Collagenase NB 1 From Clostridium Histolyticum - Premium Grade,≥ 3.00 U/mg, high purity , CAS No.9001-12-1

Item Number
C278986
Grouped product items
SKUSizeAvailabilityPrice Qty
C278986-2000U
2000U
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$13,335.90

Tissue dissociators, dissociated tissue and monolayer cells

Basic Description

Synonyms9001-12-1 | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene | Collagenase I | Collagenase Type II | CHEMBL1
Specifications & PurityEnzymoPure™, Premium pure, ≥ 3.00 U/mg
Storage TempStore at 2-8°C
Shipped InWet ice
GradePremium pure, EnzymoPure™
Product Description

Collagenase NB 1 is chromatographically highly purified; therefore it contains a very high collagenolytic activity. It is largely free from additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as endotoxins.


Specifications

Contains chromatographically highly purified class I and class II collagenase (1).

Largely free from clostripain, trypsin-like protease and neutral protease.

Vial contains not less than 2000 PZ U collagenase.

Activity (Wünsch): ≥ 3.00 U/mg

Endotoxin: ≤ 10.0 EU/mg (Ph. Eur.)

(Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]


Application

Collagenase NB 1 is, mostly in combination with Neutral Protease NB, suitable for cell isolation from several tissue types.


References and Definitions

Unit definition: 

Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).


References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Associated Targets(Human)

HT-29 80576 Activities

Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

MCF7 126967 Activities

Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

SK-MEL-5 47095 Activities

Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

A549 127892 Activities

Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Malme-3M 44254 Activities

Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Associated Targets(non-human)

Names and Identifiers

Enzyme Commission Number3.4.24.3
Isomeric SMILES CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C
WGK Germany 1
PubChem CID 44384985

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Safety and Hazards(GHS)

Pictogram(s) GHS08
Signal Warning
Hazard Statements

H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P501:Dispose of contents/container to ...

P284:[In case of inadequate ventilation] Wear respiratory protection.

WGK Germany 1

Related Documents

Citations of This Product

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke.  (2023)  Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma.  BIOMEDICINE & PHARMACOTHERAPY,  165  (115096).  [PMID:37421781]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang.  (2023)  The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  242  (124820).  [PMID:37178890]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu.  (2023)  A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  668  (131410).  [PMID:]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang.  (2023)  Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,  631  (122489).  [PMID:36521639]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen.  (2023)  Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding.  Materials Today Bio,  18  (100506).  [PMID:36471892]
6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng.  (2022)  In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration.  Smart Materials and Structures,  31  (10): (105002).  [PMID:]

References

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke.  (2023)  Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma.  BIOMEDICINE & PHARMACOTHERAPY,  165  (115096).  [PMID:37421781]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang.  (2023)  The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  242  (124820).  [PMID:37178890]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu.  (2023)  A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  668  (131410).  [PMID:]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang.  (2023)  Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,  631  (122489).  [PMID:36521639]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen.  (2023)  Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding.  Materials Today Bio,  18  (100506).  [PMID:36471892]
6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng.  (2022)  In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration.  Smart Materials and Structures,  31  (10): (105002).  [PMID:]

Solution Calculators