pAb; Rabbit anti Human FXR2 Antibody; WB, IHC, IF/ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab104283-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab104283-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab104283-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab104283-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human FXR2 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | FXR2 Antibody |
|---|---|
| Synonyms | FXR2 | FXR2P | FMR1L2 | FMR1L2 antibody | Fragile X mental retardation 1 like 2 antibody | Fragile X mental retardation autosomal homolog 2 antibody | Fragile X mental retardation gene autosomal homolog 2 antibody | Fragile X mental retardation syndrome r |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | FXR2 |
| Immunogen | A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FXR2. |
| Positive Control | WB: HEK293, HeLa, A549, MCF7 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human FXR2 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
FXR2 Antibody (Ab104283) - Western Blot
All lanes: FXR2 Antibody (Ab104283) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa
Exposure time: 52.5 s
FXR2 Antibody (Ab104283) - Western Blot
All lanes: FXR2 Antibody (Ab104283) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa
Exposure time: 52.5 s
FXR2 Antibody (Ab104283) - IHC
Immunohistochemical analysis of FXR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FXR2 Antibody (Ab104283) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
FXR2 Antibody (Ab104283) - IF/ICC
Immunofluorescent analysis of FXR2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FXR2 Antibody (Ab104283) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| IF/ICC |
1/100-1/500
|
| WB |
1/500-1/1000
|
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