| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab099205-10μl | 10μl | In stock | $66.90 | |
| Ab099205-50μl | 50μl | In stock | $199.90 | |
| Ab099205-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab099205-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
mAb(3G4-D11-D7); Mouse anti Human DBC-1 Antibody; WB, IF, ICC; Unconjugated
| Product Name | DBC-1 Mouse mAb - Primary antibody, specific to CCAR2, Mouse IgG2a |
|---|---|
| Synonyms | DBC1 | DBC-1 | NET35 | p30DBC | p30 DBC | KIAA1967 | CCAR2 antibody | Cell cycle and apoptosis regulator 2 antibody | Cell division cycle and apoptosis regulator protein 2 antibody | DBC-1 antibody | DBC.1 antibody | DBC1 antibody | DBIRD complex subunit |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | CCAR2 |
| Immunogen | Recombinant protein of human DBC1 (AA 1-50) |
| Positive Control | WB: HeLa, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. IP: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human DBC-1 Antibody, Monoclonal(3G4-D11-D7), could be used for WB, IF, ICC and so on . |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
DBC-1 Mouse mAb (Ab099205) - Western Blot
All lanes: DBC-1 Mouse mAb (Ab099205) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 126 kDa
Exposure time: 10.0 s
DBC-1 Mouse mAb (Ab099205) - IF/ICC
Immunocytochemistry analysis of DBC-1 in HeLa cells using DBC-1 Mouse mAb (Ab099205).
DBC-1 Mouse mAb (Ab099205) - IP
Immunoprecipitation analysis of DBC-1 in HeLa lysates using DBC-1 Mouse mAb (Ab099205).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IP | 1/20 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303237 | Certificate of Analysis | Mar 14, 2024 | Ab099205 |
| ZJ24F0303236 | Certificate of Analysis | Mar 14, 2024 | Ab099205 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Nagase, T T, Kikuno, R R and Ohara, O O.. (2001) Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins.. DNA research : an international journal for rapid publication of reports on genes and genomes, (31): [PMID:11853319] |
| 4. Hamaguchi, Masaaki M and 9 more authors.. (2002) DBC2, a candidate for a tumor suppressor gene involved in breast cancer.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:12370419] |
| 5. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571] |
| 6. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 7. Kim, Ja-Eun JE, Chen, Junjie J and Lou, Zhenkun Z.. (2008) DBC1 is a negative regulator of SIRT1.. Nature, (31): [PMID:18235501] |
| 8. Zhao, Wenhui W and 5 more authors.. (2008) Negative regulation of the deacetylase SIRT1 by DBC1.. Nature, (31): [PMID:18235502] |
| 9. Tegha-Dunghu, Justus J and 11 more authors.. (2008) EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase.. Journal of cell science, (15): [PMID:18445686] |
| 10. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 11. Garapaty, Shivani S and 5 more authors.. (2009) Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation.. The Journal of biological chemistry, (20): [PMID:19131338] |
| 12. Li, Zhenyu Z and 5 more authors.. (2009) Inhibition of SUV39H1 methyltransferase activity by DBC1.. The Journal of biological chemistry, (17): [PMID:19218236] |
| 13. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 14. Koyama, Satoshi S and 12 more authors.. (2010) Repression of estrogen receptor beta function by putative tumor suppressor DBC1.. Biochemical and biophysical research communications, (12): [PMID:20074560] |
| 15. Hiraike, H H and 17 more authors.. (2010) Identification of DBC1 as a transcriptional repressor for BRCA1.. British journal of cancer, (16): [PMID:20160719] |
| 16. Chini, Claudia C S CC, Escande, Carlos C, Nin, Veronica V and Chini, Eduardo N EN.. (2010) HDAC3 is negatively regulated by the nuclear protein DBC1.. The Journal of biological chemistry, (24): [PMID:21030595] |
| 17. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 18. Close, Pierre P and 9 more authors.. (2012) DBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation.. Nature, (25): [PMID:22446626] |
| 19. Zannini, Laura L, Buscemi, Giacomo G, Kim, Ja-Eun JE, Fontanella, Enrico E and Delia, Domenico D.. (2012) DBC1 phosphorylation by ATM/ATR inhibits SIRT1 deacetylase in response to DNA damage.. Journal of molecular cell biology, [PMID:22735644] |
| 20. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 21. Kim, Wootae W and Kim, Ja-Eun JE.. (2013) Deleted in breast cancer 1 (DBC1) deficiency results in apoptosis of breast cancer cells through impaired responses to UV-induced DNA damage.. Cancer letters, (10): [PMID:23352644] |
| 22. Chini, Claudia C S CC, Escande, Carlos C, Nin, Veronica V and Chini, Eduardo N EN.. (2013) DBC1 (Deleted in Breast Cancer 1) modulates the stability and function of the nuclear receptor Rev-erbα.. The Biochemical journal, (1): [PMID:23398316] |
| 23. Chini, Eduardo Nunes EN, Chini, Claudia C S CC, Nin, Veronica V and Escande, Carlos C.. (2013) Deleted in breast cancer-1 (DBC-1) in the interface between metabolism, aging and cancer.. Bioscience reports, (23): [PMID:23841676] |
| 24. Zheng, Hong H and 6 more authors.. (2013) hMOF acetylation of DBC1/CCAR2 prevents binding and inhibition of SirT1.. Molecular and cellular biology, [PMID:24126058] |
| 25. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 26. Joshi, P P, Quach, O L OL, Giguere, S S B SS and Cristea, I M IM.. (2013) A Functional Proteomics Perspective of DBC1 as a Regulator of Transcription.. Journal of proteomics & bioinformatics, (18): [PMID:24273392] |
| 27. Nin, Veronica V, Chini, Claudia C S CC, Escande, Carlos C, Capellini, Verena V and Chini, Eduardo N EN.. (2014) Deleted in breast cancer 1 (DBC1) protein regulates hepatic gluconeogenesis.. The Journal of biological chemistry, (28): [PMID:24415752] |
| 28. Pangon, Laurent L and 8 more authors.. (2015) MCC inhibits beta-catenin transcriptional activity by sequestering DBC1 in the cytoplasm.. International journal of cancer, (1): [PMID:24824780] |
| 29. Bae, Jun Sang JS and 12 more authors.. (2015) CK2α phosphorylates DBC1 and is involved in the progression of gastric carcinoma and predicts poor survival of gastric carcinoma patients.. International journal of cancer, (15): [PMID:24962073] |
| 30. Magni, Martina M and 7 more authors.. (2014) Chk2 and REGγ-dependent DBC1 regulation in DNA damage induced apoptosis.. Nucleic acids research, (1): [PMID:25361978] |
| 31. Park, Jong Ho JH and 9 more authors.. (2014) Modification of DBC1 by SUMO2/3 is crucial for p53-mediated apoptosis in response to DNA damage.. Nature communications, (18): [PMID:25406032] |
| 32. Sakurabashi, Ayako A and 12 more authors.. (2015) CCAR2 negatively regulates nuclear receptor LXRα by competing with SIRT1 deacetylase.. The Journal of steroid biochemistry and molecular biology, [PMID:25661920] |
| 33. Qin, Bo B and 9 more authors.. (2015) DBC1 functions as a tumor suppressor by regulating p53 stability.. Cell reports, (3): [PMID:25732823] |
| 34. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
