pAb; Rabbit anti Human EIF4E2 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101387-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab101387-50μl | 50μl | In stock | $199.90 | |
| Ab101387-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab101387-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human EIF4E2 Antibody; WB, IHC; Unconjugated
| Product Name | EIF4E2 Antibody - Primary antibody, specific to EIF4E2, Rabbit IgG |
|---|---|
| Synonyms | 4E LP; 4EHP; 4ELP; EIF 4E type 2; eIF-4E type 2; EIF4 E2; EIF4E 2; EIF4E HOMOLOGOUS PROTEIN; EIF4E like cap binding protein; EIF4E like protein 4E LP; EIF4E like protein 4ELP; EIF4E type 2; EIF4E type 3; eIF4E-like protein 4E-LP; EIF4EL3; Eukaryotic trans |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | EIF4E2 |
| Immunogen | Recombinant Human EIF4E2 Protein expressed in E.coli (AA 1-245) |
| Positive Control | WB: HEK293, Hep G2, HeLa, K562 and MCF7 cell lysates. IHC: Human stomach tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human EIF4E2 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified + Peptide Affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
EIF4E2 Antibody (Ab101387) - Western Blot
All lanes: EIF4E2 Antibody (Ab101387) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa
Exposure time: 9.5 s
EIF4E2 Antibody (Ab101387) - IHC
EIF4E2 Antibody (Ab101387) staining EIF4E2 in human stomach tissue sections by Immunohistochemistry. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with EIF4E2 Antibody (Ab101387) (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB |
1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202113 | Certificate of Analysis | Dec 20, 2023 | Ab101387 |
| ZJ23F1202112 | Certificate of Analysis | Dec 20, 2023 | Ab101387 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Louise von Stechow,Dimitris Typas,Jordi Carreras Puigvert,Laurens Oort,Ramakrishnaiah Siddappa,Alex Pines,Harry Vrieling,Bob van de Water,Leon H F Mullenders,Erik H J Danen. (2015-01-28) The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest.. Molecular and cellular biology, 35 ((7)): (1254-1268). [PMID:25624349] |
| 3. Rom, E E and 7 more authors.. (1998) Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.. The Journal of biological chemistry, (22): [PMID:9582349] |
| 4. Mao, M M and 16 more authors.. (1998) Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:9653160] |
| 5. Tan, Nancy G S NG and 5 more authors.. (2003) Human homologue of ariadne promotes the ubiquitylation of translation initiation factor 4E homologous protein, 4EHP.. FEBS letters, (20): [PMID:14623119] |
| 6. Joshi, Bhavesh B, Cameron, Amy A and Jagus, Rosemary R.. (2004) Characterization of mammalian eIF4E-family members.. European journal of biochemistry, [PMID:15153109] |
| 7. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 8. Okumura, Fumihiko F, Zou, Weiguo W and Zhang, Dong-Er DE.. (2007) ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP.. Genes & development, (1): [PMID:17289916] |
| 9. Rosettani, Pamela P, Knapp, Stefan S, Vismara, Maria-Grazia MG, Rusconi, Luisa L and Cameron, Alexander D AD.. (2007) Structures of the human eIF4E homologous protein, h4EHP, in its m7GTP-bound and unliganded forms.. Journal of molecular biology, (4): [PMID:17368478] |
| 10. Morita, Masahiro M and 16 more authors.. (2012) A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development.. Molecular and cellular biology, [PMID:22751931] |
| 11. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 12. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 13. Kubacka, Dorota D and 5 more authors.. (2013) Investigating the consequences of eIF4E2 (4EHP) interaction with 4E-transporter on its cellular distribution in HeLa cells.. PloS one, [PMID:23991149] |
| 14. Chapat, Clément C and 12 more authors.. (2017) Cap-binding protein 4EHP effects translation silencing by microRNAs.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:28487484] |
