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Connexin 43/GJA1 (phospho S368) Antibody - Primary antibody, specific to GJA1 (phospho S368), Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefitsView More
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab097298
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab097298-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab097298-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab097298-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab097298-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

pAb; Rabbit anti Human Connexin 43/GJA1 (phospho S368) Antibody; WB, IHC; Unconjugated

Basic Description

Product NameConnexin 43/GJA1 (phospho S368) Antibody - Primary antibody, specific to GJA1 (phospho S368), Rabbit IgG
SynonymsConnexin 43(phospho-Ser368) | Connexin 43(phospho Ser368) | Connexin 43(phospho S368) | Connexin 43 | Connexin43v Cx 43v CX43 | DFNB38 | Gap junction 43 kDa heart protein | Connexin-43 | Gap junction alpha 1 protein | Gap junction protein alpha 1 43kDa (c
Specifications & PurityValidated, 1.0 mg/mL
Host speciesRabbit
SpecificityGJA1 (phospho S368)
ImmunogenA synthetic peptide derived from human Connexin 43/GJA1 (phospho S368) (AA 332-381).
Positive ControlWB: K562 treated with 200ng/mL PMA for 10 min lysates. IHC: Human breast carcinoma tissue.
ConjugationUnconjugated
GradeValidated
Product Description

Rabbit polyclonal to Connexin 43/GJA1 (phospho S368), could be used for WB, IHC and so on.
Applications:
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
Protein Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150kDa
Purification MethodImmunogen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - Western Blot
All lanes: Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 31, 37 kDa
The truncated isoforms are detected as distinct bands on the western blot lysate from the left ventricle of non-failing human heart tissue . In addition to 43 kDa full length isoform, the largest 3 isoforms (32 kDa, 29 kDa and 26 kDa in size) contain 2 transmembrane domains, part of intracellular loop, and the C-terminus. The smallest 3 isoforms are 20 kDa, 11 kDa, and 7 kDa in size and include part of the C-terminal tail with the 20 kDa isoform also containing part of the last transmembrane domain (PMID: 28576298).
Exposure time: 26.2 s

Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43/GJA1 (phospho S368) Antibody (Ab097298). The picture on the right is blocked with the phosphor peptide.

Associated Targets(Human)

GJA1 Tbio Gap junction alpha-1 protein (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

IHC1/100-1/300

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1/100-1/300

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
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