WB: HeLa, Raji, MCF7, MDA-MB-231 and NIH/3T3 cell lysates. IHC: Human esophagus cancer tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human CSP-C Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.8 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
CSP-C Antibody (Ab097978) - Western Blot All lanes: CSP-C Antibody (Ab097978) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 24 kDa Exposure time: 114.7 s
CSP-C Antibody (Ab097978) - IHC Immunohistochemistry analysis of paraffin-embedded Human esophagus cancer using CSP-C Antibody (Ab097978). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1.Haddad, P P and 5 more authors.. (1991) Structure and evolutionary origin of the human granzyme H gene.. International immunology, [PMID:2049336]
2.Meier, M M and 9 more authors.. (1990) Cloning of a gene that encodes a new member of the human cytotoxic cell protease family.. Biochemistry, (1):[PMID:2193684]
3.Klein, J L JL, Selvakumar, A A, Trapani, J A JA and Dupont, B B.. (1990) Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C).. Tissue antigens, [PMID:2402757]
4.Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6):[PMID:12508121]
5.Wang, Li L and 8 more authors.. (2012) Structural insights into the substrate specificity of human granzyme H: the functional roles of a novel RKR motif.. Journal of immunology (Baltimore, Md. : 1950), (15):[PMID:22156497]
6.Wang, Li L and 13 more authors.. (2013) Identification of SERPINB1 as a physiological inhibitor of human granzyme H.. Journal of immunology (Baltimore, Md. : 1950), (1):[PMID:23269243]
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