Determine the necessary mass, volume, or concentration for preparing a solution.
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SKU | Size | Availability | Price | Qty |
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F109384-1g | 1g | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $14.90 | |
F109384-5g | 5g | In stock | $58.90 | |
F109384-25g | 25g | In stock | $265.90 | |
F109384-100g | 100g | In stock | $955.90 |
Green fluorogenic substrate for esterase activity
Synonyms | Fluorescein diacetate | 596-09-8 | Diacetylfluorescein | 3,6-Diacetoxyfluoran | di-O-Acetylfluorescein | 3',6'-Diacetylfluorescein | Fluorescein 3',6'-diacetate | Fluorescein, diacetate | NSC-4726 | NSC-667259 | MLS000722997 | MFCD-5062 | (6'-acetyloxy-3-oxospiro[2-benzofuran-1, |
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Specifications & Purity | ≥97% |
Biochemical and Physiological Mechanisms | Widely-used fluorogenic\xa0substrate for esterase activity. Upon cleavage fluorescein is released producing a green fluorescence that can be read using standard FITC filter sets. Common viability stain for plant cells, protoplasts and tissues. |
Storage Temp | Store at 2-8°C,Argon charged |
Shipped In | Wet ice |
Note | Refer to SDS for further information. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details. |
Product Description | Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. |
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IUPAC Name | (6'-acetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate |
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INCHI | InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3 |
InChi Key | CHADEQDQBURGHL-UHFFFAOYSA-N |
Canonical SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC=CC=C5C(=O)O3 |
Isomeric SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC=CC=C5C(=O)O3 |
WGK Germany | 3 |
PubChem CID | 65047 |
Molecular Weight | 416.38 |
Beilstein | 62575 |
Reaxy-Rn | 62575 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
Lot Number | Certificate Type | Date | Item |
---|---|---|---|
K2224113 | Certificate of Analysis | Nov 25, 2022 | F109384 |
H2204040 | Certificate of Analysis | Jun 17, 2022 | F109384 |
H2204041 | Certificate of Analysis | Jun 17, 2022 | F109384 |
H2204042 | Certificate of Analysis | Jun 17, 2022 | F109384 |
H2204043 | Certificate of Analysis | Jun 17, 2022 | F109384 |
I2315027 | Certificate of Analysis | Jun 17, 2022 | F109384 |
C2225059 | Certificate of Analysis | May 08, 2022 | F109384 |
Solubility | Soluble in chloroform, DMSO, ethanol, and water; solubility :acetone: 25 mg/mL |
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Sensitivity | Air Sensitive |
Melt Point(°C) | 200-203°C |
WGK Germany | 3 |
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Reaxy-Rn | 62575 |
1. Li Shubin, Zhao Yingming, Wu Shuqi, Zhang Xiangxiang, Yang Boyu, Tian Liangfei, Han Xiaojun. (2023) Regulation of species metabolism in synthetic community systems by environmental pH oscillations. Nature Communications, 14 (1): (1-10). [PMID:37980410] |
2. Xuan Li, Xinxin Luo, Ye He, Kun Xu, Yao Ding, Pengfei Gao, Bailong Tao, Meng Li, Meijun Tan, Shaopeng Liu, Peng Liu, Kaiyong Cai. (2023) Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype. ACS Nano, (12): [PMID:37947356] |
3. Yutan Chu, Qiongli Bao, Yan Li, Hongyu Sun, Zewei Liu, Jiahao Shi, Yizong Huang. (2023) Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L.. Antioxidants, 12 (11): (1917). [PMID:38001770] |
