| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| E119045-1g | 1g | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $103.90 | |
| E119045-5g | 5g | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $343.90 | |
| E119045-25g | 25g | 3 | $1,373.90 |
| Synonyms | EB | 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide | CCG-39365 | CS-0009917 | Dromilac | Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1) | UNII-059NUO2Z1L | Homidium bromide | RD 1572 | Ethidium bromide, ~95% (HPLC) | NCGC00095070-0 |
|---|---|
| Specifications & Purity | for molecular biology, ≥95%(HPLC), powder |
| Biochemical and Physiological Mechanisms | Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| Storage Temp | Store at 2-8°C,Protected from light |
| Shipped In | Wet ice |
| Grade | 适用于分子生物学 |
| Product Description | For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis. |
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| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Pubchem Sid | 504752579 |
|---|---|
| Pubchem Sid Url | https://pubchem.ncbi.nlm.nih.gov/substance/504752579 |
| IUPAC Name | 5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine;bromide |
| INCHI | InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H |
| InChi Key | ZMMJGEGLRURXTF-UHFFFAOYSA-N |
| Canonical SMILES | CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-] |
| Isomeric SMILES | CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-] |
| WGK Germany | 3 |
| RTECS | SF7950000 |
| PubChem CID | 14710 |
| Molecular Weight | 394.32 |
Find and download the COA for your product by matching the lot number on the packaging.
13 results found
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 J2213143 | Certificate of Analysis | Oct 18, 2022 | E119045 |
| C2222027 | Certificate of Analysis | May 28, 2022 | E119045 |
| B2328186 | Certificate of Analysis | May 25, 2022 | E119045 |
| B2508058 | Certificate of Analysis | May 25, 2022 | E119045 |
| F2222070 | Certificate of Analysis | May 25, 2022 | E119045 |
| F2222073 | Certificate of Analysis | May 25, 2022 | E119045 |
| F2222074 | Certificate of Analysis | May 25, 2022 | E119045 |
| G2320190 | Certificate of Analysis | May 25, 2022 | E119045 |
| I2321078 | Certificate of Analysis | May 25, 2022 | E119045 |
| J2323058 | Certificate of Analysis | May 25, 2022 | E119045 |
| Sensitivity | heat & light sensitive |
|---|---|
| Melt Point(°C) | 260-262°C |
| Molecular Weight | 394.300 g/mol |
| XLogP3 | |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 3 |
| Rotatable Bond Count | 2 |
| Exact Mass | 393.084 Da |
| Monoisotopic Mass | 393.084 Da |
| Topological Polar Surface Area | 55.900 Ų |
| Heavy Atom Count | 25 |
| Formal Charge | 0 |
| Complexity | 419.000 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| The total count of all stereochemical bonds | 0 |
| Covalently-Bonded Unit Count | 2 |
| Pictogram(s) | GHS06, GHS08 |
|---|---|
| Signal | Danger |
| Hazard Statements | H341:Suspected of causing genetic defects H302:Harmful if swallowed H330:Fatal if inhaled |
