| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab100725-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab100725-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab100725-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab100725-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,237.90 |
mAb (7H12); Mouse anti Human E Cadherin Antibody; WB, IHC, Flow, ELISA; Unconjugated
| Product Name | E Cadherin Mouse mAb - Primary antibody, specific to E Cadherin, IgG1 |
|---|---|
| Synonyms | Arc 1 antibody | CADH1_HUMAN antibody | Cadherin 1 antibody | cadherin 1 type 1 E-cadherin antibody | Cadherin-1 antibody | Cadherin1 antibody | CAM 120/80 antibody | CD 324 antibody | CD324 antibody | CD324 antigen antibody | cdh1 antibody | CDHE antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | E Cadherin |
| Immunogen | Synthetic peptide of human CDH1 (AA: DWVIPPISCPENEKC) |
| Positive Control | WB: LNCAP, A431, DU145, PC-3, MCF-7, PC-12, NIH/3T3, C6 and COS7 cell lysates. IHC: Gastric cancer tissues and Lung cancer tissues. Flow: Hela cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse monoclonal (7H12) to E Cadherin, could be used for WB, IHC and Flow. |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles. |
E Cadherin Mouse mAb (Ab100725) - Western Blot
All lanes: E Cadherin Mouse mAb (Ab100725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 135 kDa
Observed band size: 112&124 kDa
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human gastric tissues using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - Flow Cytometry
Flow cytometric analysis of HeLa cells using E Cadherin Mouse mAb (Ab100725) (green) and negative control (purple).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
">
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700709 | Certificate of Analysis | Jul 26, 2023 | Ab100725 |
| ZJ23F0700712 | Certificate of Analysis | Jul 26, 2023 | Ab100725 |
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| 20. Lecuit, M M and 5 more authors.. (1999) A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes.. The EMBO journal, (15): [PMID:10406800] |
| 21. Ito, K K and 8 more authors.. (1999) Calcium influx triggers the sequential proteolysis of extracellular and cytoplasmic domains of E-cadherin, leading to loss of beta-catenin from cell-cell contacts.. Oncogene, (25): [PMID:10597309] |
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| 23. Steinhusen, U U and 5 more authors.. (2001) Cleavage and shedding of E-cadherin after induction of apoptosis.. The Journal of biological chemistry, (16): [PMID:11076937] |
| 24. Baki, L L and 10 more authors.. (2001) Presenilin-1 binds cytoplasmic epithelial cadherin, inhibits cadherin/p120 association, and regulates stability and function of the cadherin/catenin adhesion complex.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:11226248] |
| 25. Salahshor, S S and 13 more authors.. (2001) A germline E-cadherin mutation in a family with gastric and colon cancer.. International journal of molecular medicine, [PMID:11562785] |
| 26. Ikonen, T T and 10 more authors.. (2001) Association of E-cadherin germ-line alterations with prostate cancer.. Clinical cancer research : an official journal of the American Association for Cancer Research, [PMID:11705864] |
| 27. Chen, Xinyu X, Bonne, Stefan S, Hatzfeld, Mechthild M, van Roy, Frans F and Green, Kathleen J KJ.. (2002) Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling.. The Journal of biological chemistry, (22): [PMID:11790773] |
| 28. Marambaud, Philippe P and 11 more authors.. (2002) A presenilin-1/gamma-secretase cleavage releases the E-cadherin intracellular domain and regulates disassembly of adherens junctions.. The EMBO journal, (15): [PMID:11953314] |
| 29. Oliveira, Carla C and 10 more authors.. (2002) Screening E-cadherin in gastric cancer families reveals germline mutations only in hereditary diffuse gastric cancer kindred.. Human mutation, [PMID:11968083] |
| 30. Meigs, Thomas E TE, Fedor-Chaiken, Mary M, Kaplan, Daniel D DD, Brackenbury, Robert R and Casey, Patrick J PJ.. (2002) Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin.. The Journal of biological chemistry, (5): [PMID:11976333] |
| 31. Yabuta, Tomonori T and 13 more authors.. (2002) E-cadherin gene variants in gastric cancer families whose probands are diagnosed with diffuse gastric cancer.. International journal of cancer, (10): [PMID:12216071] |
| 32. Schubert, Wolf Dieter WD and 8 more authors.. (2002) Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadherin.. Cell, (13): [PMID:12526809] |