4. Yihang Ruan, Huilan Zhuang, Xuemei Zeng, Lili Lin, Xuechun Wang, Panpan Xue, Shan Xu, Qi Chen, Shuangqian Yan, Wei Huang. (2023) Engineered Microbial Nanohybrids for Tumor-Mediated NIR II Photothermal Enhanced Ferroptosis/Cuproptosis and Immunotherapy. Advanced Healthcare Materials, (29): (2302537). [PMID:37742322] |
5. Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan. (2024) Highly bright stable organic radicals encapsulated by amphiphilic polypeptide for efficient near-infrared phototheranostics. TALANTA, 266 (124948). [PMID:37459788] |
6. Wang ShengLiang, Xu ZhiJun, Chen HaiXu, Wang XiaoLiang, Lin Song, Wang Lei, Liu XiaoMan, Huang Xin. (2023) Proliferation-mediated asymmetric nanoencapsulation of single-cell and motility differentiation. Science China-Technological Sciences, 66 (7): (2134-2146). [PMID:] |
7. Qiao Zhang, Jinlin Chen, Yuan Feng, Jingjing Lin, Jiehua Li, Yanchao Wang, Hong Tan. (2023) Electroactive scaffolds of biodegradable polyurethane/polydopamine-functionalized graphene oxide regulating inflammatory response and revitalizing axonal growth cone for peripheral nerve regeneration. Journal of Materials Chemistry B, [PMID:37326438] |
8. Fei Zhou, Yang Yang, Yuying Liu, Haotian Deng, Jianhua Rong, Jianhao Zhao. (2023) A calcium hydroxide/oleic acid/phospholipid nanoparticle induced cancer cell apoptosis by the combination of intracellular calcium overload and lactic acidosis elimination. Journal of Materials Chemistry B, 11 (25): (5856-5869). [PMID:37291978] |
9. Zhikun Yang, Mingrui Li, Yanxiao Li, Xin Wang, Zhihua Li, Jiyong Shi, Xiaowei Huang, Xiaodong Zhai, Xiaobo Zou, Yunyun Gong, Melvin Holmes, Megan Povey, Jianbo Xiao. (2023) Entrapment of probiotic (Bifidobacterium longum) in bilayer emulsion film with enhanced barrier property for improving viability. FOOD CHEMISTRY, 423 (136300). [PMID:37196410] |
10. Zhang Quanchao, Zhang Mengna, Wang Ming, Song Zhiqiang, Luo Honglin, Yang Zhiwei, Wan Yizao. (2023) An Integrated Micro-nano-fibrous Bilayered Small-Diameter Vascular Graft Simultaneously Supporting Endothelial and Smooth Muscle Cells. FIBERS AND POLYMERS, 24 (4): (1211-1223). [PMID:] |
11. Shengliang Wang, Haixu Chen, Zhijun Xu, Xiaoliang Wang, Zhengyu Tao, Lei Wang, Xiaoman Liu, Xin Huang. (2023) Engineering Native Cells by TiO2 Nanoparticles and Polypyrrole for Light-Responsive Manipulation of Collective Behaviors of Unicellular Organisms. ACS Applied Nano Materials, 6 (6): (4626–4635). [PMID:] |
12. Zhang Quanchao, Zhong Bingbing, Zhang Yichuan, Yang Zhiwei, Zhu Xiangbo, Wang Jie, Luo Honglin, Wan Yizao. (2023) Incorporation of chondroitin sulfate into macroporous bacterial cellulose scaffold for improved bioactivity. JOURNAL OF MATERIALS RESEARCH, 38 (8): (2213-2224). [PMID:] |
13. Yuhang Du, Shuna Mi, Huihui Wang, Fangwei Yang, Hang Yu, Yunfei Xie, Yahui Guo, Yuliang Cheng, Weirong Yao. (2023) Inactivation mechanism of Alternaria alternata by dielectric barrier discharge plasma and its quality control on fresh wolfberries. FOOD CONTROL, 148 (109620). [PMID:] |
14. Ziqing Zhong, Xiping Wu, Mindong Bai, Xiaodian Huang, Qilin Zheng, Chunxiang Ai. (2022) Treatments of orange-spotted grouper (Epinephelus coioides) against Cryptocaryon irritans with •OH, ClO2 or HCHO: Survival, physiological and histological response. JOURNAL OF FISH DISEASES, 46 (3): (215-227). [PMID:36519440] |
15. Denghang Xie, Huiwen Wang, Cheng Yin, Mengxia Peng, Haiyong Ao, Jian Hu, Yizao Wan, Quanchao Zhang. (2022) Smart-Temporary-Film-Based Local-Delivery System with Controllable Drug-Release Behavior. Gels, 8 (12): (773). [PMID:36547297] |
