ENO1 Mouse mAb - Primary antibody, specific to ENO1, Mouse IgG1

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Cow,Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: C2
  • Conjugation: Unconjugated
Item Number
Ab101688
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab101688-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab101688-50μl
50μl
In stock
$179.90
Ab101688-100μl
100μl
In stock
$279.90
Ab101688-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

mAb (C2); Mouse anti Human ENO1 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameENO1 Mouse mAb - Primary antibody, specific to ENO1, Mouse IgG1
Synonyms2 phospho D glycerate hydro lyase antibody | 2-phospho-D-glycerate hydro-lyase antibody | Alpha enolase antibody | Alpha enolase like 1 antibody | Alpha-enolase antibody | C myc promoter binding protein antibody | C-myc promoter-binding protein antibody |
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host speciesMouse
SpecificityENO1
ImmunogenRecombinant ENO1 expressed in E.coli (AA 1-434)
Positive ControlWB: HeLa, MCF7, A549, THP-1, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High Performance, Validated
Note20μl卖完停产,不再备货
Product Description

Mouse anti Human ENO1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-20μg/mL

Protein Function

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ENO1 Mouse mAb (Ab101688) - Western Blot
All lanes: ENO1 Mouse mAb (Ab101688) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 48 kDa
Exposure time: 2.5 s

ENO1 Mouse mAb (Ab101688) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

ENO1 Mouse mAb (Ab101688) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets(Human)

ENO1 Tchem Alpha-enolase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101820Certificate of AnalysisNov 27, 2023 Ab101688
ZJ23F1101817Certificate of AnalysisNov 27, 2023 Ab101688

