pAb; Rabbit anti Human eIF-6 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101442-10μl | 10μl | In stock | $66.90 | |
| Ab101442-50μl | 50μl | In stock | $199.90 | |
| Ab101442-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab101442-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,779.90 |
pAb; Rabbit anti Human eIF-6 Antibody; WB, IHC; Unconjugated
| Product Name | eIF-6 Antibody - Primary antibody, specific to eIF-6, Rabbit IgG |
|---|---|
| Synonyms | CAB | EIF3A | ITGB4BP | b(2)gcn | eIF-6 | p27(BBP) | p27BBP | b(2)gcn antibody | B(2)GCN homolog antibody | B4 integrin interactor antibody | Binding protein of beta-4 integrin antibody | CAB antibody | eIF-6 antibody | EIF3A antibody | EIF6 antibody | Eu |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | eIF-6 |
| Immunogen | Fusion protein of human EIF6 (AA 38-245) |
| Positive Control | WB: A375, K562, HeLa, Hep G2 and HEK293 cell lysates. IHC: Human thyroid cancer tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human eIF-6 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
eIF-6 Antibody (Ab101442) - Western Blot
All lanes: eIF-6 Antibody (Ab101442) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 26 kDa
Exposure time: 52.9 s
eIF-6 Antibody (Ab101442) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using eIF-6 Antibody (Ab101442). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303344 | Certificate of Analysis | Mar 18, 2024 | Ab101442 |
| ZJ24F0303345 | Certificate of Analysis | Mar 18, 2024 | Ab101442 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] |
| 2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 3. Arunima Biswas,Shaeri Mukherjee,Supratik Das,Dennis Shields,Chi Wing Chow,Umadas Maitra. (2010-11-19) Opposing action of casein kinase 1 and calcineurin in nucleo-cytoplasmic shuttling of mammalian translation initiation factor eIF6.. The Journal of biological chemistry, 286 ((4)): (3129-3138). [PMID:21084295] |
| 4. Biffo, S S and 6 more authors.. (1997) Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells.. The Journal of biological chemistry, (28): [PMID:9374518] |
| 5. Si, K K, Chaudhuri, J J, Chevesich, J J and Maitra, U U.. (1997) Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:9405604] |
| 6. Mao, M M and 16 more authors.. (1998) Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:9653160] |
| 7. Sanvito, F F and 6 more authors.. (1999) The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly.. The Journal of cell biology, (8): [PMID:10085284] |
| 8. Donadini, A A, Giodini, A A, Sanvito, F F, Marchisio, P C PC and Biffo, S S.. (2001) The human ITGB4BP gene is constitutively expressed in vitro, but highly modulated in vivo.. Gene, (21): [PMID:11290417] |
| 9. Basu, Uttiya U, Si, Kausik K, Deng, Haiteng H and Maitra, Umadas U.. (2003) Phosphorylation of mammalian eukaryotic translation initiation factor 6 and its Saccharomyces cerevisiae homologue Tif6p: evidence that phosphorylation of Tif6p regulates its nucleocytoplasmic distribution and is required for yeast cell growth.. Molecular and cellular biology, [PMID:12917340] |
| 10. Ceci, Marcello M and 6 more authors.. (2003) Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly.. Nature, (4): [PMID:14654845] |
| 11. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 12. Chendrimada, Thimmaiah P TP and 7 more authors.. (2007) MicroRNA silencing through RISC recruitment of eIF6.. Nature, (14): [PMID:17507929] |
| 13. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 14. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 15. Finch, Andrew J AJ and 16 more authors.. (2011) Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.. Genes & development, (1): [PMID:21536732] |
