| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab095879-10μl | 10μl | In stock | $76.90 | |
| Ab095879-50μl | 50μl | In stock | $219.90 | |
| Ab095879-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab095879-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
pAb; Rabbit anti Human CDT1/DUP Antibody; WB, ICC, IF; Unconjugated
| Product Name | CDT1/DUP Antibody - Primary antibody, specific to CDT1, Rabbit IgG |
|---|---|
| Synonyms | CDT 1 antibody | cdt1 antibody | CDT1_HUMAN antibody | Chromatin licensing and DNA replication factor 1 antibody | DNA replication factor antibody | DNA replication factor Cdt1 antibody | Double parked antibody | Double parked Drosophila homolog of antibo |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | CDT1 |
| Immunogen | Recombinant protein of human CDT1 (AA 247-546) |
| Positive Control | WB: K562, HEK293, Raji, HeLa and C6 cell lysates. ICC/IF: 293T cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Rabbit anti Human CDT1/DUP Antibody, Polyclonal, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
CDT1/DUP Antibody (Ab095879) - Western Blot
All lanes: CDT1/DUP Antibody (Ab095879) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 60 kDa
Observed band size: 68 kDa
Exposure time: 50.0 s
CDT1/DUP Antibody (Ab095879) - Western Blot
All lanes: CDT1/DUP Antibody (Ab095879) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 68 kDa
Exposure time: 52.9 s
CDT1/DUP Antibody (Ab095879) - IF/ICC
Immunocytochemistry analysis of CDT1 (green) in 293T using CDT1/DUP Antibody (Ab095879), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303164 | Certificate of Analysis | Mar 13, 2024 | Ab095879 |
| ZJ24F0303163 | Certificate of Analysis | Mar 13, 2024 | Ab095879 |
| 1. Nishitani, H H, Lygerou, Z Z, Nishimoto, T T and Nurse, P P.. (2000) The Cdt1 protein is required to license DNA for replication in fission yeast.. Nature, (6): [PMID:10766248] |
| 2. Wohlschlegel, J A JA and 5 more authors.. (2000) Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.. Science (New York, N.Y.), (22): [PMID:11125146] |
| 3. Cook, Jeanette Gowen JG, Chasse, Dawn A D DA and Nevins, Joseph R JR.. (2004) The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells.. The Journal of biological chemistry, (5): [PMID:14672932] |
| 4. Sugimoto, Nozomi N and 6 more authors.. (2004) Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding.. The Journal of biological chemistry, (7): [PMID:14993212] |
| 5. Ballabeni, Andrea A and 5 more authors.. (2004) Human geminin promotes pre-RC formation and DNA replication by stabilizing CDT1 in mitosis.. The EMBO journal, (4): [PMID:15257290] |
| 6. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553] |
| 7. Higa, Leigh Ann LA and 5 more authors.. (2006) L2DTL/CDT2 interacts with the CUL4/DDB1 complex and PCNA and regulates CDT1 proteolysis in response to DNA damage.. Cell cycle (Georgetown, Tex.), [PMID:16861906] |
| 8. Jin, Jianping J, Arias, Emily E EE, Chen, Jing J, Harper, J Wade JW and Walter, Johannes C JC.. (2006) A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1.. Molecular cell, (1): [PMID:16949367] |
| 9. Sansam, Christopher L CL and 7 more authors.. (2006) DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint.. Genes & development, (15): [PMID:17085480] |
| 10. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 11. Miotto, Benoit B and Struhl, Kevin K.. (2008) HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1.. Genes & development, (1): [PMID:18832067] |
| 12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 13. Guernsey, Duane L DL and 22 more authors.. (2011) Mutations in origin recognition complex gene ORC4 cause Meier-Gorlin syndrome.. Nature genetics, (27): [PMID:21358631] |
| 14. Bicknell, Louise S LS and 26 more authors.. (2011) Mutations in the pre-replication complex cause Meier-Gorlin syndrome.. Nature genetics, (27): [PMID:21358632] |
| 15. Pefani, Dafni-Eleutheria DE and 9 more authors.. (2011) Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression.. The Journal of biological chemistry, (1): [PMID:21543332] |
| 16. Miotto, Benoit B and Struhl, Kevin K.. (2011) JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress.. Molecular cell, (7): [PMID:21856198] |
| 17. Varma, Dileep D and 9 more authors.. (2012) Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment.. Nature cell biology, (13): [PMID:22581055] |
| 18. Shen, Zhen Z and 6 more authors.. (2012) Dynamic association of ORCA with prereplicative complex components regulates DNA replication initiation.. Molecular and cellular biology, [PMID:22645314] |
| 19. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 20. Sugimoto, Nozomi N and 11 more authors.. (2015) Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.. Nucleic acids research, (13): [PMID:25990725] |
| 21. Franz, André A and 7 more authors.. (2016) Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression.. Nature communications, (4): [PMID:26842564] |
| 22. Hernández-Pérez, Santiago S and 7 more authors.. (2016) USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication.. Molecular oncology, [PMID:27296872] |
