| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab095357-10μg | 10μg | In stock | $16.90 | |
| Ab095357-50μg | 50μg | In stock | $39.90 | |
| Ab095357-100μg | 100μg | In stock | $69.90 | |
| Ab095357-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $619.90 |
mAb (HIT8a); Mouse anti Human CD8 Antibody; Flow; Unconjugated
| Product Name | CD8 Mouse mAb - Primary antibody, specific to CD8, Mouse IgG1 |
|---|---|
| Synonyms | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell surface glycoprotein CD8 alph |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD8 |
| Immunogen | Tissue, cells or virus. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human CD8 Antibody, Monoclonal (HIT8a), could be used for Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot. |
CD8 Mouse mAb (Ab095357) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 with CD8 Mouse mAb (Ab095357) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD8 Mouse mAb (Ab095357) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 (red) with CD8 Mouse mAb (Ab095357) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | < 0.5µg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202883 | Certificate of Analysis | Mar 01, 2024 | Ab095357 |
| ZJ24F0202882 | Certificate of Analysis | Mar 01, 2024 | Ab095357 |
| ZJ24F0202881 | Certificate of Analysis | Mar 01, 2024 | Ab095357 |
| 1. Peter W Kim,Zhen-Yu J Sun,Stephen C Blacklow,Gerhard Wagner,Michael J Eck. (2003-09-23) A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8.. Science (New York, N.Y.), 301 ((5640)): (1725-1728). [PMID:14500983] |
| 2. Leahy, D J DJ, Axel, R R and Hendrickson, W A WA.. (1992) Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution.. Cell, (20): [PMID:1547508] |
| 3. Norment, A M AM, Lonberg, N N, Lacy, E E and Littman, D R DR.. (1989) Alternatively spliced mRNA encodes a secreted form of human CD8 alpha. Characterization of the human CD8 alpha gene.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2496167] |
| 4. Nakayama, K K, Tokito, S S, Okumura, K K and Nakauchi, H H.. (1989) Structure and expression of the gene encoding CD8 alpha chain (Leu-2/T8).. Immunogenetics, [PMID:2509342] |
| 5. Giblin, P P, Ledbetter, J A JA and Kavathas, P P.. (1989) A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2536941] |
| 6. Salter, R D RD and 6 more authors.. (1989) Polymorphism in the alpha 3 domain of HLA-A molecules affects binding to CD8.. Nature, (23): [PMID:2784196] |
| 7. Littman, D R DR, Thomas, Y Y, Maddon, P J PJ, Chess, L L and Axel, R R.. (1985) The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes.. Cell, [PMID:3871356] |
| 8. Sukhatme, V P VP, Sizer, K C KC, Vollmer, A C AC, Hunkapiller, T T and Parnes, J R JR.. (1985) The T cell differentiation antigen Leu-2/T8 is homologous to immunoglobulin and T cell receptor variable regions.. Cell, [PMID:3918796] |
| 9. Parnes, J R JR, Sizer, K C KC, Sukhatme, V P VP and Hunkapiller, T T.. (1985) Structure of Leu-2/T8 as deduced from the sequence of a cDNA clone.. Behring Institute Mitteilungen, [PMID:3936473] |
| 10. Snow, P M PM and Terhorst, C C.. (1983) The T8 antigen is a multimeric complex of two distinct subunits on human thymocytes but consists of homomultimeric forms on peripheral blood T lymphocytes.. The Journal of biological chemistry, (10): [PMID:6605969] |
| 11. Schlesinger, M M and 6 more authors.. (1994) A distinctive form of soluble CD8 is secreted by stimulated CD8+ cells in HIV-1-infected and high-risk individuals.. Clinical immunology and immunopathology, [PMID:7923932] |
| 12. Gao, G F GF and 8 more authors.. (1997) Crystal structure of the complex between human CD8alpha(alpha) and HLA-A2.. Nature, (5): [PMID:9177355] |
| 13. de la Calle-Martin, O O and 9 more authors.. (2001) Familial CD8 deficiency due to a mutation in the CD8 alpha gene.. The Journal of clinical investigation, [PMID:11435463] |
| 14. Shiroishi, Mitsunori M and 13 more authors.. (2003) Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:12853576] |
| 15. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 16. Pang, Dick John DJ, Hayday, Adrian C AC and Bijlmakers, Marie-José MJ.. (2007) CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers.. The Journal of biological chemistry, (4): [PMID:17341584] |
| 17. Gibbings, Derrick J DJ, Marcet-Palacios, Marcelo M, Sekar, Yokananth Y, Ng, Marcus C Y MC and Befus, A Dean AD.. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.. BMC immunology, (1): [PMID:17678538] |
| 18. Zhu, Jia and 11 more authors.. (2013) Immune surveillance by CD8αα+ skin-resident T cells in human herpes virus infection.. Nature, (23): [PMID:23657257] |
| 19. Schuster, Philipp; Thomann, Sabrina; Werner, Maren; Vollmer, Jörg and Schmidt, Barbara.. (2015) A subset of human plasmacytoid dendritic cells expresses CD8α upon exposure to herpes simplex virus type 1.. Frontiers in microbiology, [PMID:26082771] |