16. Run-Ze Hu, Jia-Xin Yang, Zheng-Feng Zhang, Jia-Xing Huang, Yu-Shi Shen, Xiao-Hui Yao, Dong-Yang Zhang, Tao Chen, Yu-Jie Fu. (2023) An all-cellulose sponge with a nanofiller-assisted hierarchical cellular structure for fruit maintaining freshness. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 225 (1361). [PMID:36435456] |
17. Yihan Fu, Fei Ye, Xuwu Zhang, Yuchu He, Xiaoyu Li, Yongfu Tang, Jing Wang, Dawei Gao. (2022) Decrease in Tumor Interstitial Pressure for Enhanced Drug Intratumoral Delivery and Synergistic Tumor Therapy. ACS Nano, 16 (11): (18376–18389). [PMID:36355037] |
18. Yu Wang, Zejun Pei, Zaixiang Lou, Hongxin Wang. (2022) Evaluation of Anti-Biofilm Capability of Cordycepin Against Candida albicans. Infection and Drug Resistance, [PMID:33574683] |
19. Yanjuan Huang, Zilin Guan, Lingling Ren, Yong Luo, Meixu Chen, Yue Sun, Yuanfeng He, Zishan Zeng, Xiuling Dai, Jingwen Jiang, Zeqian Huang, Chunshun Zhao. (2022) Bortezomib prodrug catalytic nanoreactor for chemo/chemodynamic therapy and macrophage re-education. JOURNAL OF CONTROLLED RELEASE, 350 (332). [PMID:36028045] |
20. Zining Hao, Yuchu He, Jing Wang, Xuwu Zhang, Fei Ye, Ze Guan, Xiaokang Liu, Zhenhe Ma, Yi Yuan, Hongming Lou, Dawei Gao. (2022) Photocatalysis/enzymolysis-based biomimetic Schottky junction reduces tumor interstitial solid and fluid phases for deep-penetrating tumor therapy. CHEMICAL ENGINEERING JOURNAL, 446 (137196). [PMID:] |
21. Li Hanrui, Cai Xiaoxia, Yi Tong, Zeng Yun, Ma Jingwen, Li Lei, Pang Liaojun, Li Na, Hu Hao, Zhan Yonghua. (2022) Tumor microenvironment responsive Mn3O4 nanoplatform for in vivo real-time monitoring of drug resistance and photothermal/chemodynamic synergistic therapy of gastric cancer. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-22). [PMID:35606848] |
22. Yan Li, Shengnan Zhang, Qiongli Bao, Yutan Chu, Hongyu Sun, Yizong Huang. (2022) Jasmonic acid alleviates cadmium toxicity through regulating the antioxidant response and enhancing the chelation of cadmium in rice (Oryza sativa L.). ENVIRONMENTAL POLLUTION, 304 (119178). [PMID:35367286] |
23. Qiuxing Liu, Xin Ding, Xiaoyu Xu, Hualu Lai, Zishan Zeng, Ting Shan, Tao Zhang, Meixu Chen, Yanjuan Huang, Zeqian Huang, Xiuling Dai, Meng Xia, Shengmiao Cui. (2022) Tumor-targeted hyaluronic acid-based oxidative stress nanoamplifier with ROS generation and GSH depletion for antitumor therapy. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 207 (771). [PMID:35351548] |
24. Huiping Dang, Youliang Tian, Quan Cheng, Changchang Teng, Kai Xie, Lifeng Yan. (2022) Galactose conjugated boron dipyrromethene and hydrogen bonding promoted J-aggregates for efficiently targeted NIR-II fluorescence assistant photothermal therapy. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 612 (287). [PMID:34995865] |
25. Meixu Chen, Zeqian Huang, Meng Xia, Yaqing Ding, Ting Shan, Zilin Guan, Xiuling Dai, Xiaoyu Xu, Yanjuan Huang, Min Huang, Chunshun Zhao. (2022) Glutathione-responsive copper-disulfiram nanoparticles for enhanced tumor chemotherapy. JOURNAL OF CONTROLLED RELEASE, 341 (351). [PMID:34856225] |
26. Liang-Bin Xiong,Hao-Hao Liu,Ming Zhao,Yong-Jun Liu,Lu Song,Zhi-Yong Xie,Yi-Xin Xu,Feng-Qing Wang,Dong-Zhi Wei. (2020-04-02) Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum.. Microbial cell factories, 19 ((1)): (80-80). [PMID:32228591] |
27. Zeng Q, Nie X, Li L, Liu HF, Peng YY, Zhou WT, Hu XJ, Xu XY, Chen XL.. (2022) Salidroside Promotes Sensitization to Doxorubicin in Human Cancer Cells by Affecting the PI3K/Akt/HIF Signal Pathway and Inhibiting the Expression of Tumor-Resistance-Related Proteins.. J Nat Prod, 85 (1.0): (196-204). [PMID:34978808] |
