mAb (HI9a); Mouse anti Human CD9 Antibody; Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab095487-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $26.90 | |
| Ab095487-50μg | 50μg | In stock | $69.90 | |
| Ab095487-100μg | 100μg | In stock | $109.90 | |
| Ab095487-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,029.90 |
mAb (HI9a); Mouse anti Human CD9 Antibody; Flow; Unconjugated
| Product Name | CD9 Mouse mAb - Primary antibody, specific to CD9, Mouse IgG1 |
|---|---|
| Synonyms | BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSP |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD9 |
| Immunogen | HL60 cells |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD9 Antibody, Monoclonal (HI9a), could be used for Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot. |
CD9 Mouse mAb (Ab095487) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling CD9 (red) with CD9 Mouse mAb (Ab095487) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | < 0.5µg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101618 | Certificate of Analysis | Nov 08, 2023 | Ab095487 |
| ZJ23F1101619 | Certificate of Analysis | Nov 08, 2023 | Ab095487 |
| 1. Miyake, M M, Koyama, M M, Seno, M M and Ikeyama, S S.. (1991) Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility.. The Journal of experimental medicine, (1): [PMID:1720807] |
| 2. Boucheix, C C and 6 more authors.. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.. The Journal of biological chemistry, (5): [PMID:1840589] |
| 3. Lanza, F F and 8 more authors.. (1991) cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins.. The Journal of biological chemistry, (5): [PMID:2037603] |
| 4. Higashihara, M M, Takahata, K K, Yatomi, Y Y, Nakahara, K K and Kurokawa, K K.. (1990) Purification and partial characterization of CD9 antigen of human platelets.. FEBS letters, (21): [PMID:2358073] |
| 5. Masellis-Smith, A A and Shaw, A R AR.. (1994) CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:7511626] |
| 6. Ikeyama, S S, Koyama, M M, Yamaoko, M M, Sasada, R R and Miyake, M M.. (1993) Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.. The Journal of experimental medicine, (1): [PMID:8478605] |
| 7. Rubinstein, E E and 6 more authors.. (1993) Organization of the human CD9 gene.. Genomics, [PMID:8486348] |
| 8. Charrin, S S and 7 more authors.. (2001) The major CD9 and CD81 molecular partner. Identification and characterization of the complexes.. The Journal of biological chemistry, (27): [PMID:11278880] |
| 9. Stipp, C S CS, Kolesnikova, T V TV and Hemler, M E ME.. (2001) EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily.. The Journal of biological chemistry, (2): [PMID:11504738] |
| 10. Charrin, Stéphanie S and 5 more authors.. (2002) Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation.. FEBS letters, (10): [PMID:11959120] |
| 11. Takeda, Yoshito Y and 16 more authors.. (2003) Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes.. The Journal of cell biology, (9): [PMID:12796480] |
| 12. Kovalenko, Oleg V OV, Yang, Xiuwei X, Kolesnikova, Tatiana V TV and Hemler, Martin E ME.. (2004) Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking.. The Biochemical journal, (15): [PMID:14556650] |
| 13. Higginbottom, Adrian A and 6 more authors.. (2003) Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion.. Biochemical and biophysical research communications, (7): [PMID:14575715] |
| 14. Kobayashi, H H and 7 more authors.. (2004) The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation.. Clinical and experimental immunology, [PMID:15196249] |
| 15. Sharma, Chandan C, Yang, Xiuwei H XH and Hemler, Martin E ME.. (2008) DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151.. Molecular biology of the cell, [PMID:18508921] |
| 16. Nakazawa, Youya Y and 7 more authors.. (2008) Tetraspanin family member CD9 inhibits Aggrus/podoplanin-induced platelet aggregation and suppresses pulmonary metastasis.. Blood, (1): [PMID:18541721] |
| 17. Rocha-Perugini, V V and 7 more authors.. (2013) CD81 controls sustained T cell activation signaling and defines the maturation stages of cognate immunological synapses.. Molecular and cellular biology, [PMID:23858057] |
| 18. Hulme, Rachel S RS, Higginbottom, Adrian A, Palmer, John J, Partridge, Lynda J LJ and Monk, Peter N PN.. (2014) Distinct regions of the large extracellular domain of tetraspanin CD9 are involved in the control of human multinucleated giant cell formation.. PloS one, [PMID:25551757] |
| 19. Yu, Jessica J and 5 more authors.. (2017) The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3.. The Biochemical journal, (15): [PMID:27993971] |
| 20. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
