| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab095961-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $86.90 | |
| Ab095961-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $267.90 | |
| Ab095961-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $387.90 | |
| Ab095961-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,997.90 |
mAb(4E10); Mouse anti Human CEBPA Antibody; WB, Flow, ELISA; Unconjugated
| Product Name | CEBPA Mouse mAb - Primary antibody, specific to CEBPA, IgG1 |
|---|---|
| Synonyms | C/EBP alpha |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Host species | Mouse |
| Specificity | CEBPA |
| Immunogen | Purified recombinant fragment of human CEBPA expressed in E. Coli. |
| Positive Control | WB: THP-1 cell lysate, Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse Monoclonal (4E10) to CEBPA , could be used for WB, Flow and ELISA. |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze/thaw cycle. |
CEBPA Mouse mAb (Ab095961) - Western Blot
All lanes: CEBPA Mouse mAb (Ab095961) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42&26 kDa
Observed band size: 42&26 kDa
CEBPA Mouse mAb (Ab095961) - Flow Cytometry
Flow cytometric analysis of HeLa cells using CEBPA Mouse mAb (Ab095961) at 1/200 dilution (green) and negative control (red).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
">
1/10000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700733 | Certificate of Analysis | Aug 01, 2023 | Ab095961 |
| ZJ23F0700732 | Certificate of Analysis | Aug 01, 2023 | Ab095961 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Antonson, P P and Xanthopoulos, K G KG.. (1995) Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha).. Biochemical and biophysical research communications, (4): [PMID:7575576] |
| 3. Choi, B H BH, Park, G T GT and Rho, H M HM.. (1999) Interaction of hepatitis B viral X protein and CCAAT/ enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter.. The Journal of biological chemistry, (29): [PMID:9915821] |
| 4. Aho, S S and 7 more authors.. (2000) Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors.. The Journal of cell biology, (20): [PMID:10725330] |
| 5. Pabst, T T and 8 more authors.. (2001) Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute myeloid leukemia.. Nature genetics, [PMID:11242107] |
| 6. Snaddon, Jennifer J and 9 more authors.. (2003) Mutations of CEBPA in acute myeloid leukemia FAB types M1 and M2.. Genes, chromosomes & cancer, [PMID:12661007] |
| 7. Müller, Christine C, Calkhoven, Cornelis F CF, Sha, Xiaojing X and Leutz, Achim A.. (2004) The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest.. The Journal of biological chemistry, (20): [PMID:14660596] |
| 8. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 9. Wu, Frederick Y FY and 5 more authors.. (2004) CCAAT/enhancer binding protein alpha binds to the Epstein-Barr virus (EBV) ZTA protein through oligomeric interactions and contributes to cooperative transcriptional activation of the ZTA promoter through direct binding to the ZII and ZIIIB motifs during induction of the EBV lytic cycle.. Journal of virology, [PMID:15078966] |
| 10. Wang, Guo-Li GL, Iakova, Polina P, Wilde, Margie M, Awad, Samir S and Timchenko, Nikolai A NA.. (2004) Liver tumors escape negative control of proliferation via PI3K/Akt-mediated block of C/EBP alpha growth inhibitory activity.. Genes & development, (15): [PMID:15107404] |
| 11. Smith, Matthew L ML, Cavenagh, Jamie D JD, Lister, T Andrew TA and Fitzgibbon, Jude J.. (2004) Mutation of CEBPA in familial acute myeloid leukemia.. The New England journal of medicine, (2): [PMID:15575056] |
| 12. Müller, Christine C, Bremer, Anna A, Schreiber, Sandra S, Eichwald, Sabrina S and Calkhoven, Cornelis F CF.. (2010) Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size.. The EMBO journal, (3): [PMID:20075868] |
| 13. Zaragoza, Katrin K, Bégay, Valérie V, Schuetz, Anja A, Heinemann, Udo U and Leutz, Achim A.. (2010) Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes.. Molecular and cellular biology, [PMID:20176812] |
| 14. Dedhia, Priya H PH and 9 more authors.. (2010) Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia.. Blood, (26): [PMID:20410507] |
| 15. Uljon, Sacha and 7 more authors.. (2016) Structural Basis for Substrate Selectivity of the E3 Ligase COP1.. Structure (London, England : 1993), (3): [PMID:27041596] |
| 16. Brunmeir, Reinhard R and 7 more authors.. (2016) Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.. PLoS genetics, [PMID:27923061] |
