ErbB2/Her2 Mouse mAb - Primary antibody, specific to ERBB2, Mouse IgG1
Synonyms
ERBB2 | HER2 | MLN19 | NEU | NGL | Receptor tyrosine-protein kinase erbB-2 | Metastatic lymph node gene 19 protein | MLN 19 | Proto-oncogene Neu | Proto-oncogene c-ErbB-2 | Tyrosine kinase-type cell surface receptor HER2 | p185erbB2 | CD antigen CD340 | V
Specifications & Purity
ExactAb™, Validated, 1mg/mL
Host species
Mouse
Specificity
ERBB2
Immunogen
A synthetic peptide derived from human HER2 (AA 1094-1111).
Positive Control
WB:Hela, A431, MCF-7 lysates IHC: Human Tonsil tissue
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Product Description
Mouse anti Human ErbB 2 Antibody, Monoclonal(1C4-1H3-1H3), could be used for WB, IHC and so on . Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
1mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
ErbB2Her2 Mouse mAb (Ab102061) - Western Blot All lanes: ErbB2Her2 Mouse mAb (Ab102061) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 185 kDa Observed band size: 181 kDa Exposure time: 10.0 s
ErbB2Her2 Mouse mAb (Ab102061) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using ErbB2Her2 Mouse mAb (Ab102061). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.