Galectin 1 Antibody - Primary antibody, specific to Galectin 1, Rabbit IgG

Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human Galectin 1 Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab104554
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab104554-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$56.90
Ab104554-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$821.90
Ab104554-50μl
50μl
In stock
$169.90
Ab104554-100μl
100μl
In stock
$259.90
Ab104554-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

pAb; Rabbit anti Human Galectin 1 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameGalectin 1 Antibody - Primary antibody, specific to Galectin 1, Rabbit IgG
Synonyms14 kDa laminin-binding protein antibody; 14 kDa lectin antibody; Beta galactoside binding lectin antibody; Beta galactoside binding lectin L 14 I antibody; beta galactoside binding protein antibody; Beta-galactoside-binding lectin L-14-I antibody; DKFZp68
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host speciesRabbit
SpecificityGalectin 1
ImmunogenRecombinant Galectin 1 expressed in E.coli (AA 1-135)
Positive ControlWB: K562, A549, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Human rectum and renal cancer tissues, Rat testis, colon and lung tissues, Porcine colon, uterus, small intestine, cardiac muscle and stomach tissues.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Rabbit anti Human Galectin 1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
Protein Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified and Protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Galectin 1 Antibody (Ab104554) - Western Blot
All lanes: Galectin 1 Antibody (Ab104554) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 2.3 s

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Samples: Human Rectum Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Samples: Human Renal cancer Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Rat Testis Tissue
Primary Ab: 10 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Rat Colon Tissue
Primary Ab: 10 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Rat Lung Tissue
Primary Ab: 10 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Colon Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Uterus Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Small intestine Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Stomach Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Associated Targets(Human)

LGALS1 Tchem Galectin-1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
LGALS1 Tchem Galectin-1 (387 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101527Certificate of AnalysisNov 02, 2023 Ab104554
ZJ23F1101528Certificate of AnalysisNov 02, 2023 Ab104554