| 33. Suriano, Gianpaolo G and 17 more authors.. (2003) Identification of CDH1 germline missense mutations associated with functional inactivation of the E-cadherin protein in young gastric cancer probands.. Human molecular genetics, (1): [PMID:12588804] |
| 34. Bharti, Sanita S and 5 more authors.. (2004) Novel membrane protein shrew-1 targets to cadherin-mediated junctions in polarized epithelial cells.. Molecular biology of the cell, [PMID:14595118] |
| 35. Laprise, Patrick P, Viel, Alain A and Rivard, Nathalie N.. (2004) Human homolog of disc-large is required for adherens junction assembly and differentiation of human intestinal epithelial cells.. The Journal of biological chemistry, (12): [PMID:14699157] |
| 36. Krakstad, Beate F BF, Ardawatia, Vandana V VV and Aragay, Anna M AM.. (2004) A role for Galpha12/Galpha13 in p120ctn regulation.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:15240885] |
| 37. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553] |
| 38. Lock, John G JG and Stow, Jennifer L JL.. (2005) Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin.. Molecular biology of the cell, [PMID:15689490] |
| 39. Serban, Geo G and 6 more authors.. (2005) Cadherins mediate both the association between PS1 and beta-catenin and the effects of PS1 on beta-catenin stability.. The Journal of biological chemistry, (28): [PMID:16126725] |
| 40. Agiostratidou, Georgia G, Muros, Rosa Miñana RM, Shioi, Junichi J, Marambaud, Philippe P and Robakis, Nikolaos K NK.. (2006) The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors.. Journal of neurochemistry, [PMID:16417575] |
| 41. Chanock, Stephen J SJ and 11 more authors.. (2007) Somatic sequence alterations in twenty-one genes selected by expression profile analysis of breast carcinomas.. Breast cancer research : BCR, [PMID:17224074] |
| 42. Ling, Kun K and 5 more authors.. (2007) Type I gamma phosphatidylinositol phosphate kinase modulates adherens junction and E-cadherin trafficking via a direct interaction with mu 1B adaptin.. The Journal of cell biology, (29): [PMID:17261850] |
| 43. Wollert, Thomas T and 8 more authors.. (2007) Extending the host range of Listeria monocytogenes by rational protein design.. Cell, (1): [PMID:17540170] |
| 44. Masciari, S S and 15 more authors.. (2007) Germline E-cadherin mutations in familial lobular breast cancer.. Journal of medical genetics, [PMID:17660459] |
| 45. Wollert, Thomas T, Heinz, Dirk W DW and Schubert, Wolf-Dieter WD.. (2007) Thermodynamically reengineering the listerial invasion complex InlA/E-cadherin.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17715295] |
| 46. Parisini, Emilio E, Higgins, Jonathan M G JM, Liu, Jin-huan JH, Brenner, Michael B MB and Wang, Jia-huai JH.. (2007) The crystal structure of human E-cadherin domains 1 and 2, and comparison with other cadherins in the context of adhesion mechanism.. Journal of molecular biology, (19): [PMID:17850815] |
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| 48. Abe, Kentaro K and Takeichi, Masatoshi M.. (2008) EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt.. Proceedings of the National Academy of Sciences of the United States of America, (8): [PMID:18093941] |
| 49. Zhou, Feng F and 9 more authors.. (2008) Unglycosylation at Asn-633 made extracellular domain of E-cadherin folded incorrectly and arrested in endoplasmic reticulum, then sequentially degraded by ERAD.. Glycoconjugate journal, [PMID:18491227] |
| 50. Pinho, Salomé S SS and 9 more authors.. (2009) The role of N-acetylglucosaminyltransferase III and V in the post-transcriptional modifications of E-cadherin.. Human molecular genetics, (15): [PMID:19403558] |
| 51. Li, Yili Y and 6 more authors.. (2009) Structure of natural killer cell receptor KLRG1 bound to E-cadherin reveals basis for MHC-independent missing self recognition.. Immunity, (17): [PMID:19604491] |
| 52. Frasa, Marieke A M MA and 10 more authors.. (2010) Armus is a Rac1 effector that inactivates Rab7 and regulates E-cadherin degradation.. Current biology : CB, (9): [PMID:20116244] |
| 53. Eswaramoorthy, Rajalakshmanan R and 7 more authors.. (2010) DDR1 regulates the stabilization of cell surface E-cadherin and E-cadherin-mediated cell aggregation.. Journal of cellular physiology, [PMID:20432435] |
| 54. Zhou, Wei-Jie WJ and 10 more authors.. (2011) Slit-Robo signaling induces malignant transformation through Hakai-mediated E-cadherin degradation during colorectal epithelial cell carcinogenesis.. Cell research, [PMID:21283129] |
| 55. Mukherjee, Manjeet M and 18 more authors.. (2012) Structure of a novel phosphotyrosine-binding domain in Hakai that targets E-cadherin.. The EMBO journal, (7): [PMID:22252131] |
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