pAb; Rabbit anti Human Galectin 3 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab104584-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab104584-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $90.90 | |
| Ab104584-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab104584-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $279.90 | |
| Ab104584-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
pAb; Rabbit anti Human Galectin 3 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Galectin 3 Antibody - Primary antibody, specific to Galectin 3, Rabbit IgG |
|---|---|
| Synonyms | 35 kDa lectin antibody; Carbohydrate binding protein 35 antibody; Carbohydrate-binding protein 35 antibody; CBP 35 antibody; CBP35 antibody; Gal-3 antibody; GAL3 antibody; Galactose-specific lectin 3 antibody; Galactoside-binding protein antibody; GALBP a |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | Galectin 3 |
| Immunogen | Recombinant Galectin 3 expressed in E.coli (AA 1-250) |
| Positive Control | WB: C6, NIH/3T3, Hep G2, A549, HeLa, MCF7 and A431 cell lysates. IHC: Human colorectal cancer, lung cancer, ovarian cancer tissues; Porcine small intestine, kidney and spleen tissues. IHC: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Rabbit anti Human Galectin 3 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified and Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Galectin 3 Antibody (Ab104584) - Western Blot
All lanes: Galectin 3 Antibody (Ab104584) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 29 kDa
Exposure time: 0.1 s
Galectin 3 Antibody (Ab104584) - IF
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 μg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 1 μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 μg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Samples: Human Ovarian cancer Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Sample: Porcine Small intestine Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Sample: Porcine Kidney Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC
DAB staining on IHC
Sample: Porcine Spleen Tissue
Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| ICC/IF |
5-20 µg/mL
|
| WB | 0.1-2 µg/mL |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001225 | Certificate of Analysis | Oct 10, 2023 | Ab104584 |
| ZJ23F1001224 | Certificate of Analysis | Oct 10, 2023 | Ab104584 |
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| 13. Seetharaman, J J and 5 more authors.. (1998) X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.. The Journal of biological chemistry, (22): [PMID:9582341] |
| 14. Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6): [PMID:12508121] |
| 15. Fukushi, Jun-ichi J, Makagiansar, Irwan T IT and Stallcup, William B WB.. (2004) NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and alpha3beta1 integrin.. Molecular biology of the cell, [PMID:15181153] |
| 16. Seidel, Ronald D RD, Zhuang, Tiandi T and Prestegard, James H JH.. (2007) Bound-state residual dipolar couplings for rapidly exchanging ligands of His-tagged proteins.. Journal of the American Chemical Society, (18): [PMID:17385862] |
| 17. Than, Nandor Gabor NG and 24 more authors.. (2009) A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:19497882] |
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| 20. Escoda-Ferran, Cristina C and 8 more authors.. (2014) Modulation of CD6 function through interaction with Galectin-1 and -3.. FEBS letters, (25): [PMID:24945728] |
| 21. Chauhan, Santosh S and 11 more authors.. (2016) TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis.. Developmental cell, (10): [PMID:27693506] |
