Collagenase NB 8 From Clostridium Histolyticum - Broad Range,≥ 0.90 U/mg, high purity , CAS No.9001-12-1

Item Number

C278989

• Synonyms: Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
• Specifications & Purity: EnzymoPure™, Broad Range,≥ 0.90 U/mg
• Storage Temp: Store at 2-8°C
• Shipped In: Wet ice
• Grade: EnzymoPure™

Product Description

Collagenase NB 8 is chromatographically purified; therefore it contains a high collagenolytic activity. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced.

Application

Collagenase NB 8 is suitable for dissociation of a broad variety of tissue types.

Specifications

Contains class I and class II collagenase (1) as well as reduced levels of clostripain, trypsin-like activities and neutral protease.

Chromatographically purified.

Activity (Wünsch): ≥ 0.90 U/mg

Endotoxin: ≤ 100 EU/mg (Ph. Eur.)

(Clostridiopeptidase A)

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

References and Definitions

Unit definition: 

Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.