| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab155825-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | In stock | $49.90 | |
| Ab155825-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $149.90 | |
| Ab155825-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $219.90 | |
| Ab155825-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,699.90 |
mAb (B08/4A11); Mouse anti Human GAPDH Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| Product Name | GAPDH Mouse mAb - Primary antibody, specific to GAPDH, IgG1 |
|---|---|
| Synonyms | 38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | GAPDH |
| Immunogen | Recombinant full length human GAPDH |
| Positive Control | WB: HEK293, HeLa, Jurkat, A431, HepG2 and K562 whole cell lysates; Flow: Jurkat cells; ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Application |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
GAPDH Mouse mAb (Ab155825) - Western Blot
All lanes: GAPDH Antibody (Ab155825) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 35.8 kDa
Observed band size: 35.6 kDa
Exposure time: 36.9 s
GAPDH Mouse mAb (Ab155825) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with GAPDH Mouse mAb (Ab155825) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.
GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL and Recombinant COX IV Antibody (Ab097410) (Red) at 1μg/mL in blocking buffer for 2h at room temperature and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 and Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147) for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC |
0.5 μg/mL
|
| WB | 1/1000-1/100000 ; Predicted molecular weight: 37 kDa |
| Flow Cytometry | 0.016 μg/mL- 1 μg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404297 | Certificate of Analysis | Apr 09, 2024 | Ab155825 |
| ZJ23F1001304 | Certificate of Analysis | Oct 16, 2023 | Ab155825 |
| ZJ23F1001305 | Certificate of Analysis | Oct 16, 2023 | Ab155825 |
| 1. Verlinde CL, Callens M, Van Calenbergh S, Van Aerschot A, Herdewijn P, Hannaert V, Michels PA, Opperdoes FR, Hol WG.. (1994) Selective inhibition of trypanosomal glyceraldehyde-3-phosphate dehydrogenase by protein structure-based design: toward new drugs for the treatment of sleeping sickness.. J Med Chem, 37 (21): (3605-3613). [PMID:7932587] [10.1021/jm00047a017] |
| 2. Van Calenbergh S, Verlinde CL, Soenens J, De Bruyn A, Callens M, Blaton NM, Peeters OM, Rozenski J, Hol WG, Herdewijn P.. (1995) Synthesis and structure-activity relationships of analogs of 2'-deoxy-2'-(3-methoxybenzamido)adenosine, a selective inhibitor of trypanosomal glycosomal glyceraldehyde-3-phosphate dehydrogenase.. J Med Chem, 38 (19): (3838-3849). [PMID:7562915] [10.1021/jm00019a014] |
| 3. Aronov AM, Verlinde CL, Hol WG, Gelb MH.. (1998) Selective tight binding inhibitors of trypanosomal glyceraldehyde-3-phosphate dehydrogenase via structure-based drug design.. J Med Chem, 41 (24): (4790-4799). [PMID:9822549] [10.1021/jm9802620] |
| 4. Sidique S, Ardecky R, Su Y, Narisawa S, Brown B, Millán JL, Sergienko E, Cosford ND.. (2009) Design and synthesis of pyrazole derivatives as potent and selective inhibitors of tissue-nonspecific alkaline phosphatase (TNAP).. Bioorg Med Chem Lett, 19 (1): (222-225). [PMID:19038545] [10.1016/j.bmcl.2008.10.107] |
| 5. Ramsey DM, McConnell JR, Alexander LD, Tanaka KW, Vera CM, McAlpine SR.. (2012) An Hsp90 modulator that exhibits a unique mechanistic profile.. Bioorg Med Chem Lett, 22 (9): (3287-3290). [PMID:22480433] [10.1016/j.bmcl.2012.03.012] |
