Ethidium bromide(EB) - for molecular biology,powder,≥95% (HPLC), high purity , CAS No.1239-45-8

Item Number

E119045

• Synonyms: ETHIDIUM BROMIDE | Homidium bromide | 1239-45-8 | Dromilac | EtBr | 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide | 3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide | Ethydium bromide | 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide | 2,7-Diamino-9-phe
• Specifications & Purity: for molecular biology, ≥95%(HPLC), powder
• Biochemical and Physiological Mechanisms: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
• Storage Temp: Store at 2-8°C,Protected from light
• Shipped In: Wet ice
• Grade: for molecular biology

Product Description

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
Ethidium bromide is an aromatic cationic dye, is one of the most studied DNA-intercalating agents and has been used widely as a molecular probe. It is also used in the prophylaxis and treatment of animal trypanosomiasis in affected areas worldwide.