| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101333-10μl | 10μl | In stock | $69.90 | |
| Ab101333-50μl | 50μl | In stock | $199.90 | |
| Ab101333-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab101333-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,899.90 |
pAb; Rabbit anti Human EIF3H Antibody; WB, IHC; Unconjugated
| Product Name | EIF3H Antibody - Primary antibody, specific to EIF3H, Rabbit IgG |
|---|---|
| Synonyms | EIF3S3 | eIF3-gamma | eIF3-p40 | eIF-3-gamma antibody | eIF3 gamma antibody | eIF3 p40 antibody | eIF3 p40 subunit antibody | eIF3h antibody | EIF3H_HUMAN antibody | EIF3S3 antibody | Eukaryotic translation initiation factor 3 subunit 3 antibody | Eukaryo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | EIF3H |
| Immunogen | Fusion protein of human EIF3H (AA 80-340) |
| Positive Control | WB: Mouse spleen, A431 and C6 lysates. IHC: Human thyroid cancer tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human EIF3H Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
EIF3H Antibody (Ab101333) - Western Blot
All lanes: EIF3H Antibody (Ab101333) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 11.8 s
EIF3H Antibody (Ab101333) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using EIF3H Antibody (Ab101333). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303769 | Certificate of Analysis | Mar 27, 2024 | Ab101333 |
| ZJ24F0303768 | Certificate of Analysis | Mar 27, 2024 | Ab101333 |
| 1. Yandong Zhang,Jin You,Xingshun Wang,Jason Weber. (2015-06-24) The DHX33 RNA Helicase Promotes mRNA Translation Initiation.. Molecular and cellular biology, 35 ((17)): (2918-2931). [PMID:26100019] |
| 2. Asano, K K and 5 more authors.. (1997) Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.. The Journal of biological chemistry, (24): [PMID:9341143] |
| 3. Kolupaeva, Victoria G VG, Unbehaun, Anett A, Lomakin, Ivan B IB, Hellen, Christopher U T CU and Pestova, Tatyana V TV.. (2005) Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association.. RNA (New York, N.Y.), [PMID:15703437] |
| 4. Holz, Marina K MK, Ballif, Bryan A BA, Gygi, Steven P SP and Blenis, John J.. (2005) mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events.. Cell, (18): [PMID:16286006] |
| 5. Siridechadilok, Bunpote B, Fraser, Christopher S CS, Hall, Richard J RJ, Doudna, Jennifer A JA and Nogales, Eva E.. (2005) Structural roles for human translation factor eIF3 in initiation of protein synthesis.. Science (New York, N.Y.), (2): [PMID:16322461] |
| 6. LeFebvre, Aaron K AK and 7 more authors.. (2006) Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit.. The Journal of biological chemistry, (11): [PMID:16766523] |
| 7. Damoc, Eugen E and 8 more authors.. (2007) Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry.. Molecular & cellular proteomics : MCP, [PMID:17322308] |
| 8. Masutani, Mamiko M, Sonenberg, Nahum N, Yokoyama, Shigeyuki S and Imataka, Hiroaki H.. (2007) Reconstitution reveals the functional core of mammalian eIF3.. The EMBO journal, (25): [PMID:17581632] |
| 9. Zhou, Min M and 11 more authors.. (2008) Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:18599441] |
| 10. Lee, Jason P JP and 5 more authors.. (2010) The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.. Molecular cancer research : MCR, [PMID:20068067] |
| 11. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 12. Lee, Amy S Y AS, Kranzusch, Philip J PJ and Cate, Jamie H D JH.. (2015) eIF3 targets cell-proliferation messenger RNAs for translational activation or repression.. Nature, (4): [PMID:25849773] |
| 13. Lee, Amy S AS, Kranzusch, Philip J PJ, Doudna, Jennifer A JA and Cate, Jamie H D JH.. (2016) eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation.. Nature, (4): [PMID:27462815] |
| 14. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