| 6. Basudhar D, Bharadwaj G, Cheng RY, Jain S, Shi S, Heinecke JL, Holland RJ, Ridnour LA, Caceres VM, Spadari-Bratfisch RC, Paolocci N, Velázquez-Martínez CA, Wink DA, Miranda KM.. (2013) Synthesis and chemical and biological comparison of nitroxyl- and nitric oxide-releasing diazeniumdiolate-based aspirin derivatives.. J Med Chem, 56 (20): (7804-7820). [PMID:24102516] [10.1021/jm400196q] |
| 7. Bruno S, Pinto A, Paredi G, Tamborini L, De Micheli C, La Pietra V, Marinelli L, Novellino E, Conti P, Mozzarelli A.. (2014) Discovery of covalent inhibitors of glyceraldehyde-3-phosphate dehydrogenase, a target for the treatment of malaria.. J Med Chem, 57 (17): (7465-7471). [PMID:25137375] [10.1021/jm500747h] |
| 8. Long MJC, Lawson AP, Baggio R, Qian Y, Rozhansky L, Fasci D, El Oualid F, Weerapana E, Hedstrom L.. (2019) Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor.. Bioorg Med Chem Lett, 29 (2): (204-211). [PMID:30528168] [10.1016/j.bmcl.2018.11.055] |
| 9. Byun WS, Bae ES, Park SC, Kim WK, Shin J, Lee SK.. (2021) Antitumor Activity of Asperphenin B by Induction of Apoptosis and Regulation of Glyceraldehyde-3-phosphate Dehydrogenase in Human Colorectal Cancer Cells.. J Nat Prod, 84 (3.0): (683-693). [PMID:33398999] [10.1021/acs.jnatprod.0c01155] |
| 10. Mercer, W D WD, Winn, S I SI and Watson, H C HC.. (1976) Twinning in crystals of human skeletal muscle D-glyceraldehyde-3-phosphate dehydrogenase.. Journal of molecular biology, (14): [PMID:957435] |
| 11. Nowak, K K and 5 more authors.. (1975) The covalent structure of glyceraldehyde-phosphate dehydrogenase from human muscles. Isolation and amino acid sequences of peptides from tryptic digest.. Hoppe-Seyler's Zeitschrift fur physiologische Chemie, [PMID:1193541] |
| 12. Meyer-Siegler, K K and 5 more authors.. (1991) A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1924305] |
| 13. Tso, J Y JY, Sun, X H XH, Kao, T H TH, Reece, K S KS and Wu, R R.. (1985) Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.. Nucleic acids research, (11): [PMID:2987855] |
| 14. Allen, R W RW, Trach, K A KA and Hoch, J A JA.. (1987) Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase.. The Journal of biological chemistry, (15): [PMID:3027061] |
| 15. Ercolani, L L, Florence, B B, Denaro, M M and Alexander, M M.. (1988) Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene.. The Journal of biological chemistry, (25): [PMID:3170585] |
| 16. Tokunaga, K K and 6 more authors.. (1987) Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers.. Cancer research, (1): [PMID:3664468] |
| 17. Hanauer, A A and Mandel, J L JL.. (1984) The glyceraldehyde 3 phosphate dehydrogenase gene family: structure of a human cDNA and of an X chromosome linked pseudogene; amazing complexity of the gene family in mouse.. The EMBO journal, [PMID:6096136] |
| 18. Arcari, P P, Martinelli, R R and Salvatore, F F.. (1984) The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species.. Nucleic acids research, (11): [PMID:6096821] |
| 19. Nowak, K K, Wolny, M M and Banaś, T T.. (1981) The complete amino acid sequence of human muscle glyceraldehyde 3-phosphate dehydrogenase.. FEBS letters, (16): [PMID:7030790] |
| 20. Kovalyov, L I LI and 6 more authors.. (1995) The major protein expression profile and two-dimensional protein database of human heart.. Electrophoresis, [PMID:7498159] |
| 21. Ye, Z Z and Connor, J R JR.. (2000) cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs.. Biochemical and biophysical research communications, (18): [PMID:10944468] |
| 22. and Tisdale, Ellen J EJ.. (2002) Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway.. The Journal of biological chemistry, (1): [PMID:11724794] |
