| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab169296-100μg | 100μg | In stock | $59.90 | |
| Ab169296-1mg | 1mg | In stock | $267.90 | |
| Ab169296-5mg | 5mg | In stock | $837.90 | |
| Ab169296-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,317.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Dupilumab (anti-IL-4Ra) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to IL-4R, >95%, high purity, Human IgG4SP |
|---|---|
| Synonyms | CD124 antibody | IL 4R alpha antibody | IL-4 receptor subunit alpha antibody | IL-4-binding protein antibody | IL-4R subunit alpha antibody | IL-4R-alpha antibody | IL-4RA antibody | IL4-BP antibody | Il4r antibody | IL4R nirs variant 1 antibody | IL4RA a |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Host species | Human |
| Specificity | IL-4R |
| Application | ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | ANTAGONIST |
| Mechanism of action | Antibody of Interleukin-4 receptor subunit α |
| Product Description | Dupilumab is a fully human mAb to IL-4 receptor α (IL-4Rα) that inhibits both IL-4 and IL-13 signaling, markedly improved moderate-to-severe atopic dermatitis. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Human IgG4SP |
| Light Chain Type | kappa |
| SDS-PAGE | 26.4 kDa (Light Chain) & 52.8 kDa (Heavy Chain), under reducing conditions; 187.4 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 1190264-60-8 |
Dupilumab (anti-IL-4Ra) (Ab169296) - Flow Cytometry
HEK293 human embryonic kidney cell line transfected with rhuIL-4R-full length was stained with Dupilumab (anti-IL-4Ra) (Ab169296).
Dupilumab (anti-IL-4Ra) (Ab169296) - SEC
The purity of Dupilumab (anti-IL-4Ra) (Ab169296) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303678 | Certificate of Analysis | Mar 25, 2024 | Ab169296 |
| ZJ24F0303677 | Certificate of Analysis | Mar 25, 2024 | Ab169296 |
| ZJ24F0303676 | Certificate of Analysis | Mar 25, 2024 | Ab169296 |
| 1. Wenzel S, Ford L, Pearlman D, Spector S, Sher L, Skobieranda F, Wang L, Kirkesseli S, Rocklin R, Bock B et al.. (2013) Dupilumab in persistent asthma with elevated eosinophil levels.. N Engl J Med, 368 (26): (2455-66). [PMID:23688323] [10.1021/op500134e] |
| 2. Murphy AJ, Macdonald LE, Stevens S, Karow M, Dore AT, Pobursky K, Huang TT, Poueymirou WT, Esau L, Meola M et al.. (2014) Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice.. Proc Natl Acad Sci USA, 111 (14): (5153-8). [PMID:24706856] [10.1021/op500134e] |
| 3. Bachert C, Mannent L, Naclerio RM, Mullol J, Ferguson BJ, Gevaert P, Hellings P, Jiao L, Wang L, Evans RR et al.. (2016) Effect of Subcutaneous Dupilumab on Nasal Polyp Burden in Patients With Chronic Sinusitis and Nasal Polyposis: A Randomized Clinical Trial.. JAMA, 315 (5): (469-79). [PMID:26836729] [10.1021/op500134e] |
| 4. Hershey GK, Friedrich MF, Esswein LA, Thomas ML, Chatila TA. (1997) The association of atopy with a gain-of-function mutation in the alpha subunit of the interleukin-4 receptor.. N Engl J Med, 337 (24): (1720-5). [PMID:9392697] [10.1021/op500134e] |
| 5. Galizzi, J P JP and 8 more authors.. (1990) Molecular cloning of a cDNA encoding the human interleukin 4 receptor.. International immunology, [PMID:2278997] |
| 6. Idzerda, R L RL and 9 more authors.. (1990) Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily.. The Journal of experimental medicine, (1): [PMID:2307934] |
| 7. Rolling, C C, Treton, D D, Beckmann, P P, Galanaud, P P and Richard, Y Y.. (1995) JAK3 associates with the human interleukin 4 receptor and is tyrosine phosphorylated following receptor triggering.. Oncogene, (4): [PMID:7538655] |
| 8. Zurawski, S M SM and 7 more authors.. (1995) The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor.. The Journal of biological chemistry, (9): [PMID:7775445] |
| 9. Keegan, A D AD and 5 more authors.. (1994) An IL-4 receptor region containing an insulin receptor motif is important for IL-4-mediated IRS-1 phosphorylation and cell growth.. Cell, (11): [PMID:8124718] |
| 10. Ryan, J J JJ and 7 more authors.. (1996) Growth and gene expression are predominantly controlled by distinct regions of the human IL-4 receptor.. Immunity, [PMID:8624803] |
| 11. Rolling, C C, Treton, D D, Pellegrini, S S, Galanaud, P P and Richard, Y Y.. (1996) IL4 and IL13 receptors share the gamma c chain and activate STAT6, STAT3 and STAT5 proteins in normal human B cells.. FEBS letters, (9): [PMID:8804422] |