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
2. Perillo NL, Pace KE, Seilhamer JJ, Baum LG.  ()  Apoptosis of T cells mediated by galectin-1..  Nature,      [PMID:7501023]
3. Oberg CT, Blanchard H, Leffler H, Nilsson UJ..  (2008)  Protein subtype-targeting through ligand epimerization: talose-selectivity of galectin-4 and galectin-8..  Bioorg Med Chem Lett,  18  (13): (3691-3694).  [PMID:18539029]
4. Delaine T, Cumpstey I, Ingrassia L, Le Mercier M, Okechukwu P, Leffler H, Kiss R, Nilsson UJ..  (2008)  Galectin-inhibitory thiodigalactoside ester derivatives have antimigratory effects in cultured lung and prostate cancer cells..  J Med Chem,  51  (24): (8109-8114).  [PMID:19053747]
5. Dings RP, Miller MC, Nesmelova I, Astorgues-Xerri L, Kumar N, Serova M, Chen X, Raymond E, Hoye TR, Mayo KH..  (2012)  Antitumor agent calixarene 0118 targets human galectin-1 as an allosteric inhibitor of carbohydrate binding..  J Med Chem,  55  (11): (5121-5129).  [PMID:22575017]
6. Rajput VK, MacKinnon A, Mandal S, Collins P, Blanchard H, Leffler H, Sethi T, Schambye H, Mukhopadhyay B, Nilsson UJ..  (2016)  A Selective Galactose-Coumarin-Derived Galectin-3 Inhibitor Demonstrates Involvement of Galectin-3-glycan Interactions in a Pulmonary Fibrosis Model..  J Med Chem,  59  (17): (8141-8147).  [PMID:27500311]
7. Gabr M,Rehman AU,Chen HF.  (2020)  Quinoline-Pyrazole Scaffold as a Novel Ligand of Galectin-3 and Suppressor of TREM2 Signaling..  ACS Med Chem Lett,  11  (9): (1759-1765).  [PMID:32944144]
8. Gitt, M A MA and Barondes, S H SH..  (1991)  Genomic sequence and organization of two members of a human lectin gene family..  Biochemistry,    (8):   [PMID:1988031]
9. Sharma, A A, Chemelli, R R and Allen, H J HJ..  (1990)  Human splenic galaptin: physicochemical characterization..  Biochemistry,    (5):   [PMID:2383549]
10. Abbott, W M WM and Feizi, T T..  (1989)  Evidence that the 14 kDa soluble beta-galactoside-binding lectin in man is encoded by a single gene..  The Biochemical journal,    (1):   [PMID:2719646]
11. Hirabayashi, J J, Ayaki, H H, Soma, G G and Kasai, K K..  (1989)  Cloning and nucleotide sequence of a full-length cDNA for human 14 kDa beta-galactoside-binding lectin..  Biochimica et biophysica acta,    (1):   [PMID:2719964]
12. Couraud, P O PO and 5 more authors..  (1989)  Molecular cloning, characterization, and expression of a human 14-kDa lectin..  The Journal of biological chemistry,    (15):   [PMID:2910856]
13. Hirabayashi, J J and Kasai, K K..  (1988)  Complete amino acid sequence of a beta-galactoside-binding lectin from human placenta..  Journal of biochemistry,      [PMID:3065332]
14. Hirabayashi, J J, Kawasaki, H H, Suzuki, K K and Kasai, K K..  (1987)  Further characterization and structural studies on human placenta lectin..  Journal of biochemistry,      [PMID:3611046]
15. Dunham, I I and 24 more authors..  (1999)  The DNA sequence of human chromosome 22..  Nature,    (2):   [PMID:10591208]
16. Fouillit, M M and 8 more authors..  (2000)  Regulation of CD45-induced signaling by galectin-1 in Burkitt lymphoma B cells..  Glycobiology,      [PMID:10764829]
17. Tinari, N N and 5 more authors..  (2001)  Glycoprotein 90K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation..  International journal of cancer,    (15):   [PMID:11146440]
18. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,      [PMID:12665801]
19. Matsuda, Akio A and 11 more authors..  (2003)  Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways..  Oncogene,    (22):   [PMID:12761501]
20. He, Jiale J and Baum, Linda G LG..  (2004)  Presentation of galectin-1 by extracellular matrix triggers T cell death..  The Journal of biological chemistry,    (6):   [PMID:14617626]
21. Collins, John E JE and 12 more authors..  (2004)  A genome annotation-driven approach to cloning the human ORFeome..  Genome biology,      [PMID:15461802]
22. Nishi, Nozomu N and 8 more authors..  (2008)  Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1..  Glycobiology,      [PMID:18796645]
23. Than, Nandor Gabor NG and 17 more authors..  (2008)  Emergence of hormonal and redox regulation of galectin-1 in placental mammals: implication in maternal-fetal immune tolerance..  Proceedings of the National Academy of Sciences of the United States of America,    (14):   [PMID:18824694]
24. Di Lella, Santiago S and 5 more authors..  (2009)  Critical role of the solvent environment in galectin-1 binding to the disaccharide lactose..  Biochemistry,    (3):   [PMID:19128029]
25. Than, Nandor Gabor NG and 24 more authors..  (2009)  A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death..  Proceedings of the National Academy of Sciences of the United States of America,    (16):   [PMID:19497882]
26. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,      [PMID:22223895]
27. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:22814378]
28. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,    (7):   [PMID:22905912]
29. Watson, Allison P AP, Evans, Rick L RL and Egland, Kristi A KA..  (2013)  Multiple functions of sushi domain containing 2 (SUSD2) in breast tumorigenesis..  Molecular cancer research : MCR,      [PMID:23131994]
30. Escoda-Ferran, Cristina C and 8 more authors..  (2014)  Modulation of CD6 function through interaction with Galectin-1 and -3..  FEBS letters,    (25):   [PMID:24945728]
31. Tagliabracci, Vincent S VS and 14 more authors..  (2015)  A Single Kinase Generates the Majority of the Secreted Phosphoproteome..  Cell,    (18):   [PMID:26091039]
32. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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