Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Soluble in water (>500 g/l) at 20 °C, methanol, ethanol, and DMSO. Insoluble in benzene, ether, chloroform, and fixed and volatile oils. Absorbs water from the air. Also absorbs hydrogen sulfide, hydrogen cyanide and sulfur dioxide.
H2409059
