ErbB2/Her2 Mouse mAb - Primary antibody, specific to ERBB2, Mouse IgG1

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 1C4-1H3-1H3
  • Conjugation: Unconjugated
Item Number
Ab102061
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab102061-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab102061-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$177.90
Ab102061-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab102061-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,157.90

mAb(1C4-1H3-1H3); Mouse anti Human ErbB2 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameErbB2/Her2 Mouse mAb - Primary antibody, specific to ERBB2, Mouse IgG1
SynonymsERBB2 | HER2 | MLN19 | NEU | NGL | Receptor tyrosine-protein kinase erbB-2 | Metastatic lymph node gene 19 protein | MLN 19 | Proto-oncogene Neu | Proto-oncogene c-ErbB-2 | Tyrosine kinase-type cell surface receptor HER2 | p185erbB2 | CD antigen CD340 | V
Specifications & PurityExactAb™, Validated, 1mg/mL
Host speciesMouse
SpecificityERBB2
ImmunogenA synthetic peptide derived from human HER2 (AA 1094-1111).
Positive ControlWB:Hela, A431, MCF-7 lysates IHC: Human Tonsil tissue
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Mouse anti Human ErbB 2 Antibody, Monoclonal(1C4-1H3-1H3), could be used for WB, IHC and so on .
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ErbB2Her2 Mouse mAb (Ab102061) - Western Blot
All lanes: ErbB2Her2 Mouse mAb (Ab102061) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 185 kDa
Observed band size: 181 kDa
Exposure time: 10.0 s

ErbB2Her2 Mouse mAb (Ab102061) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using ErbB2Her2 Mouse mAb (Ab102061). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

ERBB2 Tclin Receptor tyrosine-protein kinase erbB-2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
ERBB2 Tclin Receptor protein-tyrosine kinase erbB-2 (7851 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ERBB2 Tclin Epidermal growth factor receptor and ErbB2 (HER1 and HER2) (186 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ERBB2 Tclin Epidermal growth factor receptor (487 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ERBB2 Tclin FASN/HER2 (2 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800859Certificate of AnalysisAug 22, 2023 Ab102061
ZJ23F0800858Certificate of AnalysisAug 22, 2023 Ab102061

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