| 12. Deichmann, K K, Bardutzky, J J, Forster, J J, Heinzmann, A A and Kuehr, J J.. (1997) Common polymorphisms in the coding part of the IL4-receptor gene.. Biochemical and biophysical research communications, (24): [PMID:9070874] |
| 13. Mitsuyasu, H H and 11 more authors.. (1998) Ile50Val variant of IL4R alpha upregulates IgE synthesis and associates with atopic asthma.. Nature genetics, [PMID:9620765] |
| 14. Wang, H Y HY and 5 more authors.. (1999) Cutting edge: effects of an allergy-associated mutation in the human IL-4R alpha (Q576R) on human IL-4-induced signal transduction.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:10201973] |
| 15. Hage, T T, Sebald, W W and Reinemer, P P.. (1999) Crystal structure of the interleukin-4/receptor alpha chain complex reveals a mosaic binding interface.. Cell, (16): [PMID:10219247] |
| 16. Kruse, S S and 6 more authors.. (1999) The polymorphisms S503P and Q576R in the interleukin-4 receptor alpha gene are associated with atopy and influence the signal transduction.. Immunology, [PMID:10233717] |
| 17. Jung, T T, Schrader, N N, Hellwig, M M, Enssle, K H KH and Neumann, C C.. (1999) Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases.. International archives of allergy and immunology, [PMID:10341317] |
| 18. Noguchi, E E and 9 more authors.. (1999) No association between atopy/asthma and the ILe50Val polymorphism of IL-4 receptor.. American journal of respiratory and critical care medicine, [PMID:10390422] |
| 19. Loftus, B J BJ and 18 more authors.. (1999) Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q.. Genomics, (15): [PMID:10493829] |
| 20. Kruse, S S, Forster, J J, Kuehr, J J and Deichmann, K A KA.. (1999) Characterization of the membrane-bound and a soluble form of human IL-4 receptor alpha produced by alternative splicing.. International immunology, [PMID:10590262] |
| 21. Ober, C C and 16 more authors.. (2000) Variation in the interleukin 4-receptor alpha gene confers susceptibility to asthma and atopy in ethnically diverse populations.. American journal of human genetics, [PMID:10677312] |
| 22. Oiso, N N, Fukai, K K and Ishii, M M.. (2000) Interleukin 4 receptor alpha chain polymorphism Gln551Arg is associated with adult atopic dermatitis in Japan.. The British journal of dermatology, [PMID:10809862] |
| 23. Lozano, F F and 9 more authors.. (2001) Identification of a novel single-nucleotide polymorphism (Val554Ile) and definition of eight common alleles for human IL4RA exon 11.. Tissue antigens, [PMID:11285129] |
| 24. Andrews, R P RP and 6 more authors.. (2001) Analysis of the Ser786Pro interleukin-4 receptor alpha allelic variant in allergic and nonallergic asthma and its functional consequences.. Clinical immunology (Orlando, Fla.), [PMID:11513543] |
| 25. Kashiwada, M M, Giallourakis, C C CC, Pan, P Y PY and Rothman, P B PB.. (2001) Immunoreceptor tyrosine-based inhibitory motif of the IL-4 receptor associates with SH2-containing phosphatases and regulates IL-4-induced proliferation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:11714803] |
| 26. Zhang, Jin-Li JL, Simeonowa, Irina I, Wang, Yonghong Y and Sebald, Walter W.. (2002) The high-affinity interaction of human IL-4 and the receptor alpha chain is constituted by two independent binding clusters.. Journal of molecular biology, (18): [PMID:11786020] |
| 27. Hanson, Erica M EM, Dickensheets, Harold H, Qu, Cheng-Kui CK, Donnelly, Raymond P RP and Keegan, Achsah D AD.. (2003) Regulation of the dephosphorylation of Stat6. Participation of Tyr-713 in the interleukin-4 receptor alpha, the tyrosine phosphatase SHP-1, and the proteasome.. The Journal of biological chemistry, (7): [PMID:12459556] |
| 28. Nakamura, Hiroyuki H and 10 more authors.. (2003) High contribution contrast between the genes of eosinophil peroxidase and IL-4 receptor alpha-chain in Japanese cedar pollinosis.. The Journal of allergy and clinical immunology, [PMID:14657871] |
| 29. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553] |
| 30. Malabarba, M G MG and 7 more authors.. (1995) Activation of JAK3, but not JAK1, is critical to interleukin-4 (IL4) stimulated proliferation and requires a membrane-proximal region of IL4 receptor alpha.. The Journal of biological chemistry, (21): [PMID:7721895] |
