mAb (7H12); Mouse anti Human E Cadherin Antibody; WB, IHC, Flow, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab100725-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab100725-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab100725-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab100725-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,237.90 |
mAb (7H12); Mouse anti Human E Cadherin Antibody; WB, IHC, Flow, ELISA; Unconjugated
| Product Name | E Cadherin Mouse mAb - Primary antibody, specific to E Cadherin, IgG1 |
|---|---|
| Synonyms | Arc 1 antibody; CADH1_HUMAN antibody; Cadherin 1 antibody; cadherin 1 type 1 E-cadherin antibody; Cadherin-1 antibody; Cadherin1 antibody; CAM 120/80 antibody; CD 324 antibody; CD324 antibody; CD324 antigen antibody; cdh1 antibody; CDHE antibody; E-Cad/CT |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | E Cadherin |
| Immunogen | Synthetic peptide of human CDH1 (AA: DWVIPPISCPENEKC) |
| Positive Control | WB: LNCAP, A431, DU145, PC-3, MCF-7, PC-12, NIH/3T3, C6 and COS7 cell lysates. IHC: Gastric cancer tissues and Lung cancer tissues. Flow: Hela cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse monoclonal (7H12) to E Cadherin, could be used for WB, IHC and Flow. |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles. |
E Cadherin Mouse mAb (Ab100725) - Western Blot
All lanes: E Cadherin Mouse mAb (Ab100725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 135 kDa
Observed band size: 112&124 kDa
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human gastric tissues using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - Flow Cytometry
Flow cytometric analysis of HeLa cells using E Cadherin Mouse mAb (Ab100725) (green) and negative control (purple).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| Flow |
1/200-1/400
|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700709 | Certificate of Analysis | Jul 26, 2023 | Ab100725 |
| ZJ23F0700712 | Certificate of Analysis | Jul 26, 2023 | Ab100725 |
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