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
CD47 Rat mAb (AF647) (Ab190282) - Flow Cytometry
Flow cytometry analysis of CD47 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD47 Rat mAb (AF647) (Ab190282) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry |
≤1.0 μg/test,
the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume
|
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