| Precautionary Statements | P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P260:Do not breathe dust/fume/gas/mist/vapors/spray. P284:[In case of inadequate ventilation] Wear respiratory protection. P281:Use personal protective equipment as required. P271:Use only outdoors or in a well-ventilated area. P270:Do not eat, drink or smoke when using this product. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P330:Rinse mouth. P320:Specific treatment is urgent (see ... on this label). P203:Obtain, read and follow all safety instructions before use. P301+P317:IF SWALLOWED: Get medical help. P318:if exposed or concerned, get medical advice. P316:Get emergency medical help immediately. |
| WGK Germany | 3 |
| RTECS | SF7950000 |
| Merck Index | 4731 |
| 1. Yu Cao, Yiming Zhang, Chengyu Han, Shuo Liu, Shaojie Zhang, Xinyi Liu, Baoshan Zhang, Fusheng Pan, Jie Sun. (2023) Zwitterionic Covalent Organic Framework Based Electrostatic Field Electrocatalysts for Durable Lithium–Sulfur Batteries. ACS Nano, 17 (22): (22632–22641). [PMID:37933557] [10.1021/acsnano.3c06826] |
| 2. Jian Sun, Huayi Qu, Waseem Ali, Yan Chen, Tao Wang, Yonggang Ma, Yan Yuan, Jianhong Gu, Jianchun Bian, Zongping Liu, Hui Zou. (2023) Co-exposure to cadmium and microplastics promotes liver fibrosis through the hemichannels -ATP-P2X7 pathway. CHEMOSPHERE, (17): (140372). [PMID:37802476] [10.1016/j.chemosphere.2023.140372] |
| 3. Yufang Li, Xiaozhao Tang, Zushun Yang, Jinze He, Ni Ma, Aixiang Huang, Yanan Shi. (2023) BCp12/PLA combination: A novel antibacterial agent targeting Mur family, DNA gyrase and DHFR. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 406 (110370). [PMID:37678070] [10.1016/j.ijfoodmicro.2023.110370] |
| 4. Xiaolong Peng, Qiang Tang, Huiyun Zhu, Lijuan Bai, Hua Zhao, Yongjie Chen. (2023) Study on antitumor activity of three ruthenium arene complexes in vitro. JOURNAL OF INORGANIC BIOCHEMISTRY, 247 (112310). [PMID:37441921] [10.1016/j.jinorgbio.2023.112310] |
| 5. Ji Ma, Guoxia Huang, Changxin Mo, Junsheng Li, Liujuan Yan, Qian Zhang. (2023) Insights into the intercalative binding of benzo[b]fluoranthene with herring sperm DNA in vitro and its application. JOURNAL OF MOLECULAR LIQUIDS, 378 (121628). [PMID:] [10.1016/j.molliq.2023.121628] |
| 6. Zhixiao Liu, Zhiming Mi, Lingjun Meng, Yangyang Huang, Dexing Zhang, Junman Wang, Kaiyang Zhang, Jingling Xiao, Pingli Liu, Zhi Rao, Hongru He, Shuai Wang. (2023) Quaternary ammonium salts modification preparing charged Janus nanofiltration membrane for the simultaneous separation of divalent anions and cations. JOURNAL OF MEMBRANE SCIENCE, 672 (121440). [PMID:] [10.1016/j.memsci.2023.121440] |
| 7. Jing Yang, Wen Ting Wang, Zheng Dou Shi, Rui Yang, Xia Li Liao, Bo Yang, Chuan Zhu Gao. (2022) Phosphorescent cyclometalated iridium(III) complexes with mono-, di-, and tetranuclear as two channel probes to light up the nuclei of living cells. APPLIED ORGANOMETALLIC CHEMISTRY, 37 (2): (e6964). [PMID:] [10.1002/aoc.6964] |
| 8. Juan Wu, Xi Wang, Binglong Zhu, Qinting He, Yaheng Zhang, Wei Jiang. (2022) Synthesis and characterization of magnetic polymeric nanocomposites for pH-sensitive controlled release of methotrexate. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, [PMID:35727073] [10.1080/09205063.2022.2093053] |
| 9. Lujun Yin, Ping-Ping Zhang, Wei Wang, Shi Tang, Shi-Ming Deng, Ai-Qun Jia. (2022) 3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing. Microbiology Spectrum, [PMID:35972277] [10.1128/spectrum.01829-22] |
