Collagenase NB 1 From Clostridium Histolyticum - Premium Grade,≥ 3.00 U/mg, high purity , CAS No.9001-12-1

Item Number

C278986

• Synonyms: Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
• Specifications & Purity: EnzymoPure™, Premium pure, ≥ 3.00 U/mg
• Storage Temp: Store at 2-8°C
• Shipped In: Wet ice
• Grade: Premium pure, EnzymoPure™

Product Description

Collagenase NB 1 is chromatographically highly purified; therefore it contains a very high collagenolytic activity. It is largely free from additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as endotoxins.

Specifications

Contains chromatographically highly purified class I and class II collagenase (1).

Largely free from clostripain, trypsin-like protease and neutral protease.

Vial contains not less than 2000 PZ U collagenase.

Activity (Wünsch): ≥ 3.00 U/mg

Endotoxin: ≤ 10.0 EU/mg (Ph. Eur.)

(Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Application

Collagenase NB 1 is, mostly in combination with Neutral Protease NB, suitable for cell isolation from several tissue types.

References and Definitions

Unit definition: 

Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.