| 23. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 24. Mazzola, Jennifer L JL and Sirover, Michael A MA.. (2003) Subcellular localization of human glyceraldehyde-3-phosphate dehydrogenase is independent of its glycolytic function.. Biochimica et biophysica acta, (20): [PMID:12829261] |
| 25. She, Yi-Min YM and 5 more authors.. (2003) Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry.. Molecular & cellular proteomics : MCP, [PMID:14534351] |
| 26. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 27. Sampath, Prabha P and 10 more authors.. (2004) Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation.. Cell, (15): [PMID:15479637] |
| 28. Wakasugi, Keisuke K, Nakano, Tomomi T and Morishima, Isao I.. (2005) Oxidative stress-responsive intracellular regulation specific for the angiostatic form of human tryptophanyl-tRNA synthetase.. Biochemistry, (11): [PMID:15628863] |
| 29. Ismail, S A SA and Park, H W HW.. (2005) Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase.. Acta crystallographica. Section D, Biological crystallography, [PMID:16239728] |
| 30. Bosch-Comas, A A, Lindsten, K K, Gonzàlez-Duarte, R R, Masucci, M G MG and Marfany, G G.. (2006) The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins.. Cellular and molecular life sciences : CMLS, [PMID:16501887] |
| 31. Jenkins, Jermaine L JL and Tanner, John J JJ.. (2006) High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase.. Acta crystallographica. Section D, Biological crystallography, [PMID:16510976] |
| 32. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075] |
| 33. Wong, Joyce Jing Yi JJ, Pung, Yuh Fen YF, Sze, Newman Siu-Kwan NS and Chin, Keh-Chuang KC.. (2006) HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:16815975] |
| 34. Markova, E V EV and 6 more authors.. () [Lymphocyte metabolism in patients with acute pancreatitis with different genotypes of GSTM1 and GSTT1 genes].. Biomeditsinskaia khimiia, [PMID:16898590] |
| 35. and Ido, Yasuo Y.. (2007) Pyridine nucleotide redox abnormalities in diabetes.. Antioxidants & redox signaling, [PMID:17508915] |
| 36. Jarczowski, Franziska F and 5 more authors.. (2009) FKBP36 is an inherent multifunctional glyceraldehyde-3-phosphate dehydrogenase inhibitor.. The Journal of biological chemistry, (9): [PMID:19001379] |
| 37. Rakitina, T V TV and 5 more authors.. () [Haponin (eIF1AD) interacts with glyceraldehyde 3-phosphate dehydrogenase in the CHO-K1 cell line].. Bioorganicheskaia khimiia, [PMID:20644585] |
| 38. Peng, Chao C and 18 more authors.. (2011) The first identification of lysine malonylation substrates and its regulatory enzyme.. Molecular & cellular proteomics : MCP, [PMID:21908771] |
| 39. Arif, Abul A, Chatterjee, Piyali P, Moodt, Robyn A RA and Fox, Paul L PL.. (2012) Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages.. Molecular and cellular biology, [PMID:23071094] |
| 40. Gao, Xiaofei X and 9 more authors.. (2013) NleB, a bacterial effector with glycosyltransferase activity, targets GAPDH function to inhibit NF-κB activation.. Cell host & microbe, (16): [PMID:23332158] |
| 41. Samson, Andre L AL and 9 more authors.. (2014) Oxidation of an exposed methionine instigates the aggregation of glyceraldehyde-3-phosphate dehydrogenase.. The Journal of biological chemistry, (26): [PMID:25086035] |
| 42. Jia, Jie J and 6 more authors.. (2014) Target-selective protein S-nitrosylation by sequence motif recognition.. Cell, (23): [PMID:25417112] |
| 43. Gao, Xiaofei X and 8 more authors.. (2016) Citrobacter rodentium NleB Protein Inhibits Tumor Necrosis Factor (TNF) Receptor-associated Factor 3 (TRAF3) Ubiquitination to Reduce Host Type I Interferon Production.. The Journal of biological chemistry, (26): [PMID:27387501] |
