A synthetic peptide derived from human COX IV (AA 1-169).
Positive Control
WB: Jurkat, NIH/3T3, HeLa, C6, K562, MCF7, THP-1, Raji; IHC: Human colorectal carcinoma; ICC/IF: HeLa cells; IP: HeLa.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Product Description
Mouse anti Human COX IV Antibody, Monoclonal antibody(4D11-B3-E8), could be used for WB, IHC, ICC, IF, Flow, IP and so on.
Application:
WB: 1/1000
IHC: 1/50-1/200
ICC/IF: 1/100-1/200
Flow: 1/100
IP: 1/20
Protein Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Product Properties
Isotype
Mouse IgG1
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
2.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
COX IV Mouse mAb (Ab097414) - Western Blot All lanes: COX IV Mouse mAb (Ab097414) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 5.0 s
COX IV Mouse mAb (Ab097414) - ICC/IF Immunocytochemistry analysis of COX IV (green) in HeLa cells using COX IV Mouse mAb (Ab097414).
COX IV Mouse mAb (Ab097414) - IHC Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using COX IV Mouse mAb (Ab097414). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
COX IV Mouse mAb (Ab097414) - IP Immunoprecipitation analysis of COX IV (4D11) in HeLa cell lysates using COX IV Mouse mAb (Ab097414)
Associated Targets(Human)
COX4I1TbioCytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)