| 10. Liangping Li, Pingping Li, Xuesong Ma, Shulan Zeng, Yan Peng, Guohai Zhang. (2022) Therapeutic restoring p53 function with small molecule for oncogene-driven non-small cell lung cancer by targeting serine 392 phosphorylation. BIOCHEMICAL PHARMACOLOGY, 203 (115188). [PMID:35902040] [10.1016/j.bcp.2022.115188] |
| 11. Wei-Hao Wu, Xiao-Mei Ma, Jian-Qing Huang, Qin Lai, Fu-Neng Jiang, Cui-Yun Zou, Long-Tian Chen, Lian Yu. (2022) CRISPR/Cas9 (D10A) nickase-mediated Hb CS gene editing and genetically modified fibroblast identification. Bioengineered, 13 (5): (13398-13406). [PMID:36700476] [10.1080/21655979.2022.2069940] |
| 12. Lihua Chen, Pan Zhao, Jing Yang, Xiali Liao, Bo Yang, Chuanzhu Gao. (2022) Synthesis, characterization, and antitumor biological evaluation of novel fluorine-containing platinum (IV) complexes. APPLIED ORGANOMETALLIC CHEMISTRY, 36 (7): (e6759). [PMID:] [10.1002/aoc.6759] |
| 13. Huang Yiqian, Du Zhiyun, Li Ke, Jing Wei, Wei Pengfei, Zhao Bo, Yu Yingjie, Cai Qing, Yang Xiaoping. (2022) ROS-Scavenging Electroactive Polyphosphazene-Based Core–Shell Nanofibers for Bone Regeneration. Advanced Fiber Materials, 4 (4): (894-907). [PMID:] [10.1007/s42765-022-00153-8] |
| 14. Haolu Hu, Xiaoxia Kang, Zhenhua Shan, Xiaoxiao Yang, Wei Bing, Li Wu, Haiyan Ge, Haiwei Ji. (2022) A DNase-mimetic artificial enzyme for the eradication of drug-resistant bacterial biofilm infections. Nanoscale, 14 (7): (2676-2685). [PMID:35107481] [10.1039/D1NR07629A] |
| 15. Jinmin Wang, Xin Xu, Limin Zhao, Bing Yan. (2021) In situ growth of polyoxometalate in COF for trace monitoring of Ag+ and hepatocellular carcinoma biomarker via a dual responsive strategy. Journal of Materials Chemistry C, 9 (30): (9492-9498). [PMID:] [10.1039/D1TC01938D] |
| 16. Feng Xiao, Jiayu Liu, Yongbo Zheng, Zhen Quan, Wei Sun, Yao Fan, Chunli Luo, Hailiang Li, Xiaohou Wu. (2020) The targeted inhibition of prostate cancer by iron-based nanoparticles based on bioinformatics. JOURNAL OF BIOMATERIALS APPLICATIONS, [PMID:33283584] [10.1177/0885328220975249] |
| 17. Zhaohui Chen, Shuihua Wu. (2020) Protective Effects of SIRT6 Against Inflammation, Oxidative Stress, and Cell Apoptosis in Spinal Cord Injury. INFLAMMATION, 43 (5): (1751-1758). [PMID:32445068] [10.1007/s10753-020-01249-2] |
| 18. Kaiyao Shi, Yang Liu, Jingru Ma, Yuqing Zhao, Liang Liu, Jingying Wang. (2020) A series of aromatric-amine-cored fluorescent emitters for amplified spontaneous laser emission and emission turn-on sensing. JOURNAL OF LUMINESCENCE, 223 (117165). [PMID:] [10.1016/j.jlumin.2020.117165] |
| 19. Zeng Xin, Zhu Min, Liu Xiaohong, Chen Xuanmin, Yuan Yujia, Li Lan, Liu Jingping, Lu Yanrong, Cheng Jingqiu, Chen Younan. (2020) Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis. Nutrition & Metabolism, 17 (1): (1-14). [PMID:32021639] [10.1186/s12986-020-0434-8] |
| 20. Haixia Liu, Qingzhu Yang, Wei Guo, Huiming Lin, Feng Zhang, Jingxiang Zhao, Tianyue Ma, Le Zhao, Na Xu, Ruiwen Wang, Jingyao Yu, Fengyu Qu. (2020) CoWO4-x-based nanoplatform for multimode imaging and enhanced photothermal/photodynamic therapy. CHEMICAL ENGINEERING JOURNAL, 385 (123979). [PMID:] [10.1016/j.cej.2019.123979] |
