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CD9 Mouse mAb (PE) - Primary antibody, specific to CD9, Mouse IgG1

5μL/test

Application:

Flow Cytometry

Features and benefits

Host species: Mouse
Species reactivity(Reacts with): Human
Isotype: Mouse IgG1
Clone Number: HI9a
Conjugation: PE

Item Number

Ab180144

Grouped product items
SKU	Size	Availability	Price
Ab180144-10T (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!	10T	In stock	$79.90
Ab180144-25T	25T	In stock	$119.90
Ab180144-100T	100T	In stock	$259.90

mAb (HI9a); Mouse anti Human CD9 Antibody (PE); Flow

View related series

Click to view target information: CD9

Accession#: P21926 CD9 Gene ID: 928 PE TSPAN29

Basic Description

Product Name	CD9 Mouse mAb (PE) - Primary antibody, specific to CD9, Mouse IgG1
Synonyms	5H9 antigen \| Cell growth-inhibiting gene 2 protein \| Leukocyte antigen MIC3 \| Motility-related protein \| MRP-1 \| p24 \| Tetraspanin-29 \| Tspan-29 \| BA2 \| BA-2/p24 antigen \| BTCC-1 \| CD9 antigen \| CD9 molecule \| CD9 \| Cell growth-inhibiting gene 2 protein
Specifications & Purity	ExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 5μL/test
Host species	Mouse
Specificity	CD9
Immunogen	HL60 cells
Positive Control	Flow: MCF-7
Conjugation	PE
Grade	Azide Free, Em:575nm, Ex:565nm, ExactAb™, Validated
Product Description	Application: Flow: 5μL/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
Purification Method	Protein A purified
Form	Liquid
Concentration	5μL/test
Storage Temp	Store at 2-8°C,Protected from light,Freeze prohibited
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD9 Mouse mAb (PE) (Ab180144) - Flow Cytometry
Flow cytometry analysis of CD9 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD9 Mouse mAb (PE) (Ab180144) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. "> 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303850	Certificate of Analysis	Mar 28, 2024	Ab180144
ZJ24F0303849	Certificate of Analysis	Mar 28, 2024	Ab180144
ZJ24F0303848	Certificate of Analysis	Mar 28, 2024	Ab180144
ZJ24R0200268	Certificate of Analysis	Feb 02, 2024	Ab180144

References

1. Miyake, M M, Koyama, M M, Seno, M M and Ikeyama, S S.. (1991) Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility.. The Journal of experimental medicine, (1): [PMID:1720807]
2. Boucheix, C C and 6 more authors.. (1991) Molecular cloning of the CD9 antigen. A new family of cell surface proteins.. The Journal of biological chemistry, (5): [PMID:1840589]
3. Lanza, F F and 8 more authors.. (1991) cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins.. The Journal of biological chemistry, (5): [PMID:2037603]
4. Higashihara, M M, Takahata, K K, Yatomi, Y Y, Nakahara, K K and Kurokawa, K K.. (1990) Purification and partial characterization of CD9 antigen of human platelets.. FEBS letters, (21): [PMID:2358073]
5. Masellis-Smith, A A and Shaw, A R AR.. (1994) CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:7511626]
6. Ikeyama, S S, Koyama, M M, Yamaoko, M M, Sasada, R R and Miyake, M M.. (1993) Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA.. The Journal of experimental medicine, (1): [PMID:8478605]
7. Rubinstein, E E and 6 more authors.. (1993) Organization of the human CD9 gene.. Genomics, [PMID:8486348]
8. Charrin, S S and 7 more authors.. (2001) The major CD9 and CD81 molecular partner. Identification and characterization of the complexes.. The Journal of biological chemistry, (27): [PMID:11278880]
9. Stipp, C S CS, Kolesnikova, T V TV and Hemler, M E ME.. (2001) EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily.. The Journal of biological chemistry, (2): [PMID:11504738]
10. Charrin, Stéphanie S and 5 more authors.. (2002) Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation.. FEBS letters, (10): [PMID:11959120]
11. Takeda, Yoshito Y and 16 more authors.. (2003) Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes.. The Journal of cell biology, (9): [PMID:12796480]
12. Kovalenko, Oleg V OV, Yang, Xiuwei X, Kolesnikova, Tatiana V TV and Hemler, Martin E ME.. (2004) Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking.. The Biochemical journal, (15): [PMID:14556650]
13. Higginbottom, Adrian A and 6 more authors.. (2003) Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion.. Biochemical and biophysical research communications, (7): [PMID:14575715]
14. Kobayashi, H H and 7 more authors.. (2004) The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation.. Clinical and experimental immunology, [PMID:15196249]
15. Sharma, Chandan C, Yang, Xiuwei H XH and Hemler, Martin E ME.. (2008) DHHC2 affects palmitoylation, stability, and functions of tetraspanins CD9 and CD151.. Molecular biology of the cell, [PMID:18508921]
16. Nakazawa, Youya Y and 7 more authors.. (2008) Tetraspanin family member CD9 inhibits Aggrus/podoplanin-induced platelet aggregation and suppresses pulmonary metastasis.. Blood, (1): [PMID:18541721]
17. Rocha-Perugini, V V and 7 more authors.. (2013) CD81 controls sustained T cell activation signaling and defines the maturation stages of cognate immunological synapses.. Molecular and cellular biology, [PMID:23858057]
18. Hulme, Rachel S RS, Higginbottom, Adrian A, Palmer, John J, Partridge, Lynda J LJ and Monk, Peter N PN.. (2014) Distinct regions of the large extracellular domain of tetraspanin CD9 are involved in the control of human multinucleated giant cell formation.. PloS one, [PMID:25551757]
19. Yu, Jessica J and 5 more authors.. (2017) The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3.. The Biochemical journal, (15): [PMID:27993971]

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