| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab006495-10μg | 10μg | In stock | $26.90 | |
| Ab006495-50μg | 50μg | In stock | $69.90 | |
| Ab006495-100μg | 100μg | In stock | $99.90 | |
| Ab006495-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (156-4H9); Mouse anti Human CD48 Antibody; Flow; Unconjugated
| Product Name | CD48 Mouse mAb - Primary antibody, specific to CD48, Mouse IgG1 |
|---|---|
| Synonyms | BCM1 surface antigen | BCM1 | BCM1Leukocyte antigen MEM-102 | BLAST | BLAST1 | BLAST1TCT.1 | B-lymphocyte activation marker BLAST-1 | CD48 antigen (B-cell membrane protein) | CD48 antigen | CD48 molecule | CD48 | hCD48 | mCD48 | MEM-102 | SLAMF2 | Antigen |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD48 |
| Positive Control | Flow: Human peripheral blood lymphocytes. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD48 Antibody, Monoclonal (156-4H9), could be used for Flow and so on. Application Flow: 0.25 μg/100w cells Protein Function Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
CD48 Mouse mAb (Ab006495) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD48 (red) with CD48 Mouse mAb (Ab006495) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD48 Mouse mAb (Ab006495) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD48 (red) with CD48 Mouse mAb (Ab006495) at 5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD48 Mouse mAb (Ab006495) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD48 with CD48 Mouse mAb (Ab006495) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD48 Mouse mAb (Ab006495) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD48 (red) with CD48 Mouse mAb (Ab006495) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 0.25μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404657 | Certificate of Analysis | Apr 19, 2024 | Ab006495 |
| ZJ24F0404656 | Certificate of Analysis | Apr 19, 2024 | Ab006495 |
| ZJ24F0404655 | Certificate of Analysis | Apr 19, 2024 | Ab006495 |
| 1. Del Porto, P P and 6 more authors.. (1991) TCT.1, a target molecule for gamma/delta T cells, is encoded by an immunoglobulin superfamily gene (Blast-1) located in the CD1 region of human chromosome 1.. The Journal of experimental medicine, (1): [PMID:1827826] |
| 2. Fisher, R C RC and Thorley-Lawson, D A DA.. (1991) Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48).. Molecular and cellular biology, [PMID:1847502] |
| 3. Korínek, V V, Stefanová, I I, Angelisová, P P, Hilgert, I I and Horejsí, V V.. (1991) The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.. Immunogenetics, [PMID:1999350] |
| 4. Vaughan, H A HA, Henning, M M MM, Purcell, D F DF, McKenzie, I F IF and Sandrin, M S MS.. (1991) The isolation of cDNA clones for CD48.. Immunogenetics, [PMID:1999351] |
| 5. Staunton, D E DE and Thorley-Lawson, D A DA.. (1987) Molecular cloning of the lymphocyte activation marker Blast-1.. The EMBO journal, (1): [PMID:2828034] |
| 6. Smith, G M GM, Biggs, J J, Norris, B B, Anderson-Stewart, P P and Ward, R R.. (1997) Detection of a soluble form of the leukocyte surface antigen CD48 in plasma and its elevation in patients with lymphoid leukemias and arthritis.. Journal of clinical immunology, [PMID:9418191] |
| 7. Brown, M H MH and 5 more authors.. (1998) 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.. The Journal of experimental medicine, (7): [PMID:9841922] |
| 8. Katsuura, M M and 8 more authors.. (1998) CD48 expression on leukocytes in infectious diseases: flow cytometric analysis of surface antigen.. Acta paediatrica Japonica : Overseas edition, [PMID:9893294] |
| 9. Hawash, Ibrahim Y IY and 5 more authors.. (2002) The oxygen-substituted palmitic acid analogue, 13-oxypalmitic acid, inhibits Lck localization to lipid rafts and T cell signaling.. Biochimica et biophysica acta, (3): [PMID:12007789] |
| 10. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 11. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
| 12. Muhammad, Arshad A and 10 more authors.. (2009) Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:19494291] |
| 13. Claus, Maren M, Wingert, Sabine S and Watzl, Carsten C.. (2016) Modulation of natural killer cell functions by interactions between 2B4 and CD48 in cis and in trans.. Open biology, [PMID:27249817] |
