Skip to the beginning of the images gallery

CD57 Mouse mAb - Primary antibody, specific to CD57, Mouse IgM

Lot by Lot

Application:

Flow Cytometry

Features and benefits

Host species: Mouse
Species reactivity(Reacts with): Human
Isotype: Mouse IgM
Clone Number: HNK-1
Conjugation: Unconjugated

Item Number

Ab006583

Grouped product items
SKU	Size	Availability	Price
Ab006583-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$26.90
Ab006583-50μg	50μg	In stock	$79.90
Ab006583-100μg	100μg	In stock	$129.90
Ab006583-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,039.90

mAb (HNK-1); Mouse anti Human CD57 Antibody; Flow; Unconjugated

View related series

Click to view target information: B3GAT1

Accession#:Q9P2W7 B3GAT1 CD57 Gene ID:27087

Basic Description

Product Name	CD57 Mouse mAb - Primary antibody, specific to CD57, Mouse IgM
Synonyms	Beta-1,3-glucuronyltransferase 1 \| EC 2.4.1.135 \| GlcAT-P \| GlcUAT-P \| Glucuronosyltransferase P \| UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Mouse
Specificity	CD57
Immunogen	Membrane extract of human lymphoblastoid cell line HSB-2.
Positive Control	Flow: Human peripheral blood lymphocytes.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD57 Antibody, Monoclonal (HNK-1), could be used for Flow and so on. Application: Flow: 0.125μg/100w cells Protein Function Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Isotype

Mouse IgM

Purification Method

Protein G purified

Form

Liquid

Concentration

Lot by Lot

Storage Temp

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Images

CD57 Mouse mAb (Ab006583) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD57 with CD57 Mouse mAb (Ab006583) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

CD57 Mouse mAb (Ab006583) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD57 (red) with CD57 Mouse mAb (Ab006583) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Application

Dilution info

Flow Cytometry

0.125µg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1101543	Certificate of Analysis	Jul 30, 2024	Ab006583
ZJ23F1101544	Certificate of Analysis	Jul 30, 2024	Ab006583

References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039] [10.1021/op500134e]
2. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
3. Mitsumoto, Y Y, Oka, S S, Sakuma, H H, Inazawa, J J and Kawasaki, T T.. (2000) Cloning and chromosomal mapping of human glucuronyltransferase involved in biosynthesis of the HNK-1 carbohydrate epitope.. Genomics, (15): [PMID:10783264]
4. Kakuda, Shinako S and 8 more authors.. (2004) Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1.. The Journal of biological chemistry, (21): [PMID:14993226]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.