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Collagenase NB 1 From Clostridium Histolyticum - Premium Grade,≥ 3.00 U/mg, high purity , CAS No.9001-12-1

11 Citations
Item Number
C278986
Grouped product items
SKUSizeAvailabilityPrice Qty
C278986-2000U
2000U
Available within 8-12 weeks(?)
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$13,335.90
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Tissue dissociators, dissociated tissue and monolayer cells

Basic Description

SynonymsCollagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
Specifications & PurityEnzymoPure™, Premium pure, ≥ 3.00 U/mg
Storage TempStore at 2-8°C
Shipped InWet ice
GradePremium pure, EnzymoPure™
Product Description

Collagenase NB 1 is chromatographically highly purified; therefore it contains a very high collagenolytic activity. It is largely free from additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as endotoxins.


Specifications

Contains chromatographically highly purified class I and class II collagenase (1).

Largely free from clostripain, trypsin-like protease and neutral protease.

Vial contains not less than 2000 PZ U collagenase.

Activity (Wünsch): ≥ 3.00 U/mg

Endotoxin: ≤ 10.0 EU/mg (Ph. Eur.)

(Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]


Application

Collagenase NB 1 is, mostly in combination with Neutral Protease NB, suitable for cell isolation from several tissue types.


References and Definitions

Unit definition: 

Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).


References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Associated Targets(Human)

HT-29 (80576 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
MCF7 (126967 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SK-MEL-5 (47095 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
A549 (127892 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

Enzyme Commission Number3.4.24.3
Isomeric SMILES CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C
WGK Germany 1
PubChem CID 44384985

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Safety and Hazards(GHS)

Pictogram(s) GHS08
Signal Warning
Hazard Statements

H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P501:Dispose of contents/container to ...

P284:[In case of inadequate ventilation] Wear respiratory protection.

WGK Germany 1

Related Documents

 SDS

 Specification Sheet

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke.  (2023)  Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma.  BIOMEDICINE & PHARMACOTHERAPY,  165  (115096).  [PMID:37421781] [10.1016/j.biopha.2023.115096]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang.  (2023)  The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  242  (124820).  [PMID:37178890] [10.1016/j.ijbiomac.2023.124820]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu.  (2023)  A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  668  (131410).  [PMID:] [10.1016/j.colsurfa.2023.131410]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang.  (2023)  Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,  631  (122489).  [PMID:36521639] [10.1016/j.ijpharm.2022.122489]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen.  (2023)  Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding.  Materials Today Bio,  18  (100506).  [PMID:36471892] [10.1016/j.mtbio.2022.100506]
6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng.  (2022)  In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration.  Smart Materials and Structures,  31  (10): (105002).  [PMID:] [10.1088/1361-665X/ac884a]
7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu.  (2020)  Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,  31  (17): (2199-2219).  [PMID:32663418] [10.1080/09205063.2020.1796229]
8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li.  (2020)  Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair.  Materials Science & Engineering C-Materials for Biological Applications,  109  (110538).  [PMID:32228945] [10.1016/j.msec.2019.110538]
9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu.  (2019)  Highly elastomeric photocurable silk hydrogels.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  134  (838).  [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068]
10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen.  (2019)  In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing.  Materials Science & Engineering C-Materials for Biological Applications,  101  (487).  [PMID:31029343] [10.1016/j.msec.2019.03.093]
11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen.  (2018)  Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation.  Materials Science & Engineering C-Materials for Biological Applications,  92  (447).  [PMID:30184770] [10.1016/j.msec.2018.06.072]

References

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke.  (2023)  Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma.  BIOMEDICINE & PHARMACOTHERAPY,  165  (115096).  [PMID:37421781] [10.1016/j.biopha.2023.115096]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang.  (2023)  The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  242  (124820).  [PMID:37178890] [10.1016/j.ijbiomac.2023.124820]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu.  (2023)  A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,  668  (131410).  [PMID:] [10.1016/j.colsurfa.2023.131410]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang.  (2023)  Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury.  INTERNATIONAL JOURNAL OF PHARMACEUTICS,  631  (122489).  [PMID:36521639] [10.1016/j.ijpharm.2022.122489]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen.  (2023)  Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding.  Materials Today Bio,  18  (100506).  [PMID:36471892] [10.1016/j.mtbio.2022.100506]
6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng.  (2022)  In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration.  Smart Materials and Structures,  31  (10): (105002).  [PMID:] [10.1088/1361-665X/ac884a]
7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu.  (2020)  Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold.  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,  31  (17): (2199-2219).  [PMID:32663418] [10.1080/09205063.2020.1796229]
8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li.  (2020)  Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair.  Materials Science & Engineering C-Materials for Biological Applications,  109  (110538).  [PMID:32228945] [10.1016/j.msec.2019.110538]
9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu.  (2019)  Highly elastomeric photocurable silk hydrogels.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  134  (838).  [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068]
10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen.  (2019)  In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing.  Materials Science & Engineering C-Materials for Biological Applications,  101  (487).  [PMID:31029343] [10.1016/j.msec.2019.03.093]
11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen.  (2018)  Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation.  Materials Science & Engineering C-Materials for Biological Applications,  92  (447).  [PMID:30184770] [10.1016/j.msec.2018.06.072]

Solution Calculators

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