COX IV Mouse mAb - Primary antibody, specific to COX IV, IgG1

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: AB03/3G1
  • Conjugation: Unconjugated
Item Number
Ab181867
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab181867-10μl (Trial Size)
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10μl
In stock
$99.90
Ab181867-50μl
50μl
In stock
$189.90
Ab181867-100μl
100μl
In stock
$299.90
Ab181867-1ml
1ml
Available within 8-12 weeks(?)
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$1,999.90

mAb (AB03/3G1); Mouse anti Human COX IV Antibody; WB, ELISA, ICC/IF; Unconjugated

Basic Description

Product NameCOX IV Mouse mAb - Primary antibody, specific to COX IV, IgG1
SynonymsAL024441 antibody | COX 4 antibody | COX IV 1 antibody | COX IV antibody | COX IV-1 antibody | Cox4 antibody | COX41_HUMAN antibody | Cox4a antibody | COX4B antibody | COX4I1 antibody | COX4I2 antibody | COX4L2 antibody | COXIV antibody | Cytochrome c oxi
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Host speciesMouse
SpecificityCOX IV
ImmunogenRecombinant full length protein corresponding to Human COX IV
Positive ControlWB: HeLa, HepG2, A431, Jurkat, MCF-7, THP-1, K562, HEK293 and A549 Whole cell Lysates ICC/IF: HeLa cells
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/1,000 - 1/20,000; Predicted molecular weight: 19.6 KDa
ICC/IF: 1/200

Product Properties

IsotypeIgG1
Light Chain Typekappa
SDS-PAGE150.0 KDa
Purification MethodProtein A purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

COX IV Mouse mAb (Ab181867) - Western Blot
All lanes: COX IV Mouse Antibody (Ab181867) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 19.6 kDa
Observed band size: 17.4 kDa
Exposure time: 112.6 s 

COX IV Mouse mAb (Ab181867) - Western Blot
All lanes: COX IV Mouse Antibody (Ab181867) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 19.6 kDa
Observed band size: 17.2 kDa
Exposure time: 13.7 s 

COX IV Mouse mAb (Ab181867) - ICC/IF
IF analysis of COX IV (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with COX IV Mouse mAb (Ab181867) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Associated Targets(Human)

COX4I1 Tbio Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IF/ICC

1/200

WB

1/1,000 - 1/20,000; Predicted molecular weight: 19.6 KDa

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404646Certificate of AnalysisApr 18, 2024 Ab181867
ZJ24F0404647Certificate of AnalysisApr 18, 2024 Ab181867
ZJ24F0404648Certificate of AnalysisApr 18, 2024 Ab181867

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