| 21. Yun Li, Yueqin Li, Nana Wang, Dong Lin, Xiaohui Liu, Yong Yang, Qinwei Gao. (2020) Synthesis, DNA/BSA binding studies and in vitro biological assay of nickel(II) complexes incorporating tridentate aroylhydrazone and triphenylphosphine ligands. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 38 (17): (4977-4996). [PMID:31739745] [10.1080/07391102.2019.1694995] |
| 22. Haiming Yu, Yuefei Wang, Xiaoqing Wang, Junjie Guo, Hui Wang, Hao Zhang, Fengxia Du. (2019) Jatrorrhizine suppresses the antimicrobial resistance of methicillin‑resistant Staphylococcus aureus. Experimental and Therapeutic Medicine, 18 (5): (3715-3722). [PMID:31616505] [10.3892/etm.2019.8034] |
| 23. Rui Wang, Xing Hu, Junhui Pan, Guowen Zhang, Deming Gong. (2019) Interaction of isoeugenol with calf thymus DNA and its protective effect on DNA oxidative damage. JOURNAL OF MOLECULAR LIQUIDS, 282 (356). [PMID:] [10.1016/j.molliq.2019.03.018] |
| 24. Zhisheng Zhou, Xing Hu, Guowen Zhang, Rui Wang, Deming Gong. (2019) Exploring the binding interaction of Maillard reaction by-product 5-hydroxymethyl-2-furaldehyde with calf thymus DNA. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 99 (6): (3192-3202). [PMID:30548611] [10.1002/jsfa.9536] |
| 25. Xunan Jing, Zhe Zhi, Daquan Wang, Jing Liu, Yongping Shao, Lingjie Meng. (2018) Multifunctional Nanoflowers for Simultaneous Multimodal Imaging and High-Sensitivity Chemo-Photothermal Treatment. BIOCONJUGATE CHEMISTRY, 29 (2): (559–570). [PMID:29376319] [10.1021/acs.bioconjchem.8b00053] |
| 26. Shi-Ying Li, Hong Cheng, Bo-Ru Xie, Wen-Xiu Qiu, Chu-Xin Li, Bin Li, Han Cheng, Xian-Zheng Zhang. (2017) Mitochondria targeted cancer therapy using ethidium derivatives. Materials Today Chemistry, 6 (34). [PMID:] [10.1016/j.mtchem.2017.09.002] |
| 27. Ramachandran Vinayagam, Baojun Xu. (2017) 7, 8-Dihydroxycoumarin (daphnetin) protects INS-1 pancreatic β-cells against streptozotocin-induced apoptosis. PHYTOMEDICINE, 24 (119). [PMID:28160851] [10.1016/j.phymed.2016.11.023] |
| 28. Juan Wu, Wei Jiang, Yewen Shen, Wei Jiang, Renbing Tian. (2017) Synthesis and characterization of mesoporous magnetic nanocomposites wrapped with chitosan gatekeepers for pH-sensitive controlled release of doxorubicin. Materials Science & Engineering C-Materials for Biological Applications, 70 (132). [PMID:27770872] [10.1016/j.msec.2016.08.054] |
| 29. Zhaohui Yang, Min Li, Mingda Yu, Jing Huang, Haiyin Xu, Yan Zhou, Peipei Song, Rui Xu. (2016) A novel approach for methylene blue removal by calcium dodecyl sulfate enhanced precipitation and microbial flocculant GA1 flocculation. CHEMICAL ENGINEERING JOURNAL, 303 (1). [PMID:] [10.1016/j.cej.2016.05.101] |
| 30. Hailiang Huang, Shuo Shi, Xuyue Zheng, Tianming Yao. (2015) Sensitive detection for coralyne and mercury ions based on homo-A/T DNA by exonuclease signal amplification. BIOSENSORS & BIOELECTRONICS, 71 (439). [PMID:25950941] [10.1016/j.bios.2015.04.076] |
| 31. Jinhua Zhu, Lanlan Chen, Yingying Dong, Jiazhong Li, Xiuhua Liu. (2014) Spectroscopic and molecular modeling methods to investigate the interaction between 5-Hydroxymethyl-2-furfural and calf thymus DNA using ethidium bromide as a probe. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 124 (78). [PMID:24463243] [10.1016/j.saa.2013.12.091] |
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