1. Li Shubin, Zhao Yingming, Wu Shuqi, Zhang Xiangxiang, Yang Boyu, Tian Liangfei, Han Xiaojun. (2023) Regulation of species metabolism in synthetic community systems by environmental pH oscillations. Nature Communications, 14 (1): (1-10). [PMID:37980410] |
2. Xuan Li, Xinxin Luo, Ye He, Kun Xu, Yao Ding, Pengfei Gao, Bailong Tao, Meng Li, Meijun Tan, Shaopeng Liu, Peng Liu, Kaiyong Cai. (2023) Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype. ACS Nano, (12): [PMID:37947356] |
3. Yutan Chu, Qiongli Bao, Yan Li, Hongyu Sun, Zewei Liu, Jiahao Shi, Yizong Huang. (2023) Melatonin Alleviates Antimony Toxicity by Regulating the Antioxidant Response and Reducing Antimony Accumulation in Oryza sativa L.. Antioxidants, 12 (11): (1917). [PMID:38001770] |
4. Yihang Ruan, Huilan Zhuang, Xuemei Zeng, Lili Lin, Xuechun Wang, Panpan Xue, Shan Xu, Qi Chen, Shuangqian Yan, Wei Huang. (2023) Engineered Microbial Nanohybrids for Tumor-Mediated NIR II Photothermal Enhanced Ferroptosis/Cuproptosis and Immunotherapy. Advanced Healthcare Materials, (29): (2302537). [PMID:37742322] |
5. Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan. (2024) Highly bright stable organic radicals encapsulated by amphiphilic polypeptide for efficient near-infrared phototheranostics. TALANTA, 266 (124948). [PMID:37459788] |
6. Wang ShengLiang, Xu ZhiJun, Chen HaiXu, Wang XiaoLiang, Lin Song, Wang Lei, Liu XiaoMan, Huang Xin. (2023) Proliferation-mediated asymmetric nanoencapsulation of single-cell and motility differentiation. Science China-Technological Sciences, 66 (7): (2134-2146). [PMID:] |
7. Qiao Zhang, Jinlin Chen, Yuan Feng, Jingjing Lin, Jiehua Li, Yanchao Wang, Hong Tan. (2023) Electroactive scaffolds of biodegradable polyurethane/polydopamine-functionalized graphene oxide regulating inflammatory response and revitalizing axonal growth cone for peripheral nerve regeneration. Journal of Materials Chemistry B, [PMID:37326438] |
8. Fei Zhou, Yang Yang, Yuying Liu, Haotian Deng, Jianhua Rong, Jianhao Zhao. (2023) A calcium hydroxide/oleic acid/phospholipid nanoparticle induced cancer cell apoptosis by the combination of intracellular calcium overload and lactic acidosis elimination. Journal of Materials Chemistry B, 11 (25): (5856-5869). [PMID:37291978] |
9. Zhikun Yang, Mingrui Li, Yanxiao Li, Xin Wang, Zhihua Li, Jiyong Shi, Xiaowei Huang, Xiaodong Zhai, Xiaobo Zou, Yunyun Gong, Melvin Holmes, Megan Povey, Jianbo Xiao. (2023) Entrapment of probiotic (Bifidobacterium longum) in bilayer emulsion film with enhanced barrier property for improving viability. FOOD CHEMISTRY, 423 (136300). [PMID:37196410] |
10. Zhang Quanchao, Zhang Mengna, Wang Ming, Song Zhiqiang, Luo Honglin, Yang Zhiwei, Wan Yizao. (2023) An Integrated Micro-nano-fibrous Bilayered Small-Diameter Vascular Graft Simultaneously Supporting Endothelial and Smooth Muscle Cells. FIBERS AND POLYMERS, 24 (4): (1211-1223). [PMID:] |
11. Shengliang Wang, Haixu Chen, Zhijun Xu, Xiaoliang Wang, Zhengyu Tao, Lei Wang, Xiaoman Liu, Xin Huang. (2023) Engineering Native Cells by TiO2 Nanoparticles and Polypyrrole for Light-Responsive Manipulation of Collective Behaviors of Unicellular Organisms. ACS Applied Nano Materials, 6 (6): (4626–4635). [PMID:] |
12. Zhang Quanchao, Zhong Bingbing, Zhang Yichuan, Yang Zhiwei, Zhu Xiangbo, Wang Jie, Luo Honglin, Wan Yizao. (2023) Incorporation of chondroitin sulfate into macroporous bacterial cellulose scaffold for improved bioactivity. JOURNAL OF MATERIALS RESEARCH, 38 (8): (2213-2224). [PMID:] |
