Collagenase NB 6 From Clostridium Histolyticum - ≥ 0.100 U/mg, high purity , CAS No.9001-12-1

Item Number

C278976

• Synonyms: Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
• Specifications & Purity: EnzymoPure™, GMP, ≥ 0.100 U/mg
• Storage Temp: Store at 2-8°C
• Shipped In: Wet ice
• Grade: GMP, EnzymoPure™

Product Description

Collagenase NB 6 is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation.

Specifications

Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.

Activity (Wünsch): ≥ 0.100 U/mg

Microbiological examination: Sterility, absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).

Sterile according to Ph. Eur.

 (Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Applications

Collagenase NB 6  is suitable for cell isolation from various tissue types intended for clinical applications.

References and Definitions

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of

1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51