CD79a Antibody - Primary antibody, specific to CD79a, Rabbit IgG

Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human CD79a Antibody; WB, IHC, Flow; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • Flow Cytometry
  • IHC
  • WB
Item Number
Ab095317
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab095317-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$26.90
Ab095317-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab095317-50μl
50μl
In stock
$79.90
Ab095317-100μl
100μl
In stock
$109.90
Ab095317-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$899.90

pAb; Rabbit anti Human CD79a Antibody; WB, IHC, Flow; Unconjugated

Basic Description

Product NameCD79a Antibody - Primary antibody, specific to CD79a, Rabbit IgG
SynonymsB lymphocyte-specific MB1 protein antibody; B-cell antigen receptor complex-associated protein alpha chain antibody; CD 79a antibody; CD79a antibody; CD79a antigen (immunoglobulin-associated alpha) antibody; CD79A antigen antibody; CD79a molecule, immunog
Specifications & PurityExactAb™, Validated, Carrier Free, Lot by Lot
Host speciesRabbit
SpecificityCD79a
ImmunogenRecombinant CD79a expressed in E.coli (AA 36-219)
Positive ControlWB: Ramos and Raji cell lysates. IHC: Human lymphoma tissue. Flow: Human Raji Burkitt's lymphoma cell line.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human CD79a Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application
WB: 0.5-2 µg/mL
IHC: 5-20 µg/mL
Flow: 20μg/mL
Protein Function
Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified and protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

CD79a Antibody (Ab095317) - Western Blot
All lanes: CD79a Antibody (Ab095317) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 25 kDa
Observed band size: 45 kDa
Exposure time: 10.0 s

CD79a Antibody (Ab095317) - IHC
DAB staining on IHC
Samples: Human Lymphoma Tissue
Primary Ab: 10 µg/mL CD79a Antibody (Ab095317)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

CD79a Antibody (Ab095317) - Flow Cytometry
Raji human Burkitt‘s lymphoma cell line was stained with CD79a Antibody (Ab095317) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody.

Associated Targets(Human)

CD79A Tbio B-cell antigen receptor complex-associated protein alpha chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.5-2 µg/mL

Flow Cytometry

20μg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101594Certificate of AnalysisNov 08, 2023 Ab095317
ZJ23F1101595Certificate of AnalysisNov 08, 2023 Ab095317

Related Documents

References

1. Leduc, I I, Preud'homme, J L JL and Cogné, M M..  (1992)  Structure and expression of the mb-1 transcript in human lymphoid cells..  Clinical and experimental immunology,      [PMID:1395095]
2. Müller, B B, Cooper, L L and Terhorst, C C..  (1992)  Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29..  European journal of immunology,      [PMID:1534761]
3. Ha, H J HJ, Kubagawa, H H and Burrows, P D PD..  (1992)  Molecular cloning and expression pattern of a human gene homologous to the murine mb-1 gene..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:1538135]
4. Flaswinkel, H H and Reth, M M..  (1992)  Molecular cloning of the Ig-alpha subunit of the human B-cell antigen receptor complex..  Immunogenetics,      [PMID:1639443]
5. Yu, L M LM and Chang, T W TW..  (1992)  Human mb-1 gene: complete cDNA sequence and its expression in B cells bearing membrane Ig of various isotypes..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:1729378]
6. Sakaguchi, N N, Kashiwamura, S S, Kimoto, M M, Thalmann, P P and Melchers, F F..  (1988)  B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties..  The EMBO journal,      [PMID:2463161]
7. Vasile, S S, Coligan, J E JE, Yoshida, M M and Seon, B K BK..  (1994)  Isolation and chemical characterization of the human B29 and mb-1 proteins of the B cell antigen receptor complex..  Molecular immunology,      [PMID:7514267]
8. Hashimoto, S S, Chiorazzi, N N and Gregersen, P K PK..  (1995)  Alternative splicing of CD79a (Ig-alpha/mb-1) and CD79b (Ig-beta/B29) RNA transcripts in human B cells..  Molecular immunology,      [PMID:7643857]
9. Hashimoto, S S, Mohrenweiser, H W HW, Gregersen, P K PK and Chiorazzi, N N..  (1994)  Chromosomal localization, genomic structure, and allelic polymorphism of the human CD79 alpha (Ig-alpha/mb-1) gene..  Immunogenetics,      [PMID:7916003]
10. Ha, H H, Barnoski, B L BL, Sun, L L, Emanuel, B S BS and Burrows, P D PD..  (1994)  Structure, chromosomal localization, and methylation pattern of the human mb-1 gene..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:8207205]
11. Minegishi, Y Y and 5 more authors..  (1999)  Mutations in Igalpha (CD79a) result in a complete block in B-cell development..  The Journal of clinical investigation,      [PMID:10525050]
12. Gaul, B S BS, Harrison, M L ML, Geahlen, R L RL, Burton, R A RA and Post, C B CB..  (2000)  Substrate recognition by the Lyn protein-tyrosine kinase. NMR structure of the immunoreceptor tyrosine-based activation motif signaling region of the B cell antigen receptor..  The Journal of biological chemistry,    (26):   [PMID:10748115]
13. Wang, Yue Y and 6 more authors..  (2002)  Novel Igalpha (CD79a) gene mutation in a Turkish patient with B cell-deficient agammaglobulinemia..  American journal of medical genetics,    (1):   [PMID:11920841]

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