13. Yuhang Du, Shuna Mi, Huihui Wang, Fangwei Yang, Hang Yu, Yunfei Xie, Yahui Guo, Yuliang Cheng, Weirong Yao. (2023) Inactivation mechanism of Alternaria alternata by dielectric barrier discharge plasma and its quality control on fresh wolfberries. FOOD CONTROL, 148 (109620). [PMID:] |
14. Ziqing Zhong, Xiping Wu, Mindong Bai, Xiaodian Huang, Qilin Zheng, Chunxiang Ai. (2022) Treatments of orange-spotted grouper (Epinephelus coioides) against Cryptocaryon irritans with •OH, ClO2 or HCHO: Survival, physiological and histological response. JOURNAL OF FISH DISEASES, 46 (3): (215-227). [PMID:36519440] |
15. Denghang Xie, Huiwen Wang, Cheng Yin, Mengxia Peng, Haiyong Ao, Jian Hu, Yizao Wan, Quanchao Zhang. (2022) Smart-Temporary-Film-Based Local-Delivery System with Controllable Drug-Release Behavior. Gels, 8 (12): (773). [PMID:36547297] |
16. Run-Ze Hu, Jia-Xin Yang, Zheng-Feng Zhang, Jia-Xing Huang, Yu-Shi Shen, Xiao-Hui Yao, Dong-Yang Zhang, Tao Chen, Yu-Jie Fu. (2023) An all-cellulose sponge with a nanofiller-assisted hierarchical cellular structure for fruit maintaining freshness. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 225 (1361). [PMID:36435456] |
17. Yihan Fu, Fei Ye, Xuwu Zhang, Yuchu He, Xiaoyu Li, Yongfu Tang, Jing Wang, Dawei Gao. (2022) Decrease in Tumor Interstitial Pressure for Enhanced Drug Intratumoral Delivery and Synergistic Tumor Therapy. ACS Nano, 16 (11): (18376–18389). [PMID:36355037] |
18. Yu Wang, Zejun Pei, Zaixiang Lou, Hongxin Wang. (2022) Evaluation of Anti-Biofilm Capability of Cordycepin Against Candida albicans. Infection and Drug Resistance, [PMID:33574683] |
19. Yanjuan Huang, Zilin Guan, Lingling Ren, Yong Luo, Meixu Chen, Yue Sun, Yuanfeng He, Zishan Zeng, Xiuling Dai, Jingwen Jiang, Zeqian Huang, Chunshun Zhao. (2022) Bortezomib prodrug catalytic nanoreactor for chemo/chemodynamic therapy and macrophage re-education. JOURNAL OF CONTROLLED RELEASE, 350 (332). [PMID:36028045] |
20. Zining Hao, Yuchu He, Jing Wang, Xuwu Zhang, Fei Ye, Ze Guan, Xiaokang Liu, Zhenhe Ma, Yi Yuan, Hongming Lou, Dawei Gao. (2022) Photocatalysis/enzymolysis-based biomimetic Schottky junction reduces tumor interstitial solid and fluid phases for deep-penetrating tumor therapy. CHEMICAL ENGINEERING JOURNAL, 446 (137196). [PMID:] |
21. Li Hanrui, Cai Xiaoxia, Yi Tong, Zeng Yun, Ma Jingwen, Li Lei, Pang Liaojun, Li Na, Hu Hao, Zhan Yonghua. (2022) Tumor microenvironment responsive Mn3O4 nanoplatform for in vivo real-time monitoring of drug resistance and photothermal/chemodynamic synergistic therapy of gastric cancer. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-22). [PMID:35606848] |
22. Yan Li, Shengnan Zhang, Qiongli Bao, Yutan Chu, Hongyu Sun, Yizong Huang. (2022) Jasmonic acid alleviates cadmium toxicity through regulating the antioxidant response and enhancing the chelation of cadmium in rice (Oryza sativa L.). ENVIRONMENTAL POLLUTION, 304 (119178). [PMID:35367286] |
23. Qiuxing Liu, Xin Ding, Xiaoyu Xu, Hualu Lai, Zishan Zeng, Ting Shan, Tao Zhang, Meixu Chen, Yanjuan Huang, Zeqian Huang, Xiuling Dai, Meng Xia, Shengmiao Cui. (2022) Tumor-targeted hyaluronic acid-based oxidative stress nanoamplifier with ROS generation and GSH depletion for antitumor therapy. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 207 (771). [PMID:35351548] |
24. Huiping Dang, Youliang Tian, Quan Cheng, Changchang Teng, Kai Xie, Lifeng Yan. (2022) Galactose conjugated boron dipyrromethene and hydrogen bonding promoted J-aggregates for efficiently targeted NIR-II fluorescence assistant photothermal therapy. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 612 (287). [PMID:34995865] |
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