COX IV Mouse mAb - Primary antibody, specific to COX4I1, Mouse IgG1

    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Goat,Hamster,Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 4D11-B3-E8
  • Conjugation: Unconjugated
Item Number
Ab097414
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab097414-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab097414-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$147.90
Ab097414-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab097414-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,617.90

mAb (4D11-B3-E8); Mouse anti Human COX IV Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated

Basic Description

Product NameCOX IV Mouse mAb - Primary antibody, specific to COX4I1, Mouse IgG1
SynonymsAL024441 antibody | COX 4 antibody | COX IV 1 antibody | COX IV antibody | COX IV-1 antibody | Cox4 antibody | COX41_HUMAN antibody | Cox4a antibody | COX4B antibody | COX4I1 antibody | COX4I2 antibody | COX4L2 antibody | COXIV antibody | Cytochrome c oxi
Specifications & PurityExactAb™, Validated, 2.0 mg/mL
Host speciesMouse
SpecificityCOX4I1
ImmunogenA synthetic peptide derived from human COX IV (AA 1-169).
Positive ControlWB: Jurkat, NIH/3T3, HeLa, C6, K562, MCF7, THP-1, Raji; IHC: Human colorectal carcinoma; ICC/IF: HeLa cells; IP: HeLa.
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Mouse anti Human COX IV Antibody, Monoclonal antibody(4D11-B3-E8), could be used for WB, IHC, ICC, IF, Flow, IP and so on.

Application:

WB: 1/1000 

IHC: 1/50-1/200

ICC/IF: 1/100-1/200 

Flow: 1/100 

IP: 1/20 

Protein Function

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Product Properties

IsotypeMouse IgG1
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration2.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

COX IV Mouse mAb (Ab097414) - Western Blot
All lanes: COX IV Mouse mAb (Ab097414) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5.0 s

COX IV Mouse mAb (Ab097414) - ICC/IF
Immunocytochemistry analysis of COX IV (green) in HeLa cells using COX IV Mouse mAb (Ab097414).

COX IV Mouse mAb (Ab097414) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using COX IV Mouse mAb (Ab097414). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

COX IV Mouse mAb (Ab097414) - IP
Immunoprecipitation analysis of COX IV (4D11) in HeLa cell lysates using COX IV Mouse mAb (Ab097414)

Associated Targets(Human)

COX4I1 Tbio Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IP

1/20

WB 1/1000

">

1/1000

IHC1/50-1/200

">

1/50-1/200

IF/ICC

1/100-1/200

Flow Cytometry1/100

">

1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800822Certificate of AnalysisAug 16, 2023 Ab097414
ZJ23F0800821Certificate of AnalysisAug 16, 2023 Ab097414

Related Documents

FAQs and Articles

FAQ
Loading Controls for Western Blotting
Flow Cytometry
Flow cytometry analysis
How to choose and use antibodies-primary antibody?
Antibody applications and techniques
Flow cytometry protocol
Immunoprecipitation (IP) lysates and reagents
Flow cytometry cell cycle analysis using propidium iodide DNA staining
Experimental Guide of Sample Preparation for Flow Cytometry
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
General Immunoprecipitation Protocol
RNA Immunoprecipitation (RIP) Protocol
Immobilized IP Protocol

Solution Calculators