| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab104100-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab104100-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab104100-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab104100-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human Fragilis Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Fragilis Antibody |
|---|---|
| Synonyms | 1-8D | 1-8U | DSPA2b | DSPA2c | IFITM2 | IFITM3 | IP15 | IFITM2/IFITM3 | 1 8U antibody | Fragilis antibody | IFITM3 antibody | IFM3_HUMAN antibody | Interferon induced transmembrane protein 3 (1 8U) antibody | Interferon Induced Transmembrane Protein 3 an |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. |
| Host species | Rabbit |
| Specificity | IFITM3 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IFITM3 |
| Positive Control | WB: Hep G2, RAW 264.7 and NIH/3T3 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human Fragilis Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Fragilis Antibody (Ab104100) - Western Blot
All lanes: Fragilis Antibody (Ab104100) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 100.0 s
Fragilis Antibody (Ab104100) - IF/ICC
Immunofluorescent analysis of IFITM3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Fragilis Antibody (Ab104100) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Fragilis Antibody (Ab104100) - IHC
Immunohistochemical analysis of IFITM3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Fragilis Antibody (Ab104100) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
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