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CD9 Mouse mAb (Biotin) - Primary antibody, specific to CD9, Mouse IgG1

Validated

ExactAb™

Azide Free

0.5 mg/mL

Application:

Flow Cytometry

Features and benefitsView More⌵

Host species: Mouse
Species reactivity(Reacts with): Human
Isotype: Mouse IgG1
Clone Number: HI9a
Conjugation: Biotin

Item Number

Ab180142

Grouped product items
SKU	Size	Availability	Price	Qty
Ab180142-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!	10μg	10	$39.90	+-
Ab180142-25μg	25μg	5	$59.90	+-
Ab180142-100μg	100μg	1	$159.90	+-

mAb (HI9a); Mouse anti Human CD9 Antibody (Biotin); Flow

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Click to view target information: CD9

Accession#: P21926 (5) CD9 (8) Gene ID: 928 (5) TSPAN29 (4)

Basic Description

Product Name	CD9 Mouse mAb (Biotin) - Primary antibody, specific to CD9, Mouse IgG1
Synonyms	5H9 antigen \| Cell growth-inhibiting gene 2 protein \| Leukocyte antigen MIC3 \| Motility-related protein \| MRP-1 \| p24 \| Tetraspanin-29 \| Tspan-29 \| BA2 \| BA-2/p24 antigen \| BTCC-1 \| CD9 antigen \| CD9 molecule \| CD9 \| Cell growth-inhibiting gene 2 protein
Specifications & Purity	ExactAb™, Validated, Azide Free, 0.5 mg/mL
Host species	Mouse
Specificity	CD9
Immunogen	HL60 cells
Positive Control	Flow: MCF-7
Conjugation	Biotin
Grade	Azide Free, ExactAb™, Validated
Product Description	Application: Flow: ≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Freeze prohibited
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD9 Mouse mAb (Biotin) (Ab180142)

CD9 Mouse mAb (Biotin) (Ab180142) - Flow Cytometry
Flow cytometry analysis of CD9 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD9 Mouse mAb (Biotin) (Ab180142) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume. "> ≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

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C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303581	Certificate of Analysis	Mar 22, 2024	Ab180142
ZJ24F0303580	Certificate of Analysis	Mar 22, 2024	Ab180142
ZJ24F0303579	Certificate of Analysis	Mar 22, 2024	Ab180142
ZJ24R0200266	Certificate of Analysis	Feb 02, 2024	Ab180142

References

1. Miyake, M M, Koyama, M M, Seno, M M and Ikeyama, S S.. (1991) Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility.. The Journal of experimental medicine, (1): [PMID:1720807]
2. Boucheix, C C and 6 more authors.. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.. The Journal of biological chemistry, (5): [PMID:1840589]
3. Lanza, F F and 8 more authors.. (1991) cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins.. The Journal of biological chemistry, (5): [PMID:2037603]
4. Higashihara, M M, Takahata, K K, Yatomi, Y Y, Nakahara, K K and Kurokawa, K K.. (1990) Purification and partial characterization of CD9 antigen of human platelets.. FEBS letters, (21): [PMID:2358073]
5. Masellis-Smith, A A and Shaw, A R AR.. (1994) CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:7511626]
6. Ikeyama, S S, Koyama, M M, Yamaoko, M M, Sasada, R R and Miyake, M M.. (1993) Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.. The Journal of experimental medicine, (1): [PMID:8478605]
7. Rubinstein, E E and 6 more authors.. (1993) Organization of the human CD9 gene.. Genomics, [PMID:8486348]
8. Charrin, S S and 7 more authors.. (2001) The major CD9 and CD81 molecular partner. Identification and characterization of the complexes.. The Journal of biological chemistry, (27): [PMID:11278880]
9. Stipp, C S CS, Kolesnikova, T V TV and Hemler, M E ME.. (2001) EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily.. The Journal of biological chemistry, (2): [PMID:11504738]
10. Charrin, Stéphanie S and 5 more authors.. (2002) Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation.. FEBS letters, (10): [PMID:11959120]
11. Takeda, Yoshito Y and 16 more authors.. (2003) Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes.. The Journal of cell biology, (9): [PMID:12796480]
12. Kovalenko, Oleg V OV, Yang, Xiuwei X, Kolesnikova, Tatiana V TV and Hemler, Martin E ME.. (2004) Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking.. The Biochemical journal, (15): [PMID:14556650]
13. Higginbottom, Adrian A and 6 more authors.. (2003) Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion.. Biochemical and biophysical research communications, (7): [PMID:14575715]
14. Kobayashi, H H and 7 more authors.. (2004) The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation.. Clinical and experimental immunology, [PMID:15196249]
15. Sharma, Chandan C, Yang, Xiuwei H XH and Hemler, Martin E ME.. (2008) DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151.. Molecular biology of the cell, [PMID:18508921]
16. Nakazawa, Youya Y and 7 more authors.. (2008) Tetraspanin family member CD9 inhibits Aggrus/podoplanin-induced platelet aggregation and suppresses pulmonary metastasis.. Blood, (1): [PMID:18541721]
17. Rocha-Perugini, V V and 7 more authors.. (2013) CD81 controls sustained T cell activation signaling and defines the maturation stages of cognate immunological synapses.. Molecular and cellular biology, [PMID:23858057]
18. Hulme, Rachel S RS, Higginbottom, Adrian A, Palmer, John J, Partridge, Lynda J LJ and Monk, Peter N PN.. (2014) Distinct regions of the large extracellular domain of tetraspanin CD9 are involved in the control of human multinucleated giant cell formation.. PloS one, [PMID:25551757]
19. Yu, Jessica J and 5 more authors.. (2017) The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3.. The Biochemical journal, (15): [PMID:27993971]

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CD9 Mouse mAb (Biotin) - Primary antibody, specific to CD9, Mouse IgG1

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Basic Description

Product Properties

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Associated Targets(Human)

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