mAb(1B10E12); Mouse anti Human CD99 Antibody; WB, IHC, FCM, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab006798-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| Ab006798-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $237.90 | |
| Ab006798-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $327.90 | |
| Ab006798-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,997.90 |
mAb(1B10E12); Mouse anti Human CD99 Antibody; WB, IHC, FCM, ELISA; Unconjugated
| Product Name | CD99 Mouse mAb - Primary antibody, specific to CD99, Mouse IgG1 |
|---|---|
| Synonyms | Anti-Human CD99 Monoclonal Antibody; CD99 mouse mAb;12E7 antibody; Antigen identified by monoclonal 12E7, Y homolog antibody; Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody; CD99 antibody; CD99 antigen antibody; CD99 molecule antib |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Host species | Mouse |
| Specificity | CD99 |
| Immunogen | Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli. |
| Positive Control | WB: HEK293-6E, THP-1, U937, Molt-4 and L1210 cell lysates. IHC: Bladder cancer tissues, ewing sarcoma tissues FCM: Jurkat cell. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Application: |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | This product will ship in a box containing blue ice at a temperature of 4°C. Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze / thaw cycles. |
CD99 Mouse mAb (Ab006798) - Western Blot
All lanes: CD99 Mouse mAb (Ab006798) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 28&32 kDa
CD99 Mouse mAb (Ab006798) - Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD99 Mouse mAb (Ab006798) (green) and negative control (red).
CD99 Mouse mAb (Ab006798) - IHC
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD99 mouse mAb (Ab006798) with DAB staining.
CD99 Mouse mAb (Ab006798) - IHC
Immunohistochemical analysis of paraffin-embedded human ewing sarcoma tissues using CD99 (Ab006798) mouse mAb with DAB staining.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800756 | Certificate of Analysis | Aug 03, 2023 | Ab006798 |
| ZJ23F0800757 | Certificate of Analysis | Aug 03, 2023 | Ab006798 |
| 1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] |
| 2. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
| 3. Goodfellow, P J PJ and 5 more authors.. (1988) Absence of methylation of a CpG-rich region at the 5' end of the MIC2 gene on the active X, the inactive X, and the Y chromosome.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2456574] |
| 4. Gelin, C C and 6 more authors.. (1989) The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product.. The EMBO journal, [PMID:2479542] |
| 5. Darling, S M SM and 5 more authors.. (1986) Molecular genetics of MIC2: a gene shared by the human X and Y chromosomes.. Cold Spring Harbor symposia on quantitative biology, [PMID:3472717] |
| 6. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 7. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 8. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
