CD5 Mouse mAb - Primary antibody, specific to CD5, Mouse IgG1

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: UCHT-2
  • Conjugation: Unconjugated
Item Number
Ab006673
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab006673-10μg
10μg
In stock
$16.90
Ab006673-50μg
50μg
In stock
$49.90
Ab006673-100μg
100μg
In stock
$89.90
Ab006673-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$799.90

mAb (UCHT-2); Mouse anti Human CD5 Antibody; Flow; Unconjugated

Basic Description

Product NameCD5 Mouse mAb - Primary antibody, specific to CD5, Mouse IgG1
SynonymsCD5 antigen (p56-62) | CD5 antigen | CD5 molecule | CD5 | LEU1T-cell surface glycoprotein CD5 | Lymphocyte antigen T1/Leu-1 | T1 | CD 5 antibody | CD5 antibody | CD5 antigen (p56 62) antibody | CD5 antigen antibody | CD5 molecule antibody | CD5_HUMAN anti
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host speciesMouse
SpecificityCD5
Positive ControlFlow: Human peripheral blood lymphocytes.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD5 Antibody, Monoclonal (UCHT-2), could be used for Flow and so on.
Application:
Flow: 0.25 μg/100w cells
Protein Function
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Images

CD5 Mouse mAb (Ab006673) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD5 (red) with CD5 Mouse mAb (Ab006673) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD5 Mouse mAb (Ab006673) - Flow Cytometry
Flow Cytometry analysis of MOLT-4 cells labelling CD5 (red) with CD5 Mouse mAb (Ab006673) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD5 Mouse mAb (Ab006673) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD5 with CD5 Mouse mAb (Ab006673) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

CD5 Mouse mAb (Ab006673) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD5 (red) with CD5 Mouse mAb (Ab006673) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

CD5 Tbio T-cell surface glycoprotein CD5 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry

0.25μg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505145Certificate of AnalysisMay 07, 2024 Ab006673
ZJ24F0505146Certificate of AnalysisMay 07, 2024 Ab006673
ZJ24F0505144Certificate of AnalysisMay 07, 2024 Ab006673

Related Documents

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334] [10.1021/op500134e]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039] [10.1021/op500134e]
3. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki.  (2006-03-24)  Human chromosome 11 DNA sequence and analysis including novel gene identification..  Nature,  440  ((7083)): (497-500).  [PMID:16554811]
4. Van de Velde, H H, von Hoegen, I I, Luo, W W, Parnes, J R JR and Thielemans, K K..  (1991)  The B-cell surface protein CD72/Lyb-2 is the ligand for CD5..  Nature,    (20):   [PMID:1711157]
5. Jones, N H NH and 5 more authors..  (1986)  Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1..  Nature,      [PMID:3093892]
6. Salomon, Arthur R AR and 10 more authors..  (2003)  Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry..  Proceedings of the National Academy of Sciences of the United States of America,    (21):   [PMID:12522270]
7. Brill, Laurence M LM and 5 more authors..  (2004)  Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry..  Analytical chemistry,    (15):   [PMID:15144186]
8. Garza-Garcia, Acely A and 6 more authors..  (2008)  Three-dimensional solution structure and conformational plasticity of the N-terminal scavenger receptor cysteine-rich domain of human CD5..  Journal of molecular biology,    (18):   [PMID:18339402]
9. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]

Solution Calculators