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861] [10.1021/op500134e]
2. He Huang,Shuang Tang,Ming Ji,Zhanyun Tang,Miho Shimada,Xiaojing Liu,Shankang Qi,Jason W Locasale,Robert G Roeder,Yingming Zhao,Xiaoling Li.  (2018-05-19)  p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis..  Molecular cell,  70  ((4)): (663-678).  [PMID:29775581]
3. Yan VC, Yang KL, Ballato ES, Khadka S, Shrestha P, Arthur K, Georgiou DK, Washington M, Tran T, Poral AH, Pham CD, Yan MJ, Muller FL..  (2020)  Bioreducible Phosphonoamidate Pro-drug Inhibitor of Enolase: Proof of Concept Study..  ACS Med Chem Lett,  11  (7): (1484-1489).  [PMID:32676158] [10.1021/acsmedchemlett.0c00203]
4. Sugahara, T T, Nakajima, H H, Shirahata, S S and Murakami, H H..  (1992)  Purification and characterization of immunoglobulin production stimulating factor-II beta derived from Namalwa cells..  Cytotechnology,      [PMID:1369209]
5. Ray, R R and Miller, D M DM..  (1991)  Cloning and characterization of a human c-myc promoter-binding protein..  Molecular and cellular biology,      [PMID:2005901]
6. Giallongo, A A and 5 more authors..  (1990)  Structure of the human gene for alpha-enolase..  European journal of biochemistry,    (5):   [PMID:2373081]
7. Giallongo, A A, Feo, S S, Moore, R R, Croce, C M CM and Showe, L C LC..  (1986)  Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3529090]
8. Mohammad, R M RM, Hamdan, M Y MY and al-Katib, A A..  ()  Induced expression of alpha-enolase in differentiated diffuse large cell lymphoma..  Enzyme & protein,      [PMID:7787969]
9. Walter, M M, Berg, H H, Leidenberger, F A FA, Schweppe, K W KW and Northemann, W W..  (1995)  Autoreactive epitopes within the human alpha-enolase and their recognition by sera from patients with endometriosis..  Journal of autoimmunity,      [PMID:8824716]
10. Yu, W W and 9 more authors..  (1997)  Large-scale concatenation cDNA sequencing..  Genome research,      [PMID:9110174]
11. Ji, H H and 5 more authors..  ()  A two-dimensional gel database of human colon carcinoma proteins..  Electrophoresis,      [PMID:9150948]
12. Arza, B B, Félez, J J, Lopez-Alemany, R R, Miles, L A LA and Muñoz-Cánoves, P P..  (1997)  Identification of an epitope of alpha-enolase (a candidate plasminogen receptor) by phage display..  Thrombosis and haemostasis,      [PMID:9308760]
13. Onyango, P P, Lubyova, B B, Gardellin, P P, Kurzbauer, R R and Weith, A A..  (1998)  Molecular cloning and expression analysis of five novel genes in chromosome 1p36..  Genomics,    (1):   [PMID:9653645]
14. Adamus, G G, Amundson, D D, Seigel, G M GM and Machnicki, M M..  (1998)  Anti-enolase-alpha autoantibodies in cancer-associated retinopathy: epitope mapping and cytotoxicity on retinal cells..  Journal of autoimmunity,      [PMID:9878089]
15. Ghosh, A K AK, Steele, R R and Ray, R B RB..  (1999)  Functional domains of c-myc promoter binding protein 1 involved in transcriptional repression and cell growth regulation..  Molecular and cellular biology,      [PMID:10082554]
16. Subramanian, A A and Miller, D M DM..  (2000)  Structural analysis of alpha-enolase. Mapping the functional domains involved in down-regulation of the c-myc protooncogene..  The Journal of biological chemistry,    (25):   [PMID:10681589]
17. Feo, S S, Arcuri, D D, Piddini, E E, Passantino, R R and Giallongo, A A..  (2000)  ENO1 gene product binds to the c-myc promoter and acts as a transcriptional repressor: relationship with Myc promoter-binding protein 1 (MBP-1)..  FEBS letters,    (4):   [PMID:10802057]
18. Ghosh, A K AK, Majumder, M M, Steele, R R, White, R A RA and Ray, R B RB..  (2001)  A novel 16-kilodalton cellular protein physically interacts with and antagonizes the functional activity of c-myc promoter-binding protein 1..  Molecular and cellular biology,      [PMID:11134351]
19. and Pancholi, V V..  (2001)  Multifunctional alpha-enolase: its role in diseases..  Cellular and molecular life sciences : CMLS,      [PMID:11497239]
20. Ochi, Hirofumi H and 14 more authors..  (2002)  Proteomic analysis of human brain identifies alpha-enolase as a novel autoantigen in Hashimoto's encephalopathy..  FEBS letters,    (25):   [PMID:12297304]
21. López-Alemany, Roser R and 8 more authors..  (2003)  Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-Enolase..  American journal of hematology,      [PMID:12666133]
22. She, Yi-Min YM and 5 more authors..  (2003)  Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry..  Molecular & cellular proteomics : MCP,      [PMID:14534351]
23. Rush, John J and 9 more authors..  (2005)  Immunoaffinity profiling of tyrosine phosphorylation in cancer cells..  Nature biotechnology,      [PMID:15592455]
24. Giannakopoulos, Nadia V NV and 9 more authors..  (2005)  Proteomic identification of proteins conjugated to ISG15 in mouse and human cells..  Biochemical and biophysical research communications,    (21):   [PMID:16139798]
25. Leong, Wai Fook WF and Chow, Vincent T K VT..  (2006)  Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection..  Cellular microbiology,      [PMID:16548883]
26. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,    (18):   [PMID:16710414]
27. Wong, Joyce Jing Yi JJ, Pung, Yuh Fen YF, Sze, Newman Siu-Kwan NS and Chin, Keh-Chuang KC..  (2006)  HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets..  Proceedings of the National Academy of Sciences of the United States of America,    (11):   [PMID:16815975]
28. Bechtel, Stephanie S and 18 more authors..  (2007)  The full-ORF clone resource of the German cDNA Consortium..  BMC genomics,    (31):   [PMID:17974005]
29. Kang, Hyo Jin HJ, Jung, Suk-Kyeong SK, Kim, Seung Jun SJ and Chung, Sang J SJ..  (2008)  Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme..  Acta crystallographica. Section D, Biological crystallography,      [PMID:18560153]
30. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
31. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
32. Peng, Chao C and 18 more authors..  (2011)  The first identification of lysine malonylation substrates and its regulatory enzyme..  Molecular & cellular proteomics : MCP,      [PMID:21908771]
33. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,    (7):   [PMID:22905912]
34. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
35. Leonard, Paul G PG and 17 more authors..  (2016)  SF2312 is a natural phosphonate inhibitor of enolase..  Nature chemical biology,      [PMID:27723749]
36. Burr, Marian L ML and 23 more authors..  (2017)  CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity..  Nature,    (7):   [PMID:28813417]
37. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

Solution Calculators