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CD9 Mouse mAb - Primary antibody, specific to CD9, Mouse IgG1

  • Carrier Free
  • ExactAb™
  • Validated
  • Lot by Lot
Features and benefits
  • Short Overview:

    mAb (HI9a); Mouse anti Human CD9 Antibody; Flow; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
    Application:
  • Flow Cytometry
Item Number
Ab095487
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab095487-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$26.90
Ab095487-50μg
50μg
In stock
$69.90
Ab095487-100μg
100μg
In stock
$109.90
Ab095487-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,029.90

mAb (HI9a); Mouse anti Human CD9 Antibody; Flow; Unconjugated

View related series
Accession#:P21926 CD9 Gene ID:928

Basic Description

Product NameCD9 Mouse mAb - Primary antibody, specific to CD9, Mouse IgG1
SynonymsBA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSP
Specifications & PurityExactAb™, Validated, Carrier Free, Lot by Lot
Host speciesMouse
SpecificityCD9
ImmunogenHL60 cells
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD9 Antibody, Monoclonal (HI9a), could be used for Flow and so on.
Application:
Flow < 0.5µg/100w cells
Protein Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageShipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD9 Mouse mAb (Ab095487) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling CD9 (red) with CD9 Mouse mAb (Ab095487) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets

CD9 Tbio CD9 antigen 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101618Certificate of AnalysisNov 08, 2023 Ab095487
ZJ23F1101619Certificate of AnalysisNov 08, 2023 Ab095487

Related Documents

References

1. Miyake, M M, Koyama, M M, Seno, M M and Ikeyama, S S..  (1991)  Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility..  The Journal of experimental medicine,    (1):   [PMID:1720807]
2. Boucheix, C C and 6 more authors..  (1991)  Molecular cloning of the CD9 antigen. A new family of cell surface proteins..  The Journal of biological chemistry,    (5):   [PMID:1840589]
3. Lanza, F F and 8 more authors..  (1991)  cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins..  The Journal of biological chemistry,    (5):   [PMID:2037603]
4. Higashihara, M M, Takahata, K K, Yatomi, Y Y, Nakahara, K K and Kurokawa, K K..  (1990)  Purification and partial characterization of CD9 antigen of human platelets..  FEBS letters,    (21):   [PMID:2358073]
5. Masellis-Smith, A A and Shaw, A R AR..  (1994)  CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:7511626]
6. Ikeyama, S S, Koyama, M M, Yamaoko, M M, Sasada, R R and Miyake, M M..  (1993)  Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA..  The Journal of experimental medicine,    (1):   [PMID:8478605]
7. Rubinstein, E E and 6 more authors..  (1993)  Organization of the human CD9 gene..  Genomics,      [PMID:8486348]
8. Charrin, S S and 7 more authors..  (2001)  The major CD9 and CD81 molecular partner. Identification and characterization of the complexes..  The Journal of biological chemistry,    (27):   [PMID:11278880]
9. Stipp, C S CS, Kolesnikova, T V TV and Hemler, M E ME..  (2001)  EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily..  The Journal of biological chemistry,    (2):   [PMID:11504738]
10. Charrin, Stéphanie S and 5 more authors..  (2002)  Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation..  FEBS letters,    (10):   [PMID:11959120]
11. Takeda, Yoshito Y and 16 more authors..  (2003)  Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes..  The Journal of cell biology,    (9):   [PMID:12796480]
12. Kovalenko, Oleg V OV, Yang, Xiuwei X, Kolesnikova, Tatiana V TV and Hemler, Martin E ME..  (2004)  Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking..  The Biochemical journal,    (15):   [PMID:14556650]
13. Higginbottom, Adrian A and 6 more authors..  (2003)  Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion..  Biochemical and biophysical research communications,    (7):   [PMID:14575715]
14. Kobayashi, H H and 7 more authors..  (2004)  The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation..  Clinical and experimental immunology,      [PMID:15196249]
15. Sharma, Chandan C, Yang, Xiuwei H XH and Hemler, Martin E ME..  (2008)  DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151..  Molecular biology of the cell,      [PMID:18508921]
16. Nakazawa, Youya Y and 7 more authors..  (2008)  Tetraspanin family member CD9 inhibits Aggrus/podoplanin-induced platelet aggregation and suppresses pulmonary metastasis..  Blood,    (1):   [PMID:18541721]
17. Rocha-Perugini, V V and 7 more authors..  (2013)  CD81 controls sustained T cell activation signaling and defines the maturation stages of cognate immunological synapses..  Molecular and cellular biology,      [PMID:23858057]
18. Hulme, Rachel S RS, Higginbottom, Adrian A, Palmer, John J, Partridge, Lynda J LJ and Monk, Peter N PN..  (2014)  Distinct regions of the large extracellular domain of tetraspanin CD9 are involved in the control of human multinucleated giant cell formation..  PloS one,      [PMID:25551757]
19. Yu, Jessica J and 5 more authors..  (2017)  The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3..  The Biochemical journal,    (15):   [PMID:27993971]

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