Collagenase from Clostridium histolyticum(Type 2) - ≥125 units/mg dry weight, high purity , CAS No.9001-12-1

Item Number
C128703
Grouped product items
SKUSizeAvailabilityPrice Qty
C128703-250mg
250mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$309.90
C128703-1g
1g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$1,019.90
C128703-5g
5g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$5,114.90

Basic Description

SynonymsCollagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
Specifications & PurityEnzymoPure™, ≥125 units/mg dry weight
Storage TempStore at 2-8°C
Shipped InWet ice
GradeEnzymoPure™
Product Description

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Associated Targets(Human)

HT-29 (80576 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
MCF7 (126967 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SK-MEL-5 (47095 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
A549 (127892 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

Isomeric SMILES CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C
WGK Germany 1
PubChem CID 44384985

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
E2323812Certificate of AnalysisApr 07, 2023 C128703
E2323816Certificate of AnalysisApr 07, 2023 C128703
E2323819Certificate of AnalysisApr 07, 2023 C128703
H2219162Certificate of AnalysisAug 24, 2022 C128703

Safety and Hazards(GHS)

Pictogram(s) GHS08
Signal Warning
Hazard Statements

H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P501:Dispose of contents/container to ...

P284:[In case of inadequate ventilation] Wear respiratory protection.

WGK Germany 1

Related Documents

Citations of This Product

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke.  (2023)  Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma.  BIOMEDICINE & PHARMACOTHERAPY,  165  (115096).  [PMID:37421781] [10.1016/j.biopha.2023.115096]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang.  (2023)  The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  242  (124820).  [PMID:37178890] [10.1016/j.ijbiomac.2023.124820]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu.  (2023)  A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  668  (131410).  [PMID:] [10.1016/j.colsurfa.2023.131410]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang.  (2023)  Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,  631  (122489).  [PMID:36521639] [10.1016/j.ijpharm.2022.122489]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen.  (2023)  Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding.  Materials Today Bio,  18  (100506).  [PMID:36471892] [10.1016/j.mtbio.2022.100506]
6. Congying Wei, Weili Shi, Chuangqi Zhao, Shuai Yang, Jiajia Zheng, JinPan Zhong, Tianyi Zhao, Simin Kong, Xi Gong, Mingjie Liu.  (2022)  Superwetting Injectable Hydrogel with Ultrastrong and Fast Tissue Adhesion for Minimally Invasive Hemostasis.  Advanced Healthcare Materials,  12  (3): (2201799).  [PMID:36333905] [10.1002/adhm.202201799]
7. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng.  (2022)  In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration.  Smart Materials and Structures,  31  (10): (105002).  [PMID:] [10.1088/1361-665X/ac884a]
8. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu.  (2020)  Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,  31  (17): (2199-2219).  [PMID:32663418] [10.1080/09205063.2020.1796229]
9. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li.  (2020)  Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair.  Materials Science & Engineering C-Materials for Biological Applications,  109  (110538).  [PMID:32228945] [10.1016/j.msec.2019.110538]
10. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu.  (2019)  Highly elastomeric photocurable silk hydrogels.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  134  (838).  [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068]
11. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen.  (2019)  In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing.  Materials Science & Engineering C-Materials for Biological Applications,  101  (487).  [PMID:31029343] [10.1016/j.msec.2019.03.093]
12. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen.  (2018)  Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation.  Materials Science & Engineering C-Materials for Biological Applications,  92  (447).  [PMID:30184770] [10.1016/j.msec.2018.06.072]

References

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke.  (2023)  Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma.  BIOMEDICINE & PHARMACOTHERAPY,  165  (115096).  [PMID:37421781] [10.1016/j.biopha.2023.115096]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang.  (2023)  The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  242  (124820).  [PMID:37178890] [10.1016/j.ijbiomac.2023.124820]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu.  (2023)  A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  668  (131410).  [PMID:] [10.1016/j.colsurfa.2023.131410]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang.  (2023)  Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,  631  (122489).  [PMID:36521639] [10.1016/j.ijpharm.2022.122489]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen.  (2023)  Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding.  Materials Today Bio,  18  (100506).  [PMID:36471892] [10.1016/j.mtbio.2022.100506]
6. Congying Wei, Weili Shi, Chuangqi Zhao, Shuai Yang, Jiajia Zheng, JinPan Zhong, Tianyi Zhao, Simin Kong, Xi Gong, Mingjie Liu.  (2022)  Superwetting Injectable Hydrogel with Ultrastrong and Fast Tissue Adhesion for Minimally Invasive Hemostasis.  Advanced Healthcare Materials,  12  (3): (2201799).  [PMID:36333905] [10.1002/adhm.202201799]
7. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng.  (2022)  In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration.  Smart Materials and Structures,  31  (10): (105002).  [PMID:] [10.1088/1361-665X/ac884a]
8. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu.  (2020)  Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,  31  (17): (2199-2219).  [PMID:32663418] [10.1080/09205063.2020.1796229]
9. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li.  (2020)  Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair.  Materials Science & Engineering C-Materials for Biological Applications,  109  (110538).  [PMID:32228945] [10.1016/j.msec.2019.110538]
10. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu.  (2019)  Highly elastomeric photocurable silk hydrogels.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  134  (838).  [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068]
11. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen.  (2019)  In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing.  Materials Science & Engineering C-Materials for Biological Applications,  101  (487).  [PMID:31029343] [10.1016/j.msec.2019.03.093]
12. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen.  (2018)  Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation.  Materials Science & Engineering C-Materials for Biological Applications,  92  (447).  [PMID:30184770] [10.1016/j.msec.2018.06.072]